Ahcyl1 (NM_145542) Mouse Tagged ORF Clone

CAT#: MR208502

Ahcyl1 (Myc-DDK-tagged) - Mouse S-adenosylhomocysteine hydrolase-like 1 (Ahcyl1)

Product Images

Circular map for MR208502

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ahcyl1
• Synonyms: 1110034F20Rik; AA409031; AA414901; Ahcy-rs3; DCAL; Irbit
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208502 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGATGCCTGACGCGATGCCGCTGCCCGGTGTCGGGGAGGAGCTGAAACAGGCCAAGGAGATCGAGG
  ACGCCGAGAAGTACTCCTTCATGGCCACGGTCACCAAGGCTCCCAAGAAGCAAATCCAGTTTGCTGATGA
  CATGCAAGAGTTCACCAAATTCCCTACTAAGACTGGCCGGAGATCTTTGTCTCGTTCCATCTCACAATCC
  TCCACAGACAGCTACAGTTCAGCTGCATCCTATACAGATAGCTCTGATGATGAGGTTTCCCCCCGAGAGA
  AGCAGCAAACCAACTCGAAGGGCAGCAGCAATTTCTGTGTGAAGAACATCAAGCAGGCAGAGTTTGGACG
  CCGGGAGATTGAGATTGCAGAGCAAGACATGTCTGCTCTGATTTCACTCAGGAAACGTGCTCAGGGAGAG
  AAGCCTTTGGCTGGTGCTAAAATAGTGGGCTGTACACACATCACGGCCCAGACAGCGGTATTAATTGAGA
  CCCTTTGTGCCCTGGGAGCTCAGTGCCGCTGGTCTGCCTGCAACATCTATTCAACTCAGAATGAAGTAGC
  TGCAGCACTGGCTGAGGCTGGAGTCGCGGTGTTTGCTTGGAAGGGCGAGTCAGAAGATGATTTCTGGTGG
  TGCATTGACCGCTGTGTCAACATGGATGGGTGGCAGGCTAACATGATCCTGGATGATGGGGGAGACTTAA
  CCCACTGGGTTTATAAGAAGTATCCAAACGTGTTTAAGAAGATCCGAGGCATTGTGGAAGAGAGCGTGAC
  TGGTGTTCACAGGCTGTATCAGCTCTCCAAAGCTGGGAAGCTCTGTGTTCCAGCCATGAATGTCAATGAT
  TCTGTTACCAAACAGAAGTTTGATAACCTGTACTGCTGCCGAGAATCCATTTTGGATGGCCTGAAGAGGA
  CCACGGATGTGATGTTTGGTGGGAAACAGGTGGTGGTGTGTGGCTATGGTGAGGTAGGAAAGGGCTGCTG
  TGCTGCTCTCAAGGCCCTTGGAGCAATTGTCTACATCACAGAAATTGACCCCATCTGTGCTCTGCAGGCC
  TGCATGGATGGGTTCAGGGTGGTGAAGCTGAATGAAGTCATCCGGCAGGTGGACGTTGTAATTACTTGCA
  CAGGAAATAAGAATGTAGTGACTCGGGAGCACTTGGACCGAATGAAAAATAGTTGTATTGTGTGCAATAT
  GGGCCATTCCAACACGGAGATCGATGTGACCAGCCTCCGCACTCCAGAACTAACATGGGAGCGTGTACGT
  TCTCAGGTGGACCATGTCATCTGGCCTGATGGCAAACGGGTCGTCCTTCTAGCAGAGGGCCGTTTACTTA
  ATCTGAGCTGCTCCACAGTTCCTACCTTTGTTCTTTCCATCACGGCTACAACACAGGCTTTGGCACTGAT
  AGAGCTTTACAACGCCCCGGAGGGACGCTACAAACAGGATGTGTACTTGCTTCCTAAGAAGATGGATGAA
  TATGTTGCCAGCTTGCACCTACCATCATTTGATGCCCACCTGACAGAACTGACAGATGACCAAGCAAAGT
  ATCTGGGACTCAACAAAAATGGGCCATTCAAACCTAATTATTACAGATAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208502 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSMPDAMPLPGVGEELKQAKEIEDAEKYSFMATVTKAPKKQIQFADDMQEFTKFPTKTGRRSLSRSISQS
  STDSYSSAASYTDSSDDEVSPREKQQTNSKGSSNFCVKNIKQAEFGRREIEIAEQDMSALISLRKRAQGE
  KPLAGAKIVGCTHITAQTAVLIETLCALGAQCRWSACNIYSTQNEVAAALAEAGVAVFAWKGESEDDFWW
  CIDRCVNMDGWQANMILDDGGDLTHWVYKKYPNVFKKIRGIVEESVTGVHRLYQLSKAGKLCVPAMNVND
  SVTKQKFDNLYCCRESILDGLKRTTDVMFGGKQVVVCGYGEVGKGCCAALKALGAIVYITEIDPICALQA
  CMDGFRVVKLNEVIRQVDVVITCTGNKNVVTREHLDRMKNSCIVCNMGHSNTEIDVTSLRTPELTWERVR
  SQVDHVIWPDGKRVVLLAEGRLLNLSCSTVPTFVLSITATTQALALIELYNAPEGRYKQDVYLLPKKMDE
  YVASLHLPSFDAHLTELTDDQAKYLGLNKNGPFKPNYYRY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145542
• ORF Size: 1593 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145542.1
• RefSeq Size: 3866 bp
• RefSeq ORF: 1593 bp
• Locus ID: 229709
• UniProt ID: Q80SW1
• Cytogenetics: 3 46.83 cM
• MW: 59 kDa
• Gene Summary: Multifaceted cellular regulator which coordinates several essential cellular functions including regulation of epithelial HCO3(-) and fluid secretion, mRNA processing and DNA replication. Regulates ITPR1 sensitivity to inositol 1,4,5-trisphosphate competing for the common binding site and acting as endogenous 'pseudoligand' whose inhibitory activity can be modulated by its phosphorylation status. In the pancreatic and salivary ducts, at resting state, attenuates inositol 1,4,5-trisphosphate-induced calcium release by interacting with ITPR1 (By similarity). When extracellular stimuli induce ITPR1 phosphorylation or inositol 1,4,5-trisphosphate production, dissociates of ITPR1 to interact with CFTR and SLC26A6 mediating their synergistic activation by calcium and cAMP that stimulates the epithelial secretion of electrolytes and fluid (PubMed:12525476, PubMed:23542070). Also activates basolateral SLC4A4 isoform 1 to coordinate fluid and HCO3(-) secretion (PubMed:19224921). Inhibits the effect of STK39 on SLC4A4 and CFTR by recruiting PP1 phosphatase which activates SLC4A4, SLC26A6 and CFTR through dephosphorylation (PubMed:19033647, PubMed:21317537). Mediates the induction of SLC9A3 surface expression produced by Angiotensin-2. Depending on the cell type, activates SLC9A3 in response to calcium or reverses SLC9A3R2-dependent calcium inhibition. May modulate the polyadenylation state of specific mRNAs, both by controlling the subcellular location of FIP1L1 and by inhibiting PAPOLA activity, in response to a stimulus that alters its phosphorylation state. Acts as a (dATP)-dependent inhibitor of ribonucleotide reductase large subunit RRM1, controlling the endogenous dNTP pool and ensuring normal cell cycle progression (By similarity). In vitro does not exhibit any S-adenosyl-L-homocysteine hydrolase activity (PubMed:12525476).[UniProtKB/Swiss-Prot Function]