Azin2 (NM_172875) Mouse Tagged ORF Clone

CAT#: MG222916

Adc (tGFP-tagged) - Mouse arginine decarboxylase (Adc), (10ug)

Product Images

Circular map for MG222916

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Azin2
• Synonyms: 4933429I20Rik; Ad; Adc; AZ; Azi2; B930082O19; Od; ODC-p; Odcp
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222916 representing NM_172875
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGCTATCTGAGTGAATCGGACTTTGTGATGGTGGAGGAGGGCTTCAGCACCCGGGATCTGCTGG
  AGGAGCTCACTCTGGGGGCCTCCCAGGCCACCTCGGGCAAGGTGGCTGCCTTCTTCGTGGCCGACCTGGG
  TGCTGTAGTGAGGAAGCACTTCTGCTTTCTGAAGCACCTGCCTCGAGTCCGGCCTTTTTATGCTGTCGGG
  TGCAACAGCAGCCTGGGCGTGCTGAAGGTTCTAGCCGAACTGGGGCTGGGCTTCAGCTGTGCCAACAAGG
  CAGAGATGGAGTTGGTCCAGCACATTGGTGTCCCTGCCAGTAAGATCATCTGTGCCAACCCCTGTAAGCA
  AGTTGCACAGATCAAGTATGCTGCCAAGCACGGGGTGAGGCTGCTGAGCTTCGACAATGAAGTGGAGCTG
  GCCAAGGTGGTCAAGAGCCACCCCAGTGCCAAGATGGTTCTGTGCATTGCTACCCAGGACTCCCACTCTC
  TGAATCACCTGAGCCTGAGGTTTGGGGCGTCGCTGAAATCCTGCAGACATCTGCTCGAGAACGCCAAGAA
  GAGCCACGTGGAGGTGGTGGGTGTGAGTTTTCACATTGGCAGTGGCTGTCCTGACCCTCAGGCCTATGCC
  CAGTCCATCGCGGATGCTAGGCTGGTGTTTCAGATGGGCGAGGAGCTGGGCCACACGATGAACATCCTGG
  ACCTTGGCGGCGGCTTTCCTGGCTTAGAGGGAGCCAAAGTGAGATTTGAAGAGATGGCCTCAGTAATTAA
  CTCAGCCTTGGACCTGTACTTCCCTGAGGGCTGCGGTGTGGACATCCTTGCTGAGCTGGGACGCTACTAC
  GTGACGTCTGCCTTCACTGTGGCTGTCAGCATCGTCGCCAAGAGGGAGGTTCTGGACCAGGCCAGCAGGG
  AAGAGCAAACCGGCGCAGCCCCTAAGAGCATCGTGTACTACCTTGATGAGGGCGTTTATGGGGTCTTCAA
  CTCAGTCCTGTTTGACAACACCTGCCCCACCCCCGCCCTGCAGAAGAAACCATCTGCGGATCAACCACTG
  TACAGCAGCAGCCTGTGGGGCCCAGCAGTTGAAGGCTGCGACTGTGTGGCTGAGGGCCTGTGGCTGCCGC
  AACTACAAGTAGGGGACTGGCTGGTCTTTGACAACATGGGTGCTTACACCGTGGACACAAAGTCCCTCCT
  TGGGGGGACCCAGGCCCGCAGAGTCACTTATGCCATGTCCCGGCTAGCCTGGGAAGCGCTTCGAGGGCAG
  CTGTTGCCTGCAGAAGAAGACCAGGACGCCGAGGGTGTGTGCAAACCTCTGTCCTGCGGCTGGGAGATCA
  CAGACACCTTGTGTGTGGGCCCTGTCTTCACCCCAGCAAGCATCATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222916 representing NM_172875
  Red=Cloning site Green=Tags(s)
  
  MAGYLSESDFVMVEEGFSTRDLLEELTLGASQATSGKVAAFFVADLGAVVRKHFCFLKHLPRVRPFYAVG
  CNSSLGVLKVLAELGLGFSCANKAEMELVQHIGVPASKIICANPCKQVAQIKYAAKHGVRLLSFDNEVEL
  AKVVKSHPSAKMVLCIATQDSHSLNHLSLRFGASLKSCRHLLENAKKSHVEVVGVSFHIGSGCPDPQAYA
  QSIADARLVFQMGEELGHTMNILDLGGGFPGLEGAKVRFEEMASVINSALDLYFPEGCGVDILAELGRYY
  VTSAFTVAVSIVAKREVLDQASREEQTGAAPKSIVYYLDEGVYGVFNSVLFDNTCPTPALQKKPSADQPL
  YSSSLWGPAVEGCDCVAEGLWLPQLQVGDWLVFDNMGAYTVDTKSLLGGTQARRVTYAMSRLAWEALRGQ
  LLPAEEDQDAEGVCKPLSCGWEITDTLCVGPVFTPASIM
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172875
• ORF Size: 1377 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172875.4, NP_766463.1
• RefSeq Size: 1917 bp
• RefSeq ORF: 1380 bp
• Locus ID: 242669
• UniProt ID: Q8BVM4
• Cytogenetics: 4 D2.2
• Gene Summary: The protein encoded by this gene belongs to the antizyme inhibitor family, which plays a role in cell growth and proliferation by maintaining polyamine homeostasis within the cell. Antizyme inhibitors are homologs of ornithine decarboxylase (ODC, the key enzyme in polyamine biosynthesis) that have lost the ability to decarboxylase ornithine; however, retain the ability to bind to antizymes. Antizymes negatively regulate intracellular polyamine levels by binding to ODC and targeting it for degradation, as well as by inhibiting polyamine uptake. Antizyme inhibitors function as positive regulators of polyamine levels by sequestering antizymes and neutralizing their effect. This gene encodes antizyme inhibitor 2, the second member of this gene family. Like antizyme inhibitor 1, antizyme inhibitor 2 interacts with all 3 antizymes and stimulates ODC activity and polyamine uptake. However, unlike antizyme inhibitor 1, which is ubiquitously expressed and localized in the nucleus and cytoplasm, antizyme inhibitor 2 is predominantly expressed in the brain and testis and localized in the endoplasmic reticulum-golgi intermediate compartment. Recent studies indicate that antizyme inhibitor 2 is also expressed in specific cell types in ovaries, adrenal glands and pancreas, and in mast cells. The exact function of this gene is not known, however, available data suggest its role in cell growth, spermiogenesis, vesicular trafficking and secretion. There has been confusion in literature and databases over the nomenclature of this gene, stemming from an earlier report that a human cDNA clone (identical to ODCp/AZIN2) had arginine decarboxylase (ADC) activity (PMID:14738999). Subsequent studies in human and mouse showed that antizyme inhibitor 2 was devoid of arginine decarboxylase activity (PMID:19956990). Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2014]