Per1 (NM_011065) Mouse Tagged ORF Clone

CAT#: MG225742

Per1 (tGFP-tagged) - Mouse period homolog 1 (Drosophila) (Per1) transcript variant 1, (10ug)

Product Images

Circular map for MG225742

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Per1
• Synonyms: Hftm; m-rigui; mPer1; Per
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225742 representing NM_011065
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGGTCCCCTAGAAGGGGCCGATGGGGGAGGAGACCCCAGGCCCGGAGAACCTTTTTGTCCTGGAG
  GAGTCCCATCCCCTGGGGCCCCGCAGCACCGGCCTTGTCCAGGCCCCAGCCTGGCTGATGACACTGATGC
  AAACAGCAATGGCTCAAGTGGCAATGAGTCCAACGGACCCGAGTCCAGGGGCGCATCTCAGCGGAGTTCT
  CATAGTTCCTCTTCTGGCAATGGCAAGGACTCAGCTCTGCTGGAGACCACTGAGAGCAGCAAGAGTACAA
  ACTCACAGAGCCCATCCCCACCCAGCAGCTCCATTGCCTACAGCCTCCTGAGTGCGAGCTCAGAGCAGGA
  CAACCCATCTACCAGTGGCTGCAGCAGTGAACAGTCAGCTCGAGCCAGGACCCAGAAAGAACTCATGACT
  GCACTTCGGGAGCTCAAACTTCGACTGCCACCAGAGCGTCGGGGCAAGGGCCGCTCTGGGACCTTGGCCA
  CACTGCAGTACGCTCTGGCCTGTGTCAAGCAGGTTCAGGCTAACCAGGAATATTACCAGCAGTGGAGTCT
  GGAGGAGGGTGAGCCTTGTGCCATGGACATGTCTACTTACACCCTGGAGGAATTGGAGCATATCACATCC
  GAATACACACTTCGAAACCAGGACACCTTCTCTGTGGCTGTGTCCTTCCTGACAGGCCGGATTGTCTATA
  TTTCGGAGCAGGCAGGTGTCCTGCTGCGTTGCAAACGGGATGTGTTTCGGGGTGCCCGCTTCTCAGAGCT
  CCTGGCTCCCCAGGATGTGGGTGTCTTCTATGGCTCTACTACACCATCTCGACTGCCCACCTGGGGCACT
  GGCACCTCTGCAGGTTCAGGTCTCAAGGACTTCACCCAGGAAAAGTCTGTCTTCTGCCGAATCAGAGGAG
  GTCCTGACCGGGATCCAGGGCCTCGGTACCAGCCATTCCGCCTAACCCCATATGTGACCAAGATTCGGGT
  CTCAGATGGAGCCCCTGCACAGCCGTGCTGCCTACTCATTGCCGAGCGCATCCACTCTGGTTATGAAGCT
  CCCCGGATCCCTCCTGACAAGAGGATCTTCACCACCCGACACACACCAAGCTGCCTCTTCCAGGATGTAG
  ATGAAAGGGCTGCCCCACTGCTGGGTTACCTTCCCCAGGATCTCCTGGGGGCTCCAGTACTTCTCTTTCT
  ACATCCTGAGGACCGACCCCTCATGCTGGCCATTCATAAGAAGATACTGCAGCTGGCAGGCCAGCCCTTT
  GACCATTCCCCTATTCGCTTCTGTGCTCGGAACGGGGAATATGTCACCATGGACACCAGCTGGGCCGGTT
  TTGTGCACCCCTGGAGCCGCAAGGTGGCTTTCGTGTTGGGTCGCCATAAAGTGCGCACGGCACCCCTGAA
  TGAGGACGTCTTCACTCCCCCAGCCCCCAGCCCAGCTCCGTCCCTGGACTCTGATATCCAGGAGCTCTCA
  GAGCAGATCCATCGATTGCTGCTGCAGCCTGTGCACAGCTCCAGCCCCACGGGGCTCTGTGGAGTTGGCC
  CTCTGATGTCCCCTGGTCCTCTACACAGCCCTGGCTCCTCCAGTGATAGCAATGGGGGGGACGCTGAGGG
  GCCTGGGCCTCCTGCTCCAGTGACTTTCCAGCAGATCTGTAAGGATGTGCATCTGGTAAAGCACCAGGGA
  CAACAGCTCTTCATTGAATCTCGGGCCAAGCCCCCACCCCGGCCCCGCCTCCTTGCTACAGGTACATTCA
  AAGCCAAAGTCCTTCCCTGCCAGTCCCCAAACCCCGAACTGGAGGTGGCCCCAGTTCCTGACCAAGCCTC
  GTTAGCCTTGGCCCCTGAGGAGCCAGAGAGGAAAGAAACCTCTGGCTGTTCCTACCAGCAGATCAACTGC
  CTGGACAGCATCCTCAGGTATTTGGAGAGCTGCAACATTCCCAGTACAACCAAGCGTAAATGTGCCTCCT
  CCTCCTCCTACACTGCCTCTTCAGCCTCTGATGATGACAAGCAGAGGGCAGGTCCAGTTCCTGTGGGGGC
  CAAGAAAGATCCGTCGTCAGCAATGCTGTCTGGGGAGGGGGCAACTCCTCGGAAGGAGCCAGTGGTGGGA
  GGCACCCTGAGCCCGCTCGCCCTGGCCAATAAGGCAGAGAGCGTGGTGTCCGTCACCAGTCAGTGTAGCT
  TCAGCTCCACCATCGTCCATGTGGGAGACAAGAAGCCCCCGGAGTCGGACATCATCATGATGGAAGACCT
  GCCTGGCCTGGCCCCTGGCCCAGCCCCCAGTCCGGCCCCCAGCCCCACAGTAGCCCCTGACCCAACCCCA
  GATGCTTATCGCCCAGTGGGTCTGACCAAGGCCGTGCTGTCCCTGCACACACAGAAGGAAGAGCAAGCCT
  TCCTCAACCGCTTCAGAGATCTTGGCAGGCTTCGTGGACTTGACACCTCTTCTGTGGCCCCCTCAGCCCC
  TGGCTGCCACCATGGCCCCATTCCCCCTGGTCGCCGACACCACTGCCGATCTAAAGCAAAGCGTTCCCGC
  CACCACCACCACCAGACCCCCCGGCCCGAAACTCCCTGCTATGTCTCCCATCCTTCACCTGTGCCCTCTT
  CTGGACCCTGGCCACCCCCACCAGCCACGACCCCCTTCCCAGCAATGGTCCAGCCCTACCCACTCCCAGT
  ATTCTCCCCTCGAGGAGGACCCCAGCCCCTTCCCCCTGCCCCTACATCTGTGTCCCCTGCTACCTTCCCT
  TCTCCCTTAGTGACCCCAATGGTGGCCTTGGTGCTCCCTAACTATCTATTCCCTACCCCACCTAGTTATC
  CATATGGGGTGTCCCAGGCCCCTGTTGAGGGGCCACCCACGCCTGCTTCCCACTCGCCCTCTCCATCCCT
  GCCCCCACCACCTCTCAGCCCCCCCCACCGCCCAGACTCCCCACTGTTCAACTCGAGATGCAGCTCCCCA
  CTCCAGCTCAATCTGCTGCAGCTTGAGGAGTCCCCCCGCACGGAGGGGGGCGCTGCTGCAGGAGGCCCAG
  GAAGCAGTGCTGGGCCCCTGCCTCCCAGTGAGGAGACTGCTGAGCCAGAGGCCAGATTGGTGGAGGTTAC
  TGAGTCGTCCAATCAGGATGCACTTTCAGGCTCCAGCGACCTGCTGGAGCTACTGCTCCAAGAAGACTCT
  CGCTCGGGCACAGGCTCCGCAGCCTCAGGCTCCCTGGGCTCTGGCCTGGGCTCTGGGTCTGGTTCAGGAT
  CCCACGAAGGGGGAAGCACCTCAGCCAGCATCACCCGCAGCAGTCAGAGCAGCCATACAAGCAAGTACTT
  TGGCAGCATCGACTCTTCCGAGGCTGAAGCTGGGGCTGCTCGGGCCAGGACTGAGCCTGGGGACCAGGTC
  ATTAAGTGTGTGCTCCAGGACCCCATCTGGCTGCTCATGGCCAATGCCGACCAGCGTGTCATGATGACAT
  ACCAGGTGCCGTCCAGGGATGCAGCCTCTGTGCTGAAGCAAGACCGGGAGAGGCTCCGGGCCATGCAGAA
  ACAGCAGCCACGGTTCTCAGAGGACCAGAGGCGGGAACTGGGTGCTGTGCACTCCTGGGTCCGGAAGGGC
  CAGCTGCCTCGGGCCCTTGATGTGACGGCGTGTGTGGACTGTGGCAGCAGCGTTCAAGATCCTGGCCACT
  CTGATGACCCCCTCTTCTCAGAACTGGATGGATTGGGGCTGGAGCCCATGGAAGAGGGTGGAGGCGAGGG
  TGGTGGGTGTGGTGTTGGCGGTGGTGGGGGTGATGGTGGTGAGGAGGCCCAGACCCAAATTGGGGCTAAG
  GGTTCAAGCTCTCAGGACTCTGCCATGGAGGAAGAAGAGCAAGGTGGGGGCTCATCCAGCCCAGCTTTAC
  CTGCAGAAGAAAACAGCACCAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225742 representing NM_011065
  Red=Cloning site Green=Tags(s)
  
  MSGPLEGADGGGDPRPGEPFCPGGVPSPGAPQHRPCPGPSLADDTDANSNGSSGNESNGPESRGASQRSS
  HSSSSGNGKDSALLETTESSKSTNSQSPSPPSSSIAYSLLSASSEQDNPSTSGCSSEQSARARTQKELMT
  ALRELKLRLPPERRGKGRSGTLATLQYALACVKQVQANQEYYQQWSLEEGEPCAMDMSTYTLEELEHITS
  EYTLRNQDTFSVAVSFLTGRIVYISEQAGVLLRCKRDVFRGARFSELLAPQDVGVFYGSTTPSRLPTWGT
  GTSAGSGLKDFTQEKSVFCRIRGGPDRDPGPRYQPFRLTPYVTKIRVSDGAPAQPCCLLIAERIHSGYEA
  PRIPPDKRIFTTRHTPSCLFQDVDERAAPLLGYLPQDLLGAPVLLFLHPEDRPLMLAIHKKILQLAGQPF
  DHSPIRFCARNGEYVTMDTSWAGFVHPWSRKVAFVLGRHKVRTAPLNEDVFTPPAPSPAPSLDSDIQELS
  EQIHRLLLQPVHSSSPTGLCGVGPLMSPGPLHSPGSSSDSNGGDAEGPGPPAPVTFQQICKDVHLVKHQG
  QQLFIESRAKPPPRPRLLATGTFKAKVLPCQSPNPELEVAPVPDQASLALAPEEPERKETSGCSYQQINC
  LDSILRYLESCNIPSTTKRKCASSSSYTASSASDDDKQRAGPVPVGAKKDPSSAMLSGEGATPRKEPVVG
  GTLSPLALANKAESVVSVTSQCSFSSTIVHVGDKKPPESDIIMMEDLPGLAPGPAPSPAPSPTVAPDPTP
  DAYRPVGLTKAVLSLHTQKEEQAFLNRFRDLGRLRGLDTSSVAPSAPGCHHGPIPPGRRHHCRSKAKRSR
  HHHHQTPRPETPCYVSHPSPVPSSGPWPPPPATTPFPAMVQPYPLPVFSPRGGPQPLPPAPTSVSPATFP
  SPLVTPMVALVLPNYLFPTPPSYPYGVSQAPVEGPPTPASHSPSPSLPPPPLSPPHRPDSPLFNSRCSSP
  LQLNLLQLEESPRTEGGAAAGGPGSSAGPLPPSEETAEPEARLVEVTESSNQDALSGSSDLLELLLQEDS
  RSGTGSAASGSLGSGLGSGSGSGSHEGGSTSASITRSSQSSHTSKYFGSIDSSEAEAGAARARTEPGDQV
  IKCVLQDPIWLLMANADQRVMMTYQVPSRDAASVLKQDRERLRAMQKQQPRFSEDQRRELGAVHSWVRKG
  QLPRALDVTACVDCGSSVQDPGHSDDPLFSELDGLGLEPMEEGGGEGGGCGVGGGGGDGGEEAQTQIGAK
  GSSSQDSAMEEEEQGGGSSSPALPAEENSTS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011065
• ORF Size: 3873 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011065.5
• RefSeq Size: 4663 bp
• RefSeq ORF: 3876 bp
• Locus ID: 18626
• UniProt ID: O35973
• Cytogenetics: 11 B3
• Gene Summary: This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. This gene is upregulated by Clock/Arntl heterodimers but then represses this upregulation in a feedback loop using Per/Cry heterodimers to interact with Clock/Arntl. Polymorphisms in this gene may increase the risk of getting certain cancers. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jan 2014]