Suclg1 (NM_019879) Mouse Tagged ORF Clone

CAT#: MR204912

Suclg1 (Myc-DDK-tagged) - Mouse succinate-CoA ligase, GDP-forming, alpha subunit (Suclg1), nuclear gene encoding mitochondrial protein

Product Images

Circular map for MR204912

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Suclg1
• Synonyms: 1500000I01Rik; Sucla1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR204912 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCTCCAGCAGCAGCGGCCTCGCCGCCGCCCGCCTGTTGTCGCGCACCTTCCTCCTGCAACAGAATG
  GGATACGACACGGGTCTTACACAGCCTCTCGGAAACATATCTATATTGATAAAAATACGAAGATTATTTG
  CCAGGGTTTCACAGGCAAACAGGGTACCTTTCACAGCCAGCAGGCTTTGGAGTACGGCACCAAACTCGTG
  GGAGGAACCACTCCAGGGAAGGGTGGCCAGAAGCACCTGGGCTTGCCCGTCTTTAATACTGTGAAGGAAG
  CCAAAGAGAAAACAGGAGCGACGGCTTCTGTCATCTATGTCCCTCCTCCTTTTGCTGCTGCTGCCATTAA
  TGAAGCAATCGACGCGGAGATTCCCTTGGTTGTGTGCATTACGGAAGGTATTCCGCAGCAGGATATGGTG
  CGGGTCAAGCACAGACTGACACGCCAGGGAACGACGAGGCTAATCGGGCCAAACTGCCCCGGCGTCATCA
  ACCCTGGAGAATGCAAAATCGGCATCATGCCTGGCCACATTCACAAGAAGGGAAGAATAGGTATCGTGTC
  CAGGTCCGGTACTCTGACTTACGAAGCAGTTCACCAAACAACCCAAGTCGGATTGGGGCAGTCCTTGTGT
  ATTGGCATTGGAGGTGACCCTTTTAATGGGACTGATTTTATTGATTGCCTTGAAGTCTTCCTGAATGATC
  CAGCCACAGAAGGCATCATACTGATTGGTGAAATTGGTGGTCACGCTGAAGAAAATGCTGCCGCGTTTCT
  GAAGGAGCATAACTCAGGTCCAAAGGCCAAGCCTGTAGTGTCCTTCATTGCTGGTATAACTGCTCCTCCT
  GGCAGAAGGATGGGCCATGCAGGGGCAATTATTGCTGGAGGAAAAGGTGGCGCTAAAGAGAAGATCTCTG
  CTCTTCAGAGTGCTGGGGTGGTTGTCAGCATGTCCCCCGCACAGCTGGGAACTACCATTTATAAGGAGTT
  TGAAAAGAGGAAGATGCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR204912 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVSSSSGLAAARLLSRTFLLQQNGIRHGSYTASRKHIYIDKNTKIICQGFTGKQGTFHSQQALEYGTKLV
  GGTTPGKGGQKHLGLPVFNTVKEAKEKTGATASVIYVPPPFAAAAINEAIDAEIPLVVCITEGIPQQDMV
  RVKHRLTRQGTTRLIGPNCPGVINPGECKIGIMPGHIHKKGRIGIVSRSGTLTYEAVHQTTQVGLGQSLC
  IGIGGDPFNGTDFIDCLEVFLNDPATEGIILIGEIGGHAEENAAAFLKEHNSGPKAKPVVSFIAGITAPP
  GRRMGHAGAIIAGGKGGAKEKISALQSAGVVVSMSPAQLGTTIYKEFEKRKML
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019879
• ORF Size: 1002 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019879.1, NM_019879.2, NP_063932.1
• RefSeq Size: 1407 bp
• RefSeq ORF: 1041 bp
• Locus ID: 56451
• UniProt ID: Q9WUM5
• Cytogenetics: 6 C1
• MW: 35 kDa
• Gene Summary: Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and specificity for either ATP or GTP is provided by different beta subunits.[UniProtKB/Swiss-Prot Function]