Adcy5 (NM_001012765) Mouse Tagged ORF Clone

CAT#: MG225278

Adcy5 (tGFP-tagged) - Mouse adenylate cyclase 5 (Adcy5), (10ug)

Product Images

Circular map for MG225278

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Adcy5
• Synonyms: Ac5; AW121902
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225278 representing NM_001012765
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGCGCCAGATCTCAAGCTTAACTAGCTAGCGGACCGAC
  
  ATGTCCGGCTCCAAAAGCGTGAGCCCCCCGGGCTACGCTGCACAGACAGCGGCGTCGCCAGCGCCCCGGG
  GAGGCCCGGAGCATCGCGCCGCTTGGGGAGAAGCCGATTCCCGCGCCAATGGCTACCCCCACGCCCCCGG
  GGGATCAACCCGCGGCTCCACCAAGAGATCTGGGGGAGCGGTGACCCCACAACAGCAGCAGCGCCTGGCC
  AGCCGTTGGCGCGGTGGCGATGACGACGAAGACCCTCCACTAAGCGGTGATGACCCTCTGGCTGGGGGCT
  TCGGCTTCAGCTTCCGCTCTAAGTCCGCCTGGCAGGAGCGCGGTGGCGACGACGGCGGTCGCGGCAGCAG
  GCGGCAGCGGCGGGGCGCGGCTGGAGGGGGCAGCACCCGGGCGCCCCCTGCGGGCGGCAGCGGCAGTTCG
  GCGGCGGCCGCAGCGGCCGCAGGTGGCACAGAGGTGCGCCCCCGCTCGGTGGAGCTGGGCCTGGAGGAGC
  GTCGAGGAAAAGGCCGAGCGGCCGAGGAGCTGGAGCCCGGGACTGGCATCGTCGAGGATGGAGACGGGTC
  GGAGGATGGAGGCAGTTCTGTGGCGTCAGGCTCTGGGACCGGCGCGGTGCTGTCGTTGGGCGCCTGCTGC
  CTGGCCTTGCTGCAGATATTCCGCTCTAAGAAGTTCCCGTCGGACAAACTGGAGCGTCTGTACCAGCGCT
  ACTTCTTCCGCCTGAACCAGAGCAGTCTCACCATGCTCATGGCCGTGCTGGTGCTTGTGTGCCTGGTCAT
  GCTGGCTTTCCACGCGGCGCGCCCCCCGCTCCAGATAGCCTACCTGGCCGTGTTGGCAGCTGCTGTGGGC
  GTGATCCTTATCATGGCCGTGCTCTGCAACCGTGCAGCGTTCCACCAGGACCACATGGGCCTGGCCTGCT
  ATGCGCTCATTGCAGTGGTGCTGGCCGTCCAGGTAGTGGGCCTGTTGCTGCCACAGCCACGCAGCGCCTC
  CGAGGGCATCTGGTGGACCGTGTTCTTCATCTATACCATCTACACCCTGCTGCCTGTGCGCATGAGGGCT
  GCGGTGCTCAGCGGGGTGCTTCTGTCGGCTCTCCACTTGGCCATCTCTCTGCACACCAACTCCCAGGACC
  AGTTTCTGCTGAAACAGCTTGTCTCCAATGTCCTCATCTTCTCCTGCACCAACATTGTGGGTGTGTGCAC
  TCACTACCCAGCTGAGGTCTCCCAGAGACAGGCCTTCCAGGAGACCCGGGAGTGTATCCAGGCTCGGCTC
  CATTCCCAGCGGGAGAACCAGCAACAGGAGCGTCTCCTGCTGTCTGTCCTTCCCCGTCATGTTGCCATGG
  AGATGAAAGCAGACATCAACGCCAAGCAGGAGGATATGATGTTCCACAAGATCTACATCCAGAAGCATGA
  CAATGTGAGCATCCTGTTTGCTGACATCGAGGGCTTCACCAGCCTGGCTTCCCAGTGTACTGCCCAAGAA
  CTGGTCATGACCCTCAATGAGCTCTTCGCCCGCTTTGACAAGTTGGCTGCGGAGAATCACTGTTTACGGA
  TTAAGATCCTCGGGGATTGTTACTACTGCGTCTCGGGGCTGCCTGAAGCCAGAGCCGACCATGCCCACTG
  CTGCGTGGAGATGGGAATGGACATGATCGAGGCCATCTCGTTGGTCCGGGAGGTGACAGGGGTGAACGTG
  AACATGCGCGTGGGAATTCACAGCGGGAGAGTACACTGCGGTGTCCTTGGTCTCAGAAAGTGGCAATTCG
  ACGTGTGGTCTAACGATGTCACTCTGGCCAACCACATGGAAGCTGGTGGCAAGGCGGGCCGCATCCACAT
  CACCAAGGCCACACTCAACTACCTGAATGGGGACTATGAGGTGGAACCAGGCTGTGGTGGCGATCGCAAT
  GCCTACCTCAAGGAGCACAGCATTGAGACCTTCCTCATCCTGAGCTGTACCCAGAAGCGGAAAGAAGAGA
  AGGCCATGATCGCCAAGATGAACCGCCAGAGAACCAACTCCATCGGACACAATCCGCCTCACTGGGGAGC
  CGAGCGCCCCTTCTACAACCACTTGGGCGGCAACCAGGTGTCAAAGGAGATGAAGAGGATGGGCTTTGAG
  GACCCCAAGGACAAGAATGCCCAGGAAAGTGCGAACCCAGAGGATGAAGTGGACGAGTTTCTGGGTCGGG
  CCATCGATGCCAGGAGCATCGACAGACTGCGATCTGAACACGTCCGCAAGTTCCTCCTGACCTTCAGGGA
  GCCCGACTTAGAGAAGAAGTACTCCAAGCAGGTGGATGACCGATTTGGTGCCTATGTGGCCTGCGCCTCG
  CTTGTTTTCCTCTTCATCTGCTTTGTCCAGATCACCATTGTGCCCCACTCCCTGTTCATGCTGAGTTTCT
  ACCTGTCGTGTTTCCTGCTGCTGGCCTTGGTGGTGTTTGTGTCTGTGATCTATGCCTGTGTGAAGCTTTT
  CCCCACTCCCCTGCAGACACTCTCCAGGAAGATAGTGCGATCCAAGAAGAACAGCACCCTGGTCGGGGTA
  TTCACCATCACCCTGGTGTTCCTCTCGGCTTTTGTCAACATGTTCATGTGCAACTCTAAGAACCTGGTGG
  GCTGCCTGGCAGAGGAGCACAACATCACGGTGAACCAGGTCAACGCATGTCATGTGATGGAGTCGGCCTT
  CAACTACAGCCTGGGCGACGAGCAGGGCTTCTGTGGCAGCCCCCAGCCCAACTGCAACTTCCCAGAGTAC
  TTCACCTACAGCGTGCTGCTCAGCCTGCTGGCCTGCTCCGTGTTCCTGCAGATCAGCTGCATTGGAAAGC
  TGGTGCTCATGCTGGCCATTGAGTTCATCTATGTGCTCATCGTGGAGGTGCCCGGCGTCACACTCTTCGA
  CAACGCTGACCTTCTGGTCACCGCCAATGCCATAGACTTCAGCAACAACGGGACCTCCCAGTGCCCTGAG
  CATGCAACCAAGGTGGCGCTGAAGGTGGTGACGCCCATCATCATCTCTGTCTTCGTGCTGGCTCTGTATC
  TGCATGCCCAGCAGGTGGAGTCCACTGCTCGCCTTGACTTCCTCTGGAAACTGCAGGCCACAGAAGAGAA
  GGAGGAGATGGAGGAGCTGCAGGCCTACAATCGGCGCCTGCTGCACAACATTCTGCCCAAGGACGTGGCT
  GCCCACTTCCTGGCCCGGGAACGGCGCAATGACGAGCTGTACTACCAATCGTGCGAGTGTGTGGCTGTCA
  TGTTTGCCTCCATTGCCAACTTCTCCGAATTCTATGTAGAGCTAGAGGCCAACAATGAGGGCGTCGAGTG
  CCTACGGCTGCTCAATGAGATCATCGCAGACTTCGATGAGATCATCAGTGAGGATCGGTTCAGGCAGCTG
  GAAAAGATCAAGACCATAGGCAGCACCTACATGGCTGCCTCTGGCCTCAACGACTCCACCTATGACAAGG
  CAGGCAAGACCCACATCAAGGCTATTGCAGACTTCGCTATGAAGCTGATGGACCAAATGAAGTACATCAA
  TGAGCACTCCTTCAACAACTTCCAGATGAAGATTGGGCTCAACATTGGACCTGTAGTGGCTGGAGTCATT
  GGGGCTCGCAAGCCTCAGTATGACATCTGGGGCAATACAGTGAATGTGGCCAGCCGTATGGACAGCACTG
  GGGTGCCTGACCGCATCCAGGTTACTACAGATATGTACCAGGTGCTGGCCGCCAACACATACCAGCTGGA
  GTGCCGGGGTGTGGTCAAGGTCAAGGGCAAGGGTGAGATGATGACTTACTTCCTCAATGGAGGGCCTCCC
  CTCAGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225278 representing NM_001012765
  Red=Cloning site Green=Tags(s)
  
  MSGSKSVSPPGYAAQTAASPAPRGGPEHRAAWGEADSRANGYPHAPGGSTRGSTKRSGGAVTPQQQQRLA
  SRWRGGDDDEDPPLSGDDPLAGGFGFSFRSKSAWQERGGDDGGRGSRRQRRGAAGGGSTRAPPAGGSGSS
  AAAAAAAGGTEVRPRSVELGLEERRGKGRAAEELEPGTGIVEDGDGSEDGGSSVASGSGTGAVLSLGACC
  LALLQIFRSKKFPSDKLERLYQRYFFRLNQSSLTMLMAVLVLVCLVMLAFHAARPPLQIAYLAVLAAAVG
  VILIMAVLCNRAAFHQDHMGLACYALIAVVLAVQVVGLLLPQPRSASEGIWWTVFFIYTIYTLLPVRMRA
  AVLSGVLLSALHLAISLHTNSQDQFLLKQLVSNVLIFSCTNIVGVCTHYPAEVSQRQAFQETRECIQARL
  HSQRENQQQERLLLSVLPRHVAMEMKADINAKQEDMMFHKIYIQKHDNVSILFADIEGFTSLASQCTAQE
  LVMTLNELFARFDKLAAENHCLRIKILGDCYYCVSGLPEARADHAHCCVEMGMDMIEAISLVREVTGVNV
  NMRVGIHSGRVHCGVLGLRKWQFDVWSNDVTLANHMEAGGKAGRIHITKATLNYLNGDYEVEPGCGGDRN
  AYLKEHSIETFLILSCTQKRKEEKAMIAKMNRQRTNSIGHNPPHWGAERPFYNHLGGNQVSKEMKRMGFE
  DPKDKNAQESANPEDEVDEFLGRAIDARSIDRLRSEHVRKFLLTFREPDLEKKYSKQVDDRFGAYVACAS
  LVFLFICFVQITIVPHSLFMLSFYLSCFLLLALVVFVSVIYACVKLFPTPLQTLSRKIVRSKKNSTLVGV
  FTITLVFLSAFVNMFMCNSKNLVGCLAEEHNITVNQVNACHVMESAFNYSLGDEQGFCGSPQPNCNFPEY
  FTYSVLLSLLACSVFLQISCIGKLVLMLAIEFIYVLIVEVPGVTLFDNADLLVTANAIDFSNNGTSQCPE
  HATKVALKVVTPIIISVFVLALYLHAQQVESTARLDFLWKLQATEEKEEMEELQAYNRRLLHNILPKDVA
  AHFLARERRNDELYYQSCECVAVMFASIANFSEFYVELEANNEGVECLRLLNEIIADFDEIISEDRFRQL
  EKIKTIGSTYMAASGLNDSTYDKAGKTHIKAIADFAMKLMDQMKYINEHSFNNFQMKIGLNIGPVVAGVI
  GARKPQYDIWGNTVNVASRMDSTGVPDRIQVTTDMYQVLAANTYQLECRGVVKVKGKGEMMTYFLNGGPP
  LS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: RsrII-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012765
• ORF Size: 3786 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012765.5
• RefSeq Size: 5060 bp
• RefSeq ORF: 3789 bp
• Locus ID: 224129
• UniProt ID: P84309
• Cytogenetics: 16 B3
• Gene Summary: Catalyzes the formation of the signaling molecule cAMP in response to G-protein signaling. Mediates signaling downstream of ADRB1. Regulates the increase of free cytosolic Ca(2+) in response to increased blood glucose levels and contributes to the regulation of Ca(2+)-dependent insulin secretion.[UniProtKB/Swiss-Prot Function]