Rfxank (NM_001025589) Mouse Tagged ORF Clone

CAT#: MR203366

Rfxank (Myc-DDK-tagged) - Mouse regulatory factor X-associated ankyrin-containing protein (Rfxank), transcript variant 2

Product Images

Circular map for MR203366

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rfxank
• Synonyms: Tvl1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR203366 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCCACTCAGGTTGCAGAGAACCTTGTCCCAAACCAGCAACCTCCTGTTCCTGACCTAGAGGATC
  CTGAGGACACCAGAGATGAGTCCCCAGAGAACTCAGACACTGTCGTCCTCAGCCTGTTCCCCTGCACCCC
  AGATGCTGTGAATCCTGAAGCAGATGCCAGTGCATCCTCACTGCAGGGAAGTTTCTTGAAGCACTCCACA
  ACCCTCACAAACCGGCAACGTGGGAATGAGGTCTCAGCTCTGCCAGCCACCCTGGACTCCCTTTCTATCC
  ACCAGCTTGCAGCCCAAGGGGAGCTGAGCCAACTGAAGGATCATCTGCGGAAGGGAAACAACCTGATCAA
  CAAACCGGATGAGCGTGGCTTCACCCCCCTCATCTGGGCCTCAGCCTTTGGAGAAATTGAGACAGTTCGC
  TTCCTGCTAGACTGGGGTGCTGACCCCCACATCCTGGCCAAGGAGCGGGAGAGCGCACTGTCACTTGCCA
  GTATGGGTGGCTACACGGACATCGTGAGGTTGCTGCTTGACCGTGACGTGGATATCAACATCTATGACTG
  GAATGGAGGAACACCACTGCTCTATGCTGTGCGTGGGAACCACGTGAAGTGTGTGGAGGCCTTACTGGCC
  CGGGGTGCTGACCTCACCACAGAGGCTGACTCTGGCTACACCCCAATGGATCTCGCAGTGGCCCTGGGAT
  ACCGCAAAGTGCAACAGGTGATGGAGAGCCACATCCTCAGATTGTTCCAGAGCACCCTGGGGCCTGTGGA
  CCCCGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR203366 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEPTQVAENLVPNQQPPVPDLEDPEDTRDESPENSDTVVLSLFPCTPDAVNPEADASASSLQGSFLKHST
  TLTNRQRGNEVSALPATLDSLSIHQLAAQGELSQLKDHLRKGNNLINKPDERGFTPLIWASAFGEIETVR
  FLLDWGADPHILAKERESALSLASMGGYTDIVRLLLDRDVDINIYDWNGGTPLLYAVRGNHVKCVEALLA
  RGADLTTEADSGYTPMDLAVALGYRKVQQVMESHILRLFQSTLGPVDPE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025589
• ORF Size: 780 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025589.1, NP_001020760.1
• RefSeq Size: 3652 bp
• RefSeq ORF: 780 bp
• Locus ID: 19727
• UniProt ID: Q9Z205
• Cytogenetics: 8 B3.3
• MW: 28.3 kDa
• Gene Summary: Activates transcription from class II MHC promoters. Activation requires the activity of the MHC class II transactivator/CIITA. May regulate other genes in the cell. RFX binds the X1 box of MHC-II promoters (By similarity). May also potentiate the activation of RAF1 (PubMed:10329666).[UniProtKB/Swiss-Prot Function]