Tbk1 (NM_019786) Mouse Tagged ORF Clone

CAT#: MG223008

Tbk1 (tGFP-tagged) - Mouse TANK-binding kinase 1 (Tbk1), (10ug)

Product Images

Circular map for MG223008

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tbk1
• Synonyms: 1200008B05Rik; AI462036; AW048562
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223008 representing NM_019786
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGAGCACCTCCAACCATCTGTGGCTCCTGTCTGATATCCTAGGCCAGGGGGCCACTGCAAATGTCT
  TCCGAGGAAGGCATAAGAAAACTGGTGATCTCTATGCTGTCAAAGTATTTAATAACATAAGCTTCCTTCG
  CCCAGTGGATGTTCAAATGAGAGAATTTGAAGTGTTAAAAAAACTCAATCACAAAAACATTGTCAAGTTA
  TTTGCTATTGAAGAGGAGACAACAACAAGACATAAAGTGCTTATTATGGAGTTTTGTCCCTGTGGGAGTT
  TATACACTGTTCTAGAGGAGCCGTCCAATGCGTATGGACTTCCAGAATCAGAATTTCTCATTGTCTTACG
  AGATGTGGTGGGCGGGATGAATCATCTCCGAGAGAACGGCATAGTGCACCGAGATATCAAGCCAGGCAAC
  ATCATGCGCGTCATAGGGGAGGACGGCCAGTCTGTGTACAAACTCACGGATTTCGGCGCCGCTCGAGAGC
  TGGAGGACGATGAGCAGTTTGTGTCTCTGTACGGCACAGAAGAGTACCTGCATCCGGACATGTATGAAAG
  GGCAGTGCTAAGAAAGGACCATCAGAAGAAGTACGGGGCTACCGTTGATCTGTGGAGTGTTGGAGTGACA
  TTCTACCATGCAGCCACGGGGTCGCTGCCGTTTAGACCCTTCGAGGGGCCTCGGAGGAACAAAGAAGTAA
  TGTATAAAATAATCACTGGGAAGCCGTCTGGTGCAATATCTGGAGTACAGAAAGCAGAAAACGGACCAAT
  TGACTGGAGTGGAGACATGCCTCTCTCCTGTAGTCTTTCTCAGGGTCTTCAGGCACTGCTTACCCCAGTT
  CTTGCAAACATACTTGAAGCTGATCAGGAGAAGTGCTGGGGTTTTGACCAGTTCTTTGCAGAGACCAGTG
  ATGTGCTTCACCGAATGGTGATCCATGTCTTCTCGCTACAACACATGACGGCGCATAAGATTTACATTCA
  CAGCTATAACACTGCTGCTGTGTTCCATGAACTGGTCTATAAACAAACCAAGATTGTTTCCTCAAATCAA
  GAACTTATCTACGAAGGACGACGCTTAGTCCTAGAACTCGGACGACTAGCCCAGCATTTTCCTAAAACCA
  CAGAGGAAAATCCTATCTTTGTCACGAGCCGGGAACAACTCAATACCGTAGGACTGAGATATGAAAAAAT
  TTCCCTCCCTAAAATACATCCACGCTATGATCTGGATGGGGACGCCAGCATGGCCAAGGCAGTGACGGGG
  GTTGTGTGCTACGCCTGCAGAACTGCCAGTACCCTGCTGCTCTATCAAGAATTAATGCGAAAGGGGGTAC
  GGTGGCTGGTTGAACTGGTTAAGGATGATTACAACGAGACCGTCCACAAGAAGACGGAGGTAGTGATCAC
  ACTGGATTTCTGCATCAGGAACATTGAGAAGACTGTGAAAGTGTATGAGAAGTTGATGAAGGTCAACCTG
  GAAGCCGCAGAGCTGGGTGAGATTTCAGACATACACACCAAGCTGCTGAGACTTTCCAGTTCTCAGGGAA
  CAATAGAAAGCAGTCTTCAGGACATCAGCAGCAGGCTGTCTCCAGGGGGCTTGCTGGCCGACACCTGGGC
  ACATCAAGAAGGCACGCATCCAAGAGACAGGAATGTAGAAAAACTGCAGGTCCTGTTGAACTGCATCACA
  GAGATTTACTATCAGTTCAAAAAAGACAAAGCAGAACGCAGACTAGCTTATAATGAAGAACAGATCCACA
  AATTTGATAAGCAAAAATTGTATTACCATGCCACAAAAGCAATGAGCCACTTCTCAGAAGAATGTGTTAG
  AAAGTATGAAGCGTTTAAAGATAAGTCGGAAGAGTGGATGAGAAAGATGCTTCATCTTAGGAAGCAGCTG
  TTATCGCTAACTAATCAGTGTTTCGATATCGAAGAGGAAGTGTCCAAGTATCAAGACTATACTAACGAGT
  TACAAGAAACTCTGCCTCAGAAAATGCTCGCAGCCTCCGGCGGCGTCAAGCACGCCATGGCCCCGATCTA
  CCCCAGCTCTAACACCTTAGTGGAGATGACTCTTGGTATGAAGAAGTTAAAGGAGGAGATGGAAGGCGTG
  GTTAAGGAGCTGGCCGAGAACAATCATATTTTAGAAAGGTTTGGGTCTTTAACAATGGATGGTGGCCTTC
  GCAATGTGGACTGTCTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223008 representing NM_019786
  Red=Cloning site Green=Tags(s)
  
  MQSTSNHLWLLSDILGQGATANVFRGRHKKTGDLYAVKVFNNISFLRPVDVQMREFEVLKKLNHKNIVKL
  FAIEEETTTRHKVLIMEFCPCGSLYTVLEEPSNAYGLPESEFLIVLRDVVGGMNHLRENGIVHRDIKPGN
  IMRVIGEDGQSVYKLTDFGAARELEDDEQFVSLYGTEEYLHPDMYERAVLRKDHQKKYGATVDLWSVGVT
  FYHAATGSLPFRPFEGPRRNKEVMYKIITGKPSGAISGVQKAENGPIDWSGDMPLSCSLSQGLQALLTPV
  LANILEADQEKCWGFDQFFAETSDVLHRMVIHVFSLQHMTAHKIYIHSYNTAAVFHELVYKQTKIVSSNQ
  ELIYEGRRLVLELGRLAQHFPKTTEENPIFVTSREQLNTVGLRYEKISLPKIHPRYDLDGDASMAKAVTG
  VVCYACRTASTLLLYQELMRKGVRWLVELVKDDYNETVHKKTEVVITLDFCIRNIEKTVKVYEKLMKVNL
  EAAELGEISDIHTKLLRLSSSQGTIESSLQDISSRLSPGGLLADTWAHQEGTHPRDRNVEKLQVLLNCIT
  EIYYQFKKDKAERRLAYNEEQIHKFDKQKLYYHATKAMSHFSEECVRKYEAFKDKSEEWMRKMLHLRKQL
  LSLTNQCFDIEEEVSKYQDYTNELQETLPQKMLAASGGVKHAMAPIYPSSNTLVEMTLGMKKLKEEMEGV
  VKELAENNHILERFGSLTMDGGLRNVDCL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019786
• ORF Size: 2187 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019786.4, NP_062760.3
• RefSeq Size: 3031 bp
• RefSeq ORF: 2190 bp
• Locus ID: 56480
• UniProt ID: Q9WUN2
• Cytogenetics: 10 D2
• Gene Summary: Serine/threonine kinase that plays an essential role in regulating inflammatory responses to foreign agents (PubMed:10581243, PubMed:15210742, PubMed:15661922). Following activation of toll-like receptors by viral or bacterial components, associates with TRAF3 and TANK and phosphorylates interferon regulatory factors (IRFs) IRF3 and IRF7 as well as DDX3X (By similarity). This activity allows subsequent homodimerization and nuclear translocation of the IRFs leading to transcriptional activation of pro-inflammatory and antiviral genes including IFNA and IFNB (By similarity). In order to establish such an antiviral state, TBK1 form several different complexes whose composition depends on the type of cell and cellular stimuli (By similarity). Thus, several scaffolding molecules including FADD, TRADD, MAVS, AZI2, TANK or TBKBP1/SINTBAD can be recruited to the TBK1-containing-complexes (By similarity). Plays a key role in IRF3 activation: acts by first phosphorylating innate adapter proteins MAVS, TMEM173/STING and TICAM1 on their pLxIS motif, leading to recruitment of IRF3, thereby licensing IRF3 for phosphorylation by TBK1 (By similarity). Under particular conditions, functions as a NF-kappa-B effector by phosphorylating NF-kappa-B inhibitor alpha/NFKBIA, IKBKB or RELA to translocate NF-Kappa-B to the nucleus (By similarity). Restricts bacterial proliferation by phosphorylating the autophagy receptor OPTN/Optineurin on 'Ser-177', thus enhancing LC3 binding affinity and antibacterial autophagy (By similarity). Phosphorylates SMCR8 component of the C9orf72-SMCR8 complex, promoting autophagosome maturation (By similarity). Phosphorylates and activates AKT1 (By similarity). Seems to play a role in energy balance regulation by sustaining a state of chronic, low-grade inflammation in obesity, wich leads to a negative impact on insulin sensitivity (PubMed:23396211).[UniProtKB/Swiss-Prot Function]