Cblb (NM_001033238) Mouse Tagged ORF Clone

CAT#: MG227649

Cblb (tGFP-tagged) - Mouse Casitas B-lineage lymphoma b (Cblb), (10ug)

Product Images

Circular map for MG227649

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cblb
• Synonyms: AI429560; AI851073; Cbl-b
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227649 representing NM_001033238
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAAATTCTATGAATGGCAGAAATCCTGGTGGTCGAGGAGGAAACCCCCGCAAAGGTCGCATTTTGG
  GGATTATTGATGCTATTCAGGATGCAGTCGGACCCCCAAAGCAAGCTGCAGCTGACCGCAGGACTGTGGA
  GAAGACTTGGAAACTCATGGACAAAGTGGTAAGACTGTGCCAAAATCCCAAGCTTCAGTTGAAAAACAGC
  CCACCGTATATACTTGATATTTTACCTGATACGTATCAGCACTTGAGACTTATATTGAGTAAATATGATG
  ACAACCAGAAGCTGGCTCAACTGAGCGAGAATGAGTACTTTAAAATCTACATCGATAGTCTCATGAAGAA
  GTCGAAGCGAGCGATCCGGCTCTTTAAAGAAGGCAAGGAAAGGATGTACGAAGAGCAGTCGCAGGACAGA
  CGGAATCTCACAAAGCTGTCCCTTATCTTCAGTCACATGCTGGCAGAAATCAAGGCGATCTTTCCCAATG
  GCCAGTTCCAGGGAGATAACTTCCGGATCACCAAAGCAGATGCTGCTGAGTTCTGGAGGAAGTTTTTTGG
  AGACAAAACTATTGTACCATGGAAAGTCTTCAGACAGTGCCTGCATGAGGTCCATCAGATCAGCTCTGGC
  CTGGAAGCAATGGCTCTGAAGTCAACCATTGATTTAACTTGCAATGATTACATCTCAGTGTTTGAATTTG
  ATATTTTTACCAGGCTATTTCAGCCCTGGGGCTCTATTTTACGGAATTGGAACTTCTTGGCTGTGACTCA
  CCCGGGATACATGGCATTTCTCACGTATGATGAAGTTAAAGCTCGGCTACAGAAATACAGCACCAAGCCT
  GGAAGTTACATTTTCCGGTTAAGCTGCACTCGGCTGGGACAATGGGCCATTGGCTATGTGACTGGGGACG
  GCAATATCCTACAGACCATACCTCATAACAAGCCCCTGTTCCAAGCCCTGATTGATGGTAGCAGGGAAGG
  CTTTTATCTTTATCCTGATGGGCGGAGCTATAACCCTGATTTAACCGGATTATGTGAACCTACACCTCAC
  GATCATATAAAAGTTACACAGGAGCAGTATGAACTGTATTGTGAAATGGGCTCCACTTTTCAGCTCTGCA
  AGATCTGTGCAGAGAATGACAAAGATGTCAAGATTGAGCCTTGCGGGCATCTGATGTGCACTTCGTGCCT
  TACCGCGTGGCAGGAGTCTGATGGCCAAGGCTGCCCCTTCTGTCGCTGTGAGATAAAAGGAACGGAGCCC
  ATCATTGTGGACCCCTTCGACCCCAGAGATGAAGGCTCCAGGTGCTGCAGCATCATTGACCCTTTCAGCA
  TCCCCATGCTTGACTTGGACGATGACGATGATCGAGAGGAGTCTTTGATGATGAACAGGCTGGCGAGTGT
  TCGAAAGTGCACGGACAGGCAGAACTCACCAGTCACATCGCCAGGCTCCTCACCCCTTGCCCAGAGAAGA
  AAGCCTCAGCCAGACCCTCTCCAGATCCCCCACCTCAGCCTGCCACCAGTGCCTCCCCGCCTAGATCTCA
  TTCAGAAAGGCATCGTGCGCTCTCCGTGTGGCAGCCCCACAGGCTCGCCAAAGTCTTCTCCATGCATGGT
  TAGAAAACAAGACAAACCACTCCCGGCACCCCCTCCTCCCTTGAGAGATCCACCTCCTCCACCAGAGAGG
  CCTCCCCCCATCCCACCTGACAATAGACTGAGCAGACACTTCCACCATGGAGAGAGTGTGCCTTCCCGGG
  ACCAGCCCATGCCCCTCGAAGCCTGGTGCCCTCGGGATGCCTTCGGGACTAACCAGGTGATGGGATGTCG
  CATCCTCGGGGATGGCTCTCCAAAGCCTGGCGTCACCGCAAACTCCAGCTTAAATGGGAGGCACAGTAGA
  ATGGGCTCCGAGCAGGTTCTTATGAGGAAACACAGACGCCATGATTTGCCTTCAGAAGGAGCCAAGGTCT
  TTTCCAATGGCCACCTTGCCACTGAAGAATACGATGTCCCTCCTCGGCTTTCCCCTCCGCCTCCAGTCAC
  CACCCTTCTCCCAAGCATAAAGTGTACTGGTCCATTAGCAAATTGTCTCTCAGAGAAAACTAGAGACACG
  GTAGAAGATGATGACGATGAATACAAGATTCCTTCATCCCATCCTGTGTCCCTGAATTCACAACCATCTC
  ATTGCCATAATGTCAAAGCTCCTGTTCGGTCTTGTGATAATGGTCATTGTATATTGAATGGAACTCATGG
  TGCGCCTTCAGAGATGAAGAAATCGAACATCCCAGATTTAGGCATCTATTTGAAGGGTGAGGACGCCTTT
  GATGCTCTCCCTCCATCCCTCCCTCCTCCCCCACCTCCTGCAAGACACAGTCTCATCGAACATTCAAAAC
  CTCCAGGCTCCAGTAGCCGGCCTTCCTCAGGGCAGGACCTTTTCCTTCTTCCTTCAGATCCCTTTTTTGA
  CCCAACAAGTGGCCAAGTTCCATTGCCTCCTGCCAGGAGAGCAGCAGGAGACAGCGGCAAAGCCAACAGA
  GCCTCGCAGGACTATGACCAGCTCCCTTCATCTTCCGATGGTTCGCAAGCACCAGCTAGACCCCCCAAAC
  CACGACCCCGAAGGACTGCACCAGAAATTCATCACAGAAAGCCCCATGGGCCCGAAGCGGCGTTGGAAAA
  TGTCGATGCAAAAATTGCAAAACTCATGGGAGAGGGGTATGCCTTTGAAGAGGTGAAGAGAGCCTTAGAG
  ATTGCCCAGAATAACGTGGAAGTGGCCAGGAGCATACTTCGAGAATTTGCCTTCCCCCCTCCTGTCTCCC
  CACGTCTGAATCTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227649 representing NM_001033238
  Red=Cloning site Green=Tags(s)
  
  MANSMNGRNPGGRGGNPRKGRILGIIDAIQDAVGPPKQAAADRRTVEKTWKLMDKVVRLCQNPKLQLKNS
  PPYILDILPDTYQHLRLILSKYDDNQKLAQLSENEYFKIYIDSLMKKSKRAIRLFKEGKERMYEEQSQDR
  RNLTKLSLIFSHMLAEIKAIFPNGQFQGDNFRITKADAAEFWRKFFGDKTIVPWKVFRQCLHEVHQISSG
  LEAMALKSTIDLTCNDYISVFEFDIFTRLFQPWGSILRNWNFLAVTHPGYMAFLTYDEVKARLQKYSTKP
  GSYIFRLSCTRLGQWAIGYVTGDGNILQTIPHNKPLFQALIDGSREGFYLYPDGRSYNPDLTGLCEPTPH
  DHIKVTQEQYELYCEMGSTFQLCKICAENDKDVKIEPCGHLMCTSCLTAWQESDGQGCPFCRCEIKGTEP
  IIVDPFDPRDEGSRCCSIIDPFSIPMLDLDDDDDREESLMMNRLASVRKCTDRQNSPVTSPGSSPLAQRR
  KPQPDPLQIPHLSLPPVPPRLDLIQKGIVRSPCGSPTGSPKSSPCMVRKQDKPLPAPPPPLRDPPPPPER
  PPPIPPDNRLSRHFHHGESVPSRDQPMPLEAWCPRDAFGTNQVMGCRILGDGSPKPGVTANSSLNGRHSR
  MGSEQVLMRKHRRHDLPSEGAKVFSNGHLATEEYDVPPRLSPPPPVTTLLPSIKCTGPLANCLSEKTRDT
  VEDDDDEYKIPSSHPVSLNSQPSHCHNVKAPVRSCDNGHCILNGTHGAPSEMKKSNIPDLGIYLKGEDAF
  DALPPSLPPPPPPARHSLIEHSKPPGSSSRPSSGQDLFLLPSDPFFDPTSGQVPLPPARRAAGDSGKANR
  ASQDYDQLPSSSDGSQAPARPPKPRPRRTAPEIHHRKPHGPEAALENVDAKIAKLMGEGYAFEEVKRALE
  IAQNNVEVARSILREFAFPPPVSPRLNL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033238
• ORF Size: 2814 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033238.1, NP_001028410.1
• RefSeq Size: 6323 bp
• RefSeq ORF: 2817 bp
• Locus ID: 208650
• UniProt ID: Q3TTA7
• Cytogenetics: 16 B5
• Gene Summary: E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B-cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. Also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T-cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. Slightly promotes SRC ubiquitination. May be involved in EGFR ubiquitination and internalization. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3. In association with CBL, required for proper feedback inhibition of ciliary platelet-derived growth factor receptor-alpha (PDGFRA) signaling pathway via ubiquitination and internalization of PDGFRA (PubMed:29237719).[UniProtKB/Swiss-Prot Function]