Wnt4 (NM_009523) Mouse Tagged ORF Clone

CAT#: MG226763

Wnt4 (tGFP-tagged) - Mouse wingless-related MMTV integration site 4 (Wnt4), (10ug)

Product Images

Circular map for MG226763

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Wnt4
• Synonyms: Wnt-4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226763 representing NM_009523
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCCCCGTTCGTGCCTGCGGTCCCTGCGACTCCTCGTCTTCGCCGTGTTCTCGGCCGCCGCGAGCA
  ATTGGCTGTACCTGGCCAAGCTGTCATCGGTGGGCAGCATCTCCGAAGAGGAGACGTGCGAGAAGCTCAA
  AGGCCTGATCCAGAGGCAGGTGCAGATGTGCAAACGGAACCTCGAGGTGATGGACTCAGTGCGCCGTGGC
  GCCCAGCTGGCCATCGAGGAGTGCCAATACCAGTTCCGGAATCGGCGCTGGAACTGTTCCACACTGGACT
  CCCTCCCTGTCTTTGGGAAGGTGGTGACACAAGGGACCCGGGAGGCGGCCTTTGTATACGCCATCTCTTC
  AGCAGGTGTGGCCTTTGCAGTGACAAGGGCATGCAGCAGTGGAGAACTGGAGAAGTGTGGCTGTGACCGG
  ACAGTGCACGGGGTCAGCCCACAGGGCTTCCAGTGGTCAGGATGCTCGGACAACATCGCCTATGGCGTAG
  CCTTCTCACAGTCCTTTGTGGACGTCCGGGAGAGGAGCAAGGGGGCCTCCTCCAGCCGGGCACTCATGAA
  TCTTCACAACAACGAGGCTGGCAGGAAGGCCATCTTGACACACATGCGGGTGGAGTGCAAGTGTCACGGG
  GTGTCGGGCTCCTGCGAGGTAAAGACGTGCTGGCGTGCTGTACCGCCCTTCCGCCAGGTTGGCCACGCGC
  TAAAGGAGAAGTTTGACGGTGCCACGGAGGTGGAGCCACGACGCGTAGGCTCCTCCCGGGCGCTGGTGCC
  TCGGAATGCACAGTTCAAGCCACATACAGATGAGGACCTGGTATACCTGGAGCCTAGCCCGGACTTCTGT
  GAGCAGGACATCCGCAGTGGCGTGCTAGGCACGAGGGGCCGCACGTGCAACAAGACATCTAAAGCCATTG
  ACGGCTGCGAGCTACTGTGCTGTGGCCGCGGCTTCCACACAGCGCAAGTGGAGCTGGCCGAGCGCTGTGG
  CTGCAGGTTCCACTGGTGCTGCTTCGTCAAGTGCCGGCAGTGCCAGCGGCTCGTGGAGATGCACACGTGC
  CGGTG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226763 representing NM_009523
  Red=Cloning site Green=Tags(s)
  
  MSPRSCLRSLRLLVFAVFSAAASNWLYLAKLSSVGSISEEETCEKLKGLIQRQVQMCKRNLEVMDSVRRG
  AQLAIEECQYQFRNRRWNCSTLDSLPVFGKVVTQGTREAAFVYAISSAGVAFAVTRACSSGELEKCGCDR
  TVHGVSPQGFQWSGCSDNIAYGVAFSQSFVDVRERSKGASSSRALMNLHNNEAGRKAILTHMRVECKCHG
  VSGSCEVKTCWRAVPPFRQVGHALKEKFDGATEVEPRRVGSSRALVPRNAQFKPHTDEDLVYLEPSPDFC
  EQDIRSGVLGTRGRTCNKTSKAIDGCELLCCGRGFHTAQVELAERCGCRFHWCCFVKCRQCQRLVEMHTC
  R
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009523
• ORF Size: 1053 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009523.1, NP_033549.1
• RefSeq Size: 1101 bp
• RefSeq ORF: 1056 bp
• Locus ID: 22417
• UniProt ID: P22724
• Cytogenetics: 4 D3
• Gene Summary: Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Plays an important role in the embryonic development of the urogenital tract and the lung (PubMed:7990960, PubMed:9989404, PubMed:16054034, PubMed:17537789, PubMed:19830824, PubMed:26321050). Required for normal mesenchyme to epithelium transition during embryonic kidney development (PubMed:7990960, PubMed:16054034, PubMed:17537789, PubMed:19830824). Required for the formation of early epithelial renal vesicles during kidney development (PubMed:16054034). Required for normal formation of the Mullerian duct in females, and normal levels of oocytes in the ovaries (PubMed:9989404, PubMed:19830824). Required for normal down-regulation of 3 beta-hydroxysteroid dehydrogenase in the ovary (PubMed:9989404). Required for normal lung development and for normal patterning of trachael cartilage rings (PubMed:26321050).[UniProtKB/Swiss-Prot Function]