Cdyl (NM_001123386) Mouse Tagged ORF Clone

CAT#: MR208673

Cdyl (Myc-DDK-tagged) - Mouse chromodomain protein, Y chromosome-like (Cdyl), transcript variant 2

Product Images

Circular map for MR208673

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cdyl
• Synonyms: AI325931
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208673 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCCGAGGAGCTGTACGAGGTGGAAAGTATCGTTGACAAAAGGAAAAACAAGAAAGGGAAGACAG
  AATATCTGGTGCGGTGGAAAGGCTATGACAGTGAGGATGACACGTGGGAGCCTGAGCAGCACCTGGTGAA
  CTGTGAGGAATACATCCATGACTTCAACCGGCGCCACAACGAGAGGCAAAAGGAAGGTAGCCTGGCTCGT
  GCCAGCAGAGCCTCCCCCAGCAACGCCCGGAAGCAGATTTCCAGGTCCACCCACAGCACTCTCTCCAAGA
  CCAACTCCAAAGCACTTGTGGTAGGCAAAGATCATGAGTCCAAAAGCAGCCAGCTGTTGGCTGCCAGCCA
  GAAGTTCAGGAAAAACCCAGCCCCATCTCTTGCAAACCGCAAGAACATGGACCTCGCCAAGTCAGGGATC
  AAAATTCTCGTGCCTAAGAGCCCCGTTAAGGGCAGGACCTCGGTTGATGGCTTTCAGGGGGAGAGCCCCG
  AGAAGCTGGACCCTGTGGATCAGGGTGCCGAGGACACTGTAGCCCCAGAGGTGACTGCAGAGAAGCCCAC
  TGGGGCTTTGCTGGGCCCTGGTGCGGAGCGAGCCAGGATGGGGAGCAGGCCCCGAATACATCCACTAGTG
  CCTCAGGTTTCTGGCCCCGTGACTGCTGCCATGGCCACAGGCTTAGCTGTTAATGGAAAAGGTACATCTC
  CATTCATGGATGCGCTAGCAGCCAACGGAACAGTCACCATACAGACATCCGTAACAGGAGTGACAGCCGG
  GAAAAGGAAATTTATTGACGACAGAAGAGACCAACCTTTTGACAAGCGGTTGCGTTTCAGTGTGAGGCAG
  ACAGAGAGTGCCTACAGATACAGAGATATTGTCGTCAGGAAGCAAGATGGCTTCACCCACATCTTGTTAT
  CCACAAAATCGTCAGAGAATAACTCACTAAACCCAGAGGTGATGAAAGAAGTGCAGAGCGCCCTGAGCAC
  AGCTGCAGCCGACGACAGCAAGCTGGTTCTGCTCAGCGCCGTGGGCAGCGTCTTCTGCTGTGGTCTGGAC
  TTTATTTATTTTATTCGGCGCCTCACAGATGACCGAAAGAGAGAAAGCACTAAAATGGCAGACGCTATCA
  GAAACTTCGTGAATACTTTCATTCAGTTTAAGAAGCCTATTATTGTAGCTGTTAATGGCCCAGCCATTGG
  ACTAGGAGCATCCATATTGCCTCTTTGTGATGTGGTTTGGGCTAACGAAAAGGCTTGGTTTCAAACACCC
  TATACCACCTTCGGACAGAGTCCAGATGGCTGCTCTACCGTTATGTTTCCCAAGATTATGGGAGGAGCAT
  CTGCGAATGAAATGCTGTTCAGTGGGCGGAAGTTGACGGCACAGGAGGCCTGTGGCAAGGGTCTGGTCTC
  CCAGGTGTTTTGGCCAGGAACCTTCACACAGGAAGTCATGGTTCGAATCAAGGAGCTGGCTTCATGTAAC
  CCAGTTGTCCTGGAGGAATCCAAAGCCCTGGTGCGCTGCAATATGAAGATGGAGCTAGAGCAGGCCAATG
  AGAGAGAATGTGAAGTGCTGAAGAAGATCTGGGGCTCCGCCCAGGGCATGGACTCCATGTTAAAGTACTT
  ACAGAGGAAAATCGATGAGTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208673 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASEELYEVESIVDKRKNKKGKTEYLVRWKGYDSEDDTWEPEQHLVNCEEYIHDFNRRHNERQKEGSLAR
  ASRASPSNARKQISRSTHSTLSKTNSKALVVGKDHESKSSQLLAASQKFRKNPAPSLANRKNMDLAKSGI
  KILVPKSPVKGRTSVDGFQGESPEKLDPVDQGAEDTVAPEVTAEKPTGALLGPGAERARMGSRPRIHPLV
  PQVSGPVTAAMATGLAVNGKGTSPFMDALAANGTVTIQTSVTGVTAGKRKFIDDRRDQPFDKRLRFSVRQ
  TESAYRYRDIVVRKQDGFTHILLSTKSSENNSLNPEVMKEVQSALSTAAADDSKLVLLSAVGSVFCCGLD
  FIYFIRRLTDDRKRESTKMADAIRNFVNTFIQFKKPIIVAVNGPAIGLGASILPLCDVVWANEKAWFQTP
  YTTFGQSPDGCSTVMFPKIMGGASANEMLFSGRKLTAQEACGKGLVSQVFWPGTFTQEVMVRIKELASCN
  PVVLEESKALVRCNMKMELEQANERECEVLKKIWGSAQGMDSMLKYLQRKIDEF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001123386
• ORF Size: 1635 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001123386.1, NP_001116858.1
• RefSeq Size: 3494 bp
• RefSeq ORF: 1635 bp
• Locus ID: 12593
• UniProt ID: Q9WTK2
• Cytogenetics: 13 14.39 cM
• MW: 60.1 kDa
• Gene Summary: Isoform 2: Chromatin reader protein that recognizes and binds histone H3 trimethylated at 'Lys-9', dimethylated at 'Lys-27' and trimethylated at 'Lys-27' (H3K9me3, H3K27me2 and H3K27me3, respectively) (PubMed:12947414). Part of multimeric repressive chromatin complexes, where it is required for transmission and restoration of repressive histone marks, thereby preserving the epigenetic landscape (PubMed:12947414). Required for chromatin targeting and maximal enzymatic activity of Polycomb repressive complex 2 (PRC2); acts as a positive regulator of PRC2 activity by bridging the pre-existing histone H3K27me3 and newly recruited PRC2 on neighboring nucleosomes (By similarity). Acts as a corepressor for REST by facilitating histone-lysine N-methyltransferase EHMT2 recruitment and H3K9 dimethylation at REST target genes for repression (By similarity). Involved X chromosome inactivation in females: recruited to Xist RNA-coated X chromosome and facilitates propagation of H3K9me2 by anchoring EHMT2 (PubMed:24144980). Required for neuronal migration during brain development by repressing expression of RHOA (PubMed:28076783). In addition to act as a chromatin reader, acts as a hydro-lyase (By similarity). Shows crotonyl-coA hydratase activity by mediating the conversion of crotonyl-CoA ((2E)-butenoyl-CoA) to beta-hydroxybutyryl-CoA (3-hydroxybutanoyl-CoA), thereby acting as a negative regulator of histone crotonylation (By similarity). Histone crotonylation is required during spermatogenesis; down-regulation of histone crotonylation by CDYL regulates the reactivation of sex chromosome-linked genes in round spermatids and histone replacement in elongating spermatids (PubMed:28803779). May have histone acetyltransferase activity; such activity is however unsure in vivo (PubMed:12072557).[UniProtKB/Swiss-Prot Function]