Abcb4 (NM_008830) Mouse Tagged ORF Clone

CAT#: MG223301

Abcb4 (tGFP-tagged) - Mouse ATP-binding cassette sub-family B (MDR/TAP) member 4 (Abcb4), (10ug).

Product Images

Circular map for MG223301

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Abcb4
• Synonyms: mdr-2; Mdr2; Pgy-2; Pgy2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223301 representing NM_008830, codon optimized.
  Due to the complexity of NM_008830, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCTCGAAGCTGCTCGCAACGGGACCGCTCGGAGGCTTGACGGAGATTTTGAGCTGGGGAGCATAA
  GCAACCAGGGCAGGGAAAAGAAAAAAAAGGTGAATTTGATTGGCCTGCTTACCCTTTTCCGCTACTCCGA
  TTGGCAGGACAAACTCTTCATGTTCCTGGGGACTCTGATGGCCATTGCTCATGGATCTGGATTGCCTCTG
  ATGATGATCGTCTTCGGGGAGATGACCGATAAGTTCGTCGATAATACAGGCAACTTCAGCTTGCCAGTCA
  ACTTTAGCCTCTCTATGCTGAACCCCGGACGAATCTTGGAGGAGGAGATGACAAGATATGCTTATTATTA
  TTCAGGACTGGGAGGCGGCGTGCTGGTTGCCGCCTACATACAGGTCAGTTTTTGGACCCTGGCAGCGGGG
  AGACAGATCAAGAAAATCCGACAGAAATTTTTTCATGCAATTCTCAGGCAGGAGATGGGCTGGTTTGATA
  TCAAAGGAACCACTGAGCTTAATACGCGGCTGACCGATGACGTGAGTAAAATCAGCGAGGGCATTGGAGA
  TAAAGTTGGTATGTTTTTCCAGGCCATCGCCACATTTTTTGCAGGATTCATCGTAGGATTTATTCGGGGA
  TGGAAGCTGACTTTGGTTATAATGGCTATCAGCCCTATCCTCGGACTCAGCACAGCTGTGTGGGCCAAGA
  TCCTGAGCACTTTTAGTGATAAAGAGCTCGCAGCATATGCCAAGGCTGGGGCGGTTGCTGAAGAGGCTCT
  GGGTGCCATTAGGACCGTCATCGCATTCGGAGGACAAAATAAAGAATTGGAGCGGTATCAGAAGCATCTC
  GAGAATGCTAAGAAAATTGGCATCAAGAAAGCAATTAGCGCAAATATATCCATGGGGATTGCTTTCCTGC
  TGATATATGCCTCCTACGCCCTGGCCTTTTGGTACGGGTCAACCCTGGTGATCTCTAAGGAGTACACCAT
  TGGCAATGCAATGACGGTTTTTTTTAGTATTCTGATCGGAGCCTTCTCCGTTGGACAGGCCGCCCCTTGC
  ATTGACGCTTTCGCTAACGCCCGGGGAGCAGCCTACGTTATATTTGATATTATTGATAACAATCCTAAAA
  TCGACTCCTTTAGCGAGCGGGGGCACAAGCCGGATAATATTAAAGGGAACCTGGAGTTCAGCGACGTTCA
  CTTCAGTTACCCAAGCCGCGCCAACATCAAAATTCTGAAAGGACTCAACCTCAAAGTTAAAAGCGGACAG
  ACAGTTGCCCTCGTGGGCAACAGTGGTTGCGGCAAGTCAACTACCGTCCAGCTTTTGCAGCGACTTTATG
  ATCCTACAGAAGGGAAAATCAGTATAGATGGGCAGGATATCAGGAATTTTAATGTTCGCTGCCTGAGGGA
  AATCATCGGAGTCGTTTCTCAGGAGCCGGTGCTGTTTTCCACTACCATAGCAGAAAACATTAGGTACGGC
  CGAGGGAATGTGACCATGGATGAGATAGAGAAGGCCGTCAAGGAAGCAAACGCTTACGATTTTATCATGA
  AACTGCCCCAGAAATTTGACACGCTGGTCGGTGACCGCGGGGCTCAGCTGAGTGGAGGCCAAAAGCAGAG
  GATAGCCATCGCTCGGGCCCTGGTGCGCAATCCAAAGATCTTGCTGCTGGACGAGGCCACATCAGCTCTT
  GACACAGAGTCCGAAGCGGAAGTGCAAGCCGCACTCGACAAGGCACGAGAAGGCAGAACTACTATTGTTA
  TCGCTCACAGACTTTCCACCATCAGGAATGCCGATGTTATCGCCGGATTCGAAGACGGGGTGATTGTAGA
  GCAGGGCTCTCACAGCGAGCTCATGAAGAAAGAGGGTATCTATTTTAGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223301 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDLEAARNGTARRLDGDFELGSISNQGREKKKKVNLIGLLTLFRYSDWQDKLFMFLGTLMAIAHGSGLPL
  MMIVFGEMTDKFVDNTGNFSLPVNFSLSMLNPGRILEEEMTRYAYYYSGLGGGVLVAAYIQVSFWTLAAG
  RQIKKIRQKFFHAILRQEMGWFDIKGTTELNTRLTDDVSKISEGIGDKVGMFFQAIATFFAGFIVGFIRG
  WKLTLVIMAISPILGLSTAVWAKILSTFSDKELAAYAKAGAVAEEALGAIRTVIAFGGQNKELERYQKHL
  ENAKKIGIKKAISANISMGIAFLLIYASYALAFWYGSTLVISKEYTIGNAMTVFFSILIGAFSVGQAAPC
  IDAFANARGAAYVIFDIIDNNPKIDSFSERGHKPDNIKGNLEFSDVHFSYPSRANIKILKGLNLKVKSGQ
  TVALVGNSGCGKSTTVQLLQRLYDPTEGKISIDGQDIRNFNVRCLREIIGVVSQEPVLFSTTIAENIRYG
  RGNVTMDEIEKAVKEANAYDFIMKLPQKFDTLVGDRGAQLSGGQKQRIAIARALVRNPKILLLDEATSAL
  DTESEAEVQAALDKAREGRTTIVIAHRLSTIRNADVIAGFEDGVIVEQGSHSELMKKEGIYFRLVNMQTA
  GSQILSEEFEVELSDEKAAGDVAPNGWKARIFRNSTKKSLKSPHQNRLDEETNELDANVPPVSFLKVLKL
  NKTEWPYFVVGTVCAIANGALQPAFSIILSEMIAIFGPGDDAVKQQKCNMFSLVFLGLGVLSFFTFFLQG
  FTFGKAGEILTTRLRSMAFKAMLRQDMSWFDDHKNSTGALSTRLATDAAQVQGATGTRLALIAQNTANLG
  TGIIISFIYGWQLTLLLLSVVPFIAVAGIVEMKMLAGNAKRDKKEMEAAGKIATEAIENIRTVVSLTQER
  KFESMYVEKLHGPYRNSVRKAHIYGITFSISQAFMYFSYAGCFRFGSYLIVNGHMRFKDVILVFSAIVLG
  AVALGHASSFAPDYAKAKLSAAYLFSLFERQPLIDSYSGEGLWPDKFEGSVTFNEVVFNYPTRANVPVLQ
  GLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPMAGSVLLDGQEAKKLNVQWLRAQLGIVSQEPILFD
  CSIAENIAYGDNSRVVPHDEIVRAAKEANIHPFIETLPQKYNTRVGDKGTQLSGGQKQRIAIARALIRQP
  RVLLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVIENGKVKEHGTHQQLLAQK
  GIYFSMVNIQAGTQNL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008830
• ORF Size: 3828 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008830.2, NP_032856.2
• RefSeq Size: 4083 bp
• RefSeq ORF: 3831 bp
• Locus ID: 18670
• UniProt ID: P21440
• Cytogenetics: 5 3.43 cM
• MW: 140.4 kDa
• Gene Summary: Energy-dependent phospholipid efflux translocator that acts as a positive regulator of biliary lipid secretion. Functions as a floppase that translocates specifically phosphatidylcholine (PC) from the inner to the outer leaflet of the canalicular membrane bilayer into the canaliculi between hepatocytes. Translocation of PC makes the biliary phospholipids available for extraction into the canaliculi lumen by bile salt mixed micelles and therefore protects the biliary tree from the detergent activity of bile salts (PubMed:8106172, PubMed:7912658, PubMed:7592705, PubMed:7814632, PubMed:8725158, PubMed:9366571). Plays a role in the recruitment of phosphatidylcholine (PC), phosphatidylethanolamine (PE) and sphingomyelin (SM) molecules to nonraft membranes and to further enrichment of SM and cholesterol in raft membranes in hepatocytes (By similarity). Required for proper phospholipid bile formation (PubMed:8106172). Indirectly involved in cholesterol efflux activity from hepatocytes into the canalicular lumen in the presence of bile salts in an ATP-dependent manner (PubMed:7814632, PubMed:8725158). May promote biliary phospholipid secretion as canaliculi-containing vesicles from the canalicular plasma membrane (PubMed:9366571). In cooperation with ATP8B1, functions to protect hepatocytes from the deleterious detergent activity of bile salts (PubMed:21820390). Does not confer multidrug resistance (PubMed:1990275).[UniProtKB/Swiss-Prot Function]