Orc4 (NM_001177313) Mouse Tagged ORF Clone
CAT#: MG224351
- TrueORF®
Orc4 (tGFP-tagged) - Mouse origin recognition complex subunit 4-like (S. cerevisiae) (Orc4l) transcript variant 2, (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001177313" in other vectors (3)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Orc4 |
Synonyms | mMmORC4; Orc4l; Orc4P |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG224351 representing NM_001177313
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCAGTCGTAAAACCAAGAGTAATGCACATGCAGAGTGTCTTTCACAGGTACAAAGAATTTTACGTG AAAGATTTTGTCATCATAGTCCACATAGCAACCTCTTTGGAGTGCAAGTACAATACAAGCATTTGATTGA GCTACTCAAAAGAACTGCTATCTATGGAGAAAGCAATTCTGTACTCATTGTTGGACCACGAGGATCAGGA AAGACCACATTATTAAATCATGCTTTGAAAGAACTCATGGAAATAGAAGTGAGTGAAAATGTGATACAAG TTCACTTAAATGGACTCCTGCAGACAAATGAAAAAATTGCTCTGAAAGAGATCACAAGACAATTAAATCT AGACAATGTAGTTGAAGATAAAGTTTTTGGTATGCTGGTGTGCCTTGAAGTCTCAAAGTACCGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG224351 representing NM_001177313
Red=Cloning site Green=Tags(s) MSSRKTKSNAHAECLSQVQRILRERFCHHSPHSNLFGVQVQYKHLIELLKRTAIYGESNSVLIVGPRGSG KTTLLNHALKELMEIEVSENVIQVHLNGLLQTNEKIALKEITRQLNLDNVVEDKVFGMLVCLEVSKYR TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001177313 |
ORF Size | 414 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001177313.1, NP_001170784.1 |
RefSeq Size | 1397 bp |
RefSeq ORF | 417 bp |
Locus ID | 26428 |
Cytogenetics | 2 C1.1 |
Gene Summary | Binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3 (By similarity). Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR224351 | Orc4 (Myc-DDK-tagged) - Mouse origin recognition complex, subunit 4 (Orc4), transcript variant 2 |
USD 165.00 |
|
MR224351L3 | Lenti ORF clone of Orc4 (Myc-DDK-tagged) - Mouse origin recognition complex, subunit 4 (Orc4), transcript variant 2 |
USD 465.00 |
|
MR224351L4 | Lenti ORF clone of Orc4 (mGFP-tagged) - Mouse origin recognition complex, subunit 4 (Orc4), transcript variant 2 |
USD 465.00 |
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