Ptgr1 (NM_025968) Mouse Tagged ORF Clone

CAT#: MR204840

Ptgr1 (Myc-DDK-tagged) - Mouse prostaglandin reductase 1 (Ptgr1)

Product Images

Circular map for MR204840

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ptgr1
• Synonyms: 2510002C21Rik; Ltb4dh; Zadh3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR204840 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCAGGCCAAGAGCTGGACCCTGAAGAAGCACTTCGAAGGCTTCCCTACGGACGGTAACTTCGAGT
  TGAAGACGACTGAGCTCCCACCCTTAAATAATGGAGAGGTCCTGCTGGAAGCCCTGTTCCTCTCTGTGGA
  TCCTTACATGAGAGTTGCAGCCAAAAAACTGAAGGAGGGCGACAGGATGATGGGCGAGCAAGTGGCCAGA
  GTCGTGGAAAGTAAAAACTCAGCCTTCCCAAAAGGAACGATTGTAGCGGCTTTATTAGGTTGGACATCAC
  ATTCCATTTCTGATGGGAATGGACTGACAAAGCTGCCTGTAGAGTGGCCGGACAAGTTGCCACTGTCTTT
  GGCACTGGGGACAGTTGGCATGCCAGGCCTCACTGCCTACTTTGGCCTGCTTGACATCTGTGGCGTGAAG
  GGTGGAGAAACCGTGATGGTCAGCGCAGCAGCAGGAGCAGTGGGCTCTGTTGTGGGGCAGATAGCTAAGC
  TCAAGGGCTGCAAAGTTGTTGGTACAGCAGGGTCTGATGAAAAGGTTGCCTATCTTAAGAAGCTTGGATT
  CGACGTGGCCTTTAACTACAAGACAGTAAAGTCACTGGAGGAAGCTTTGAGGACAGCCTCTCCCGATGGT
  TATGATTGCTATTTTGATAATGTAGGTGGAGAGTTTTCAAACGCTGTTATACTCCAGATGAAGACATTTG
  GAAGGATTGCCATCTGTGGTGCCATCTCTCAATACAACCGTACTGGACCATGTCCCCAGGGCCCAGCCCC
  AGAGGTGGTTATCTATCAGCAACTCCGCATGGAGGGGTTCATCGTGAATCGGTGGCAAGGAGAAGTCCGC
  CAGAAGGCTCTGACAGAGTTGATGAATTGGGTCTCAGAGGGTAAAGTCCAGTATCATGAACACGTCACTG
  AAGGATTTGAGAAAATGCCCGCAGCATTTATGGGAATGTTGAAAGGAGAAAATCTGGGGAAGACCATAGT
  GAAAGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR204840 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVQAKSWTLKKHFEGFPTDGNFELKTTELPPLNNGEVLLEALFLSVDPYMRVAAKKLKEGDRMMGEQVAR
  VVESKNSAFPKGTIVAALLGWTSHSISDGNGLTKLPVEWPDKLPLSLALGTVGMPGLTAYFGLLDICGVK
  GGETVMVSAAAGAVGSVVGQIAKLKGCKVVGTAGSDEKVAYLKKLGFDVAFNYKTVKSLEEALRTASPDG
  YDCYFDNVGGEFSNAVILQMKTFGRIAICGAISQYNRTGPCPQGPAPEVVIYQQLRMEGFIVNRWQGEVR
  QKALTELMNWVSEGKVQYHEHVTEGFEKMPAAFMGMLKGENLGKTIVKA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025968
• ORF Size: 990 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025968.1, NM_025968.2, NM_025968.3, NP_080244.1
• RefSeq Size: 2104 bp
• RefSeq ORF: 990 bp
• Locus ID: 67103
• UniProt ID: Q91YR9
• Cytogenetics: 4 B3
• MW: 35.6 kDa
• Gene Summary: Functions as 15-oxo-prostaglandin 13-reductase and acts on 15-oxo-PGE1, 15-oxo-PGE2 and 15-oxo-PGE2-alpha. Has no activity towards PGE1, PGE2 and PGE2-alpha. Catalyzes the conversion of leukotriene B4 into its biologically less active metabolite, 12-oxo-leukotriene B4. This is an initial and key step of metabolic inactivation of leukotriene B4 (By similarity).[UniProtKB/Swiss-Prot Function]