Rage (BC087925) Mouse Tagged ORF Clone

CAT#: MR202873

Rage (Myc-DDK-tagged) - Mouse renal tumor antigen (cDNA clone MGC:107277 IMAGE:6532951)

Product Images

Circular map for MR202873

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rage
• Synonyms: MGC46883, MOK, RAGE1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR202873 representing BC087925
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAGGAGAAGACACCCATTATCAGAGAAAAAGATTATGCTCTACATGTATCAGCTGTGCAAATCGC
  TGGACCACATGCACAGAAACGGGATCTTTCACAGAGACGTGAAGCCGGAGAATATCCTAGTAAAGCAGGA
  TGTCCTGAAACTAGGGGACTTCGGGTCATGCCGGAGTGTCTATTCCAAGCAGCCCTACACAGAGTACATC
  TCTACCCGGTGGTACCGGGCCCCAGAGTGTCTCCTCACCGACGGGTTCTACACATACAAGATGGACCTGT
  GGAGCGCTGGCTGCGTGTTCTACGAGATTGCCAGGGCAGCTGAGACACAGACCCTGGCCAAACACAGAAG
  AGCTTTCTGTCCCAAGTTCTCCATGGTGCCAGAATCATCCAGTCACAACTGGTCATTCTCACAGGAAGGC
  AGAAAGCAGAAACAGTCCCTGCGGCACGAGGAGGGCCATGCCAGACGACAGGGGCCGACCAGCTTGATGG
  AGCTTCCCAAACTGAGGCTGTCGGGAATGACCAAACTGTCATCATGTTCCAGCCCTGCACTGCGGTCAGT
  GCTGGGCACTGGAGCAAATGGAAAAGTCCCTGTGCTGAGACCCCTGAAGTGTGCTGCTGTGAACAAGAAG
  ACAGACACGCAGAAGGACATCAAACCTCACCTGAAACACTATCACCTGCCCACGATCAACAGGAAAGGGG
  GCGAATAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR202873 representing BC087925
  Red=Cloning site Green=Tags(s)
  
  MKRRRHPLSEKKIMLYMYQLCKSLDHMHRNGIFHRDVKPENILVKQDVLKLGDFGSCRSVYSKQPYTEYI
  STRWYRAPECLLTDGFYTYKMDLWSAGCVFYEIARAAETQTLAKHRRAFCPKFSMVPESSSHNWSFSQEG
  RKQKQSLRHEEGHARRQGPTSLMELPKLRLSGMTKLSSCSSPALRSVLGTGANGKVPVLRPLKCAAVNKK
  TDTQKDIKPHLKHYHLPTINRKGGEY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC087925
• ORF Size: 708 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC087925.1
• RefSeq Size: 1161 bp
• RefSeq ORF: 710 bp
• Locus ID: 26448
• Cytogenetics: 12 F1
• MW: 42.6 kDa
• Gene Summary: Able to phosphorylate several exogenous substrates and to undergo autophosphorylation (PubMed:10421840). Negatively regulates cilium length in a cAMP and mTORC1 signaling-dependent manner (PubMed:25243405).[UniProtKB/Swiss-Prot Function]