Umps (NM_009471) Mouse Tagged ORF Clone

CAT#: MR207718

Umps (Myc-DDK-tagged) - Mouse uridine monophosphate synthetase (Umps)

Product Images

Circular map for MR207718

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Umps
• Synonyms: 1700095D23Rik; AA408257; AL033308; BB164745
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207718 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGTCGCCAGCCAAGCCCTGGGCCCCTTGGTCACCGAGCTGTATGACGTGCAGGCTTTCAAGTTCG
  GGAGCTTCGTCCTGAAGAGCGGGCTCTCCTCCCCAGTCTACATCGACCTGCGGGGCATCGTGTCACGCCC
  GCGTCTTCTCAGTCAGGTCGCAGACATTTTATTCCAGACTGCAAAAAATGCGGGGATCAGTTTTGACAGT
  GTGTGTGGAGTTCCTTATACAGCGTTACCACTGGCGACAGTTATCTGCTCAGCCAATCACATTCCCATGC
  TCATTAGGAGGAAGGAAACAAAGGATTATGGTACCAAGCGCCTTGTAGAAGGAGAGATTAATCCGGGCCA
  GACCTGTCTGGTCATTGAGGACGTCGTCACCAGCGGAGCCAGTGTTTTGGAGACTGTGGAAGTTCTTCAG
  AAGGAGGGTCTAAAGGTAACTGATGCCATAGTGCTGTTGGACCGGGAGCAGGGAGGCAAGGACAAGCTGC
  AGGCCCAGGGGATCCGCCTCCACGCCGTGTGCACGCTCTCCCAAATGCTGGAGATTCTCCAGCAGCAGGA
  AAAGATCGACGCTGACATGGTCGGGAGAGTGAAGAGGTTCATTCAGGAGAACGTTTTCTCAGCAGCTAAT
  CATAACGGTCTCCCCCCTCCTGAGAAGAAAGCGTGCAAAGAACTCAGCTTTGGTGCTCGAGCTGAGCTGC
  CTGGAACCCACCCGCTTGCCTCAAAGCTGCTCCGGCTCATGCAGAAGAAGGAGACCAATCTGTGTCTGTC
  TGCCGATGTGTCGGAGGCCAGGGAGCTGCTGCAGCTCGCAGACGCCTTGGGACCCAGCATCTGCATGCTC
  AAAACTCATGTCGACATTCTGAATGATTTTACTCTGGATGTGATGGAGGAGTTGACAGCCCTGGCAAAAC
  GCCACGAGTTCTTAATATTTGAAGACAGGAAATTCGCTGATATTGGAAACACAGTTAAAAAGCAGTATGA
  AAGTGGCACCTTTAAAATAGCTTCATGGGCAGATATAGTGAATGCTCACGTCGTGCCAGGCTCAGGAGTT
  GTGAAAGGCTTACAGGAAGTGGGTCTGCCTTTACATCGAGCCTGCCTGCTCATTGCAGAAATGAGCTCTG
  CTGGCTCCTTGGCCACTGGAAACTACACCAAGGCAGCAGTCGGGATGGCTGAGGAGCACTGTGAATTTGT
  CATTGGCTTCATTTCTGGCTCCCGAGTAAGCATGAAACCAGAGTTTCTTCACTTGACCCCAGGAGTTCAG
  TTAGAAACAGGAGGGGATCACCTTGGCCAGCAGTACAATAGTCCCCAAGAAGTGATCGGGAAACGCGGTT
  CTGATGTCATCATTGTAGGCCGGGGAATACTTGCAGCGGCTAACCGCCTAGAAGCAGCTGAGATGTACAG
  GAAGGCTGCCTGGGAGGCATATCTGAGTAGGCTTGCTGTTCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207718 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEVASQALGPLVTELYDVQAFKFGSFVLKSGLSSPVYIDLRGIVSRPRLLSQVADILFQTAKNAGISFDS
  VCGVPYTALPLATVICSANHIPMLIRRKETKDYGTKRLVEGEINPGQTCLVIEDVVTSGASVLETVEVLQ
  KEGLKVTDAIVLLDREQGGKDKLQAQGIRLHAVCTLSQMLEILQQQEKIDADMVGRVKRFIQENVFSAAN
  HNGLPPPEKKACKELSFGARAELPGTHPLASKLLRLMQKKETNLCLSADVSEARELLQLADALGPSICML
  KTHVDILNDFTLDVMEELTALAKRHEFLIFEDRKFADIGNTVKKQYESGTFKIASWADIVNAHVVPGSGV
  VKGLQEVGLPLHRACLLIAEMSSAGSLATGNYTKAAVGMAEEHCEFVIGFISGSRVSMKPEFLHLTPGVQ
  LETGGDHLGQQYNSPQEVIGKRGSDVIIVGRGILAAANRLEAAEMYRKAAWEAYLSRLAVQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009471
• ORF Size: 1446 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009471.3
• RefSeq Size: 3219 bp
• RefSeq ORF: 1446 bp
• Locus ID: 22247
• UniProt ID: P13439
• Cytogenetics: 16 B3
• MW: 52.3 kDa