Ahcy (NM_016661) Mouse Tagged ORF Clone

CAT#: MR206898

Ahcy (Myc-DDK-tagged) - Mouse S-adenosylhomocysteine hydrolase (Ahcy)

Product Images

Circular map for MR206898

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ahcy
• Synonyms: AA987153; AL024110; CuBP; SAHH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206898 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGATAAACTGCCCTACAAAGTCGCGGACATCGGACTGGCCGCCTGGGGACGGAAGGCTCTGGATA
  TAGCTGAGAATGAGATGCCAGGATTGATGCGCATGCGGGAGATGTACTCAGCCTCCAAGCCACTGAAGGG
  TGCTCGCATTGCTGGCTGCCTGCACATGACCGTGGAGACTGCTGTTCTCATTGAGACTCTCGTGGCCCTG
  GGTGCTGAGGTGCGGTGGTCCAGCTGCAACATCTTCTCTACTCAGGACCATGCAGCGGCTGCCATTGCCA
  AGGCTGGCATTCCAGTGTTTGCCTGGAAGGGCGAGACAGATGAGGAGTACCTGTGGTGCATTGAGCAGAC
  GCTGCACTTCAAGGACGGACCCCTCAACATGATTCTGGATGATGGTGGTGACCTTACTAACCTCATCCAC
  ACCAAATACCCACAGCTTCTGTCAGGCATCCGAGGTATCTCTGAGGAGACCACGACTGGGGTCCACAACC
  TCTACAATATGATGTCCAATGGGATACTGAAGGTGCCTGCCATCAATGTCAACGATTCTGTCACCAAGAG
  CAAGTTTGACAACCTCTATGGCTGCCGGGAGTCCCTCATAGATGGCATCAAACGGGCCACAGATGTGATG
  ATTGCGGGCAAGGTGGCGGTGGTGGCAGGCTATGGTGATGTGGGCAAGGGCTGTGCCCAGGCCCTGAGGG
  GTTTTGGGGCCCGAGTCATCATCACCGAGATCGACCCCATCAATGCACTGCAAGCTGCCATGGAGGGCTA
  TGAGGTAACCACTATGGACGAAGCCTGTAAGGAGGGCAACATCTTTGTCACCACCACAGGCTGTGTGGAT
  ATCATCCTTGGCCGGCACTTTGAGCAGATGAAGGATGACGCCATTGTCTGTAACATTGGACACTTCGATG
  TGGAGATTGATGTGAAGTGGCTCAATGAGAACGCGGTGGAGAAAGTGAACATCAAGCCCCAGGTGGACCG
  CTACTGGCTAAAGAATGGGCGCCGCATCATCTTGCTGGCTGAAGGCCGTCTGGTCAACCTGGGTTGTGCC
  ATGGGACACCCCAGCTTCGTGATGAGCAACTCCTTCACAAACCAGGTGATGGCACAGATTGAGCTGTGGA
  CCCACCCAGATAAATACCCTGTTGGGGTTCACTTCCTGCCTAAGAAGCTGGATGAGGCGGTGGCTGAAGC
  CCACCTGGGCAAGCTGAATGTGAAGCTGACCAAGCTGACTGAGAAGCAGGCCCAGTACCTGGGCATGCCC
  ATCAACGGCCCCTTCAAGCCTGATCACTACCGCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206898 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSDKLPYKVADIGLAAWGRKALDIAENEMPGLMRMREMYSASKPLKGARIAGCLHMTVETAVLIETLVAL
  GAEVRWSSCNIFSTQDHAAAAIAKAGIPVFAWKGETDEEYLWCIEQTLHFKDGPLNMILDDGGDLTNLIH
  TKYPQLLSGIRGISEETTTGVHNLYNMMSNGILKVPAINVNDSVTKSKFDNLYGCRESLIDGIKRATDVM
  IAGKVAVVAGYGDVGKGCAQALRGFGARVIITEIDPINALQAAMEGYEVTTMDEACKEGNIFVTTTGCVD
  IILGRHFEQMKDDAIVCNIGHFDVEIDVKWLNENAVEKVNIKPQVDRYWLKNGRRIILLAEGRLVNLGCA
  MGHPSFVMSNSFTNQVMAQIELWTHPDKYPVGVHFLPKKLDEAVAEAHLGKLNVKLTKLTEKQAQYLGMP
  INGPFKPDHYRY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016661
• ORF Size: 1299 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016661.2, NP_057870.2
• RefSeq Size: 2560 bp
• RefSeq ORF: 1299 bp
• Locus ID: 269378
• UniProt ID: P50247
• Cytogenetics: 2 76.94 cM
• MW: 47.7 kDa
• Gene Summary: Adenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine.[UniProtKB/Swiss-Prot Function]