Sp1 (NM_013672) Mouse Tagged ORF Clone

CAT#: MG225692

Sp1 (tGFP-tagged) - Mouse trans-acting transcription factor 1 (Sp1), (10ug)

Product Images

Circular map for MG225692

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Sp1
• Synonyms: 1110003E12Rik; AA450830; AI845540; Sp1-1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225692 representing NM_013672
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGACCAAGATCACTCCATGGATGAAGTGACAGCTGTGGTGAAGATTGAAAAAGATGTTGGTGGCA
  ATAATGGGGGTAGCGGCAATGGTGGCGGTGCCGCCTTTTCTCAGACTCGAAGCAGCAGCACAGGCAGTAG
  CAGCAGCAGTGGTGGCGGAGGAGGGCAGGAATCCCAGCCATCTCCTTTGGCTCTGCTGGCAGCAACCTGC
  AGCAGAATTGAGTCACCCAATGAGAACAGCAACAACTCCCAGGGTCCGAGTCAGTCAGGGGGCACAGGTG
  AACTTGACCTCACAGCCGCACAACTTTCACAGGGTGCCAATGGCTGGCAGATCATCTCTTCCTCCTCTGG
  GGCTACCCCTACCTCAAAGGAACAGAGTGGCAACAGTACCAATGGCAGCGAGTCTTCCAAGAACCGCACA
  GTCTCTGGTGGGCAGTATGTTGTGGCTGCTACCCCCAACTTACAGAACCAGCAAGTTCTGACAGGTCTCC
  CTGGAGTAATGCCTAATATTCAGTATCAAGTAATCCCACAGTTCCAGACTGTTGATGGGCAGCAGCTGCA
  GTTTGCTGCCACTGGGGCCCAAGTGCAGCAGGATGGTTCTGGTCAAATACAGATCATACCAGGTGCAAAC
  CAACAGATCATCCCAAATAGAGGAAGTGGGGGCAACATTATTGCTGCTATGCCAAATCTACTCCAGCAGG
  CTGTCCCCCTTCAAGGCCTTGCTAATAATGTGCTCTCAGGACAGACTCAGTATGTGACCAATGTACCAGT
  GGCCCTGAATGGGAACATCACCTTGCTACCTGTCAACAGCGTTTCTGCAGCTACCCTGACTCCCAGCTCT
  CAGGCAGGCACTATCAGCAGCTCTGGATCCCAGGAGAGCAGCTCACAGCCTGTCACCTCAGGGACTGCCA
  TCAGTTCTGCCAGCTTGGTGTCATCACAAGCTAGTTCCAGCTCCTTTTTTACCAATGCCAATAGTTATTC
  AACAACTACTACCACCAGCAACATGGGAATTATGAACTTTACCAGCAGTGGCTCATCAGGGACTAGTTCT
  CAAGGCCAGACGCCCCAGAGGGTTGGTGGGCTACAAGGGTCTGATTCTCTGAACATCCAGCAGAACCAGA
  CATCAGGAGGCTCGCTGCAAGGAAGTCAGCAGAAAGAGGGAGAGCAAAGTCAGCAGACACAGCAACAACA
  AATCCTTATTCAGCCTCAGCTAGTTCAAGGAGGACAAGCTCTTCAGGCCCTTCAAGCAGCACCATTGTCC
  GGACAGACCTTCACAACTCAAGCTATTTCCCAGGAAACCCTTCAGAACCTCCAGCTTCAGGCTGTTCAAA
  ACTCTGGTCCCATCATCATTCGGACACCAACCGTGGGGCCCAATGGACAGGTCAGTTGGCAGACCCTTCA
  GCTGCAGAATCTTCAAGTTCAGAACCCACAAGCCCAGACAATCACCTTGGCCCCTATGCAGGGTGTTTCC
  TTGGGGCAGACTAGCAGCAGTAATACCACCCTAACACCCATTGCCTCAGCTGCCTCCATTCCTGCTGGCA
  CAGTCACTGTGAATGCTGCTCAACTCTCCTCCATGCCAGGCCTCCAGACCATTAACCTCAGTGCATTGGG
  TACTTCAGGGATCCAGGTGCACCAGCTTCCAGGCCTGCCTTTGGCTATAGCAAACACCCCAGGTGATCAT
  GGAACTCAACTTGGTCTTCATGGATCTGGTGGTGATGGGATACATGATGAGACAGCAGGTGGAGAAGGAG
  AGAATAGCTCTGATCTCCAACCCCAAGCTGGACGCAGGACTCGTCGGGAAGCATGTACATGCCCCTATTG
  CAAAGACAGTGAGGGAAGAGCCTCAGGAGATCCTGGCAAAAAGAAACAGCACATTTGTCACATCCAAGGA
  TGCGGCAAAGTATATGGCAAGACCTCACATCTCCGAGCACACTTGCGCTGGCATACAGGGGAGAGGCCAT
  TCATGTGTAATTGGTCATATTGTGGGAAGCGCTTTACACGTTCGGACGAGCTTCAGAGACATAAACGTAC
  ACATACAGGAGAGAAGAAATTTGCCTGCCCTGAGTGCCCTAAGCGTTTCATGAGGAGTGATCACCTGTCA
  AAGCATATCAAGACTCACCAGAACAAGAAGGGAGGCCCAGGTGTAGCCCTGAGTGTGGGCACATTGCCCC
  TGGACAGTGGGGCAGGTTCAGAAGGCACTGCCACTCCTTCAGCCCTTATTACCACCAATATGGTAGCCAT
  GGAGGCCATCTGTCCAGAGGGTATTGCCCGTCTTGCCAACAGTGGCATCAACGTCATGCAGGTGACAGAG
  CTGCAGTCCATTAATATCAGTGGCAATGGTTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225692 representing NM_013672
  Red=Cloning site Green=Tags(s)
  
  MSDQDHSMDEVTAVVKIEKDVGGNNGGSGNGGGAAFSQTRSSSTGSSSSSGGGGGQESQPSPLALLAATC
  SRIESPNENSNNSQGPSQSGGTGELDLTAAQLSQGANGWQIISSSSGATPTSKEQSGNSTNGSESSKNRT
  VSGGQYVVAATPNLQNQQVLTGLPGVMPNIQYQVIPQFQTVDGQQLQFAATGAQVQQDGSGQIQIIPGAN
  QQIIPNRGSGGNIIAAMPNLLQQAVPLQGLANNVLSGQTQYVTNVPVALNGNITLLPVNSVSAATLTPSS
  QAGTISSSGSQESSSQPVTSGTAISSASLVSSQASSSSFFTNANSYSTTTTTSNMGIMNFTSSGSSGTSS
  QGQTPQRVGGLQGSDSLNIQQNQTSGGSLQGSQQKEGEQSQQTQQQQILIQPQLVQGGQALQALQAAPLS
  GQTFTTQAISQETLQNLQLQAVQNSGPIIIRTPTVGPNGQVSWQTLQLQNLQVQNPQAQTITLAPMQGVS
  LGQTSSSNTTLTPIASAASIPAGTVTVNAAQLSSMPGLQTINLSALGTSGIQVHQLPGLPLAIANTPGDH
  GTQLGLHGSGGDGIHDETAGGEGENSSDLQPQAGRRTRREACTCPYCKDSEGRASGDPGKKKQHICHIQG
  CGKVYGKTSHLRAHLRWHTGERPFMCNWSYCGKRFTRSDELQRHKRTHTGEKKFACPECPKRFMRSDHLS
  KHIKTHQNKKGGPGVALSVGTLPLDSGAGSEGTATPSALITTNMVAMEAICPEGIARLANSGINVMQVTE
  LQSINISGNGF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013672
• ORF Size: 2343 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013672.2, NP_038700.2
• RefSeq Size: 7818 bp
• RefSeq ORF: 2346 bp
• Locus ID: 20683
• UniProt ID: O89090
• Cytogenetics: 15 F3
• Gene Summary: Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter Plays an essential role in the regulation of FE65 gene expression (By similarity). Positively regulates the transcription of the core clock component ARNTL/BMAL1 (PubMed:24030830). Plays a role in protecting cells against oxidative stress following brain injury by regulating the expression of RNF112 (PubMed:27918959).[UniProtKB/Swiss-Prot Function]