Sox10 (BC018551) Mouse Tagged ORF Clone

CAT#: MR207447

Sox10 (Myc-DDK-tagged) - Mouse SRY-box containing gene 10 (cDNA clone MGC:18351 IMAGE:3675437)

Product Images

Circular map for MR207447

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sox10
• Synonyms: Dom; gt; Sox21
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207447 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGAGGAACAAGACCTATCAGAGGTGGAGCTGAGCCCTGTGGGCTCGGAGGAACCCCGCTGCCTGT
  CCCCAGGCAGCGCGCCGTCGCTGGGACCCGACGGCGGCGGCGGTGGCTCGGGCTTGCGAGCCAGCCCGGG
  GCCCGGTGAACTGGGCAAGGTCAAGAAGGAACAGCAGGACGGCGAGGCGGACGATGACAAGTTCCCCGTG
  TGCATCCGCGAGGCGGTCAGCCAGGTGCTCAGCGGCTACGACTGGACGCTGGTGCCCATGCCCGTGCGCG
  TCAACGGTGCCAGCAAGAGCAAGCCGCACGTCAAGAGGCCCATGAACGCCTTCATGGTGTGGGCACAGGC
  GGCACGCAGAAAGCTAGCCGACCAGTACCCTCACCTCCACAATGCTGAGCTCAGCAAGACACTAGGCAAG
  CTCTGGAGGTTGCTGAACGAAAGTGACAAGCGCCCCTTCATTGAGGAGGCTGAGCGGCTCCGGATGCAGC
  ACAAAAAGGACCATCCGGACTACAAGTACCAACCTCGGCGGCGGAAGAACGGGAAGGCAGCCCAGGGGGA
  GGCAGAATGCCCAGGCGGGGAAGCCGAGCAAGGAGGGGCTGCTGCTATTCAGGCTCACTACAAGAGTGCC
  CACCTGGACCACCGGCACCCAGAAGAAGGCTCCCCCATGTCAGATGGGAACCCAGAGCACCCCTCAGGCC
  AGAGCCATGGCCCCCCAACCCCTCCAACCACCCCAAAGACAGAGCTGCAGTCCGGCAAGGCAGACCCCAA
  AAGGGATGGGCGCTCCTTGGGGGAGGGCGGGAAGCCCCACATCGACTTCGGCAACGTGGACATCGGGGAG
  ATCAGCCACGAGGTAATGTCCAACATGGAAACCTTTGATGTGACTGAGCTGGACCAATACCTGCCACCCA
  ACGGGCACCCAGGCCATGTGGGTAGCTACTCGGCAGCTGGCTATGGGCTGGGCAGTGCCCTGGCTGTGGC
  CAGTGGACACTCTGCCTGGATCTCCAAGCCACCAGGTGTGGCTCTGCCCACGGTCTCGCCCCCTGGTGTG
  GATGCCAAAGCCCAGGTGAAGACAGAGACCACAGGCCCCCAGGGACCCCCACACTACACCGACCAGCCGT
  CCACTTCCCAGATCGCCTACACCTCCCTCAGTCTGCCCCACTACGGCTCCGCCTTCCCCTCCATCTCACG
  ACCCCAGTTTGACTATTCTGACCATCAGCCCTCAGGACCCTATTATGGCCATGCAGGCCAGGCCTCTGGC
  CTCTATTCAGCCTTTTCTTACATGGGGCCCTCCCAGCGGCCCCTCTACACTGCCATCTCTGACCCCAGCC
  CCTCGGGGCCCCAATCCCACAGTCCCACACACTGGGAGCAGCCAGTATATACGACTCTATCCCGACCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207447 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAEEQDLSEVELSPVGSEEPRCLSPGSAPSLGPDGGGGGSGLRASPGPGELGKVKKEQQDGEADDDKFPV
  CIREAVSQVLSGYDWTLVPMPVRVNGASKSKPHVKRPMNAFMVWAQAARRKLADQYPHLHNAELSKTLGK
  LWRLLNESDKRPFIEEAERLRMQHKKDHPDYKYQPRRRKNGKAAQGEAECPGGEAEQGGAAAIQAHYKSA
  HLDHRHPEEGSPMSDGNPEHPSGQSHGPPTPPTTPKTELQSGKADPKRDGRSLGEGGKPHIDFGNVDIGE
  ISHEVMSNMETFDVTELDQYLPPNGHPGHVGSYSAAGYGLGSALAVASGHSAWISKPPGVALPTVSPPGV
  DAKAQVKTETTGPQGPPHYTDQPSTSQIAYTSLSLPHYGSAFPSISRPQFDYSDHQPSGPYYGHAGQASG
  LYSAFSYMGPSQRPLYTAISDPSPSGPQSHSPTHWEQPVYTTLSRP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC018551
• ORF Size: 1398 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC018551, AAH18551
• RefSeq Size: 2554 bp
• RefSeq ORF: 1400 bp
• Locus ID: 20665
• Cytogenetics: 15 37.7 cM
• MW: 49.9 kDa
• Gene Summary: Transcription factor that plays a central role in developing and mature glia (PubMed:24204311, PubMed:27532821). Specifically activates expression of myelin genes, during oligodendrocyte (OL) maturation, such as DUSP15 and MYRF, thereby playing a central role in oligodendrocyte maturation and CNS myelination (PubMed:24204311, PubMed:27532821). Once induced, MYRF cooperates with SOX10 to implement the myelination program (PubMed:24204311). Transcriptional activator of MITF, acting synergistically with PAX3 (By similarity).[UniProtKB/Swiss-Prot Function]