Bmp6 (NM_007556) Mouse Tagged ORF Clone

CAT#: MG225853

Bmp6 (tGFP-tagged) - Mouse bone morphogenetic protein 6 (Bmp6), (10ug)

Product Images

Circular map for MG225853

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Bmp6
• Synonyms: D13Wsu115; D13Wsu115e; Vg; Vgr1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225853 representing NM_007556
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCGGGCTGGGGCGGAGGGCGCAGTGGCTGTGCTGGTGGTGGGGGTTGCTGTGCAGCTGCGGCCCCC
  CGCCACTGCGGCCCCCTCTGCCGGTAGCCGCGGCCGCCGCCGGGGGGCAGCTGCTGGGAGCCGGCGGGAG
  CCCGGTGCGCGCTGAGCAGCCACCGCCACAGTCCTCTTCTTCGGGCTTCCTCTATCGGCGGCTCAAGACC
  CACGAGAAGCGGGAGATGCAAAAGGAGATCCTGTCGGTGCTGGGGCTCCCGCACAGGCCGCGGCCCCTGC
  ACGGTCTCCAGCAGCCTCAGCCCCCGGTGCTCCCGCCACAGCAGCAGCAGCAGCAGCAGCAGCAGCAGAC
  GGCCCGCGAGGAGCCCCCTCCAGGGCGGCTGAAGTCCGCTCCACTCTTCATGCTGGATCTCTACAACGCC
  CTGTCCAATGACGACGAAGAGGATGGGGCATCGGAGGGTGTGGGGCAAGAGCCTGGGTCCCACGGAGGGG
  CCAGCTCGTCCCAGCTCAGGCAGCCGTCTCCCGGCGCTGCACACTCCTTGAACCGCAAGAGTCTCCTGGC
  CCCGGGACCCGGTGGCGGTGCGTCCCCACTGACTAGCGCGCAGGACAGCGCTTTCCTCAACGACGCGGAC
  ATGGTCATGAGCTTTGTGAACCTGGTGGAGTACGACAAGGAGTTCTCCCCACATCAACGACACCACAAAG
  AGTTCAAGTTCAACCTATCCCAGATTCCTGAGGGTGAGGCGGTGACGGCTGCTGAGTTCCGCGTCTACAA
  GGACTGTGTGGTGGGGAGTTTTAAAAACCAAACCTTTCTTATCAGCATTTACCAAGTCTTGCAGGAGCAT
  CAGCACAGAGACTCTGACCTATTTTTGTTGGACACCCGGGTGGTGTGGGCCTCAGAAGAAGGTTGGCTGG
  AATTTGACATCACAGCAACTAGCAATCTGTGGGTGGTGACACCGCAGCACAACATGGGGCTCCAGCTGAG
  TGTGGTGACTCGGGATGGACTCCACGTCAACCCCCGTGCGGCGGGCCTGGTGGGCAGAGACGGCCCTTAC
  GACAAGCAGCCCTTCATGGTGGCCTTCTTCAAGGTGAGCGAGGTCCACGTGCGCACCACCAGGTCAGCCT
  CCAGTCGGCGGCGGCAGCAGAGTCGCAACCGGTCCACCCAGTCGCAGGACGTGTCCCGGGGCTCCGGTTC
  TTCAGACTACAACGGCAGTGAGTTAAAAACAGCTTGCAAGAAGCATGAGCTCTATGTGAGCTTCCAGGAC
  CTGGGATGGCAGGACTGGATCATTGCACCCAAAGGCTACGCTGCCAACTACTGTGATGGAGAGTGTTCCT
  TCCCACTCAACGCACACATGAATGCCACCAACCACGCCATTGTACAGACCTTGGTCCACCTTATGAATCC
  CGAGTACGTCCCCAAACCATGCTGCGCACCAACCAAACTGAATGCCATCTCGGTTCTTTACTTCGATGAT
  AACTCCAATGTCATCTTGAAAAAGTACAGGAATATGGTCGTGAGAGCTTGTGGTTGCCAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225853 representing NM_007556
  Red=Cloning site Green=Tags(s)
  
  MPGLGRRAQWLCWWWGLLCSCGPPPLRPPLPVAAAAAGGQLLGAGGSPVRAEQPPPQSSSSGFLYRRLKT
  HEKREMQKEILSVLGLPHRPRPLHGLQQPQPPVLPPQQQQQQQQQQTAREEPPPGRLKSAPLFMLDLYNA
  LSNDDEEDGASEGVGQEPGSHGGASSSQLRQPSPGAAHSLNRKSLLAPGPGGGASPLTSAQDSAFLNDAD
  MVMSFVNLVEYDKEFSPHQRHHKEFKFNLSQIPEGEAVTAAEFRVYKDCVVGSFKNQTFLISIYQVLQEH
  QHRDSDLFLLDTRVVWASEEGWLEFDITATSNLWVVTPQHNMGLQLSVVTRDGLHVNPRAAGLVGRDGPY
  DKQPFMVAFFKVSEVHVRTTRSASSRRRQQSRNRSTQSQDVSRGSGSSDYNGSELKTACKKHELYVSFQD
  LGWQDWIIAPKGYAANYCDGECSFPLNAHMNATNHAIVQTLVHLMNPEYVPKPCCAPTKLNAISVLYFDD
  NSNVILKKYRNMVVRACGCH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007556
• ORF Size: 1530 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007556.3
• RefSeq Size: 2410 bp
• RefSeq ORF: 1533 bp
• Locus ID: 12161
• UniProt ID: P20722
• Cytogenetics: 13 18.15 cM
• Gene Summary: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates a wide range of biological processes including iron homeostasis, fat and bone development, and ovulation. Mice lacking this gene exhibit delayed ossification of the sternum, iron overload, and reduced fertility in females. [provided by RefSeq, Jul 2016]