Dvl3 (NM_007889) Mouse Tagged ORF Clone

CAT#: MG222241

Dvl3 (tGFP-tagged) - Mouse dishevelled 3 dsh homolog (Drosophila) (Dvl3), (10ug)

Product Images

Circular map for MG222241

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dvl3
• Synonyms: b2b2866Clo
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222241 representing NM_007889
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGAGACCAAGATCATCTACCACCTGGACGGCCAGGAGACGCCGTACCTGGTGAAGCTGCCCCTGC
  CCGCCGAGCGCGTCACCTTGGCGGACTTTAAGGGCGTTCTGCAGCGGCCCAGCTATAAGTTCTTCTTCAA
  GTCTATGGACGACGATTTTGGGGTGGTGAAGGAAGAGATCTCGGACGACAATGCCAAGCTACCCTGCTTC
  AATGGCCGGGTGGTGTCCTGGCTGGTATCTGCCGAGGGCTCACACCCAGAGCCGGCCCCTTTCTGTGCTG
  ACAACCCGTCAGAGCTGCCACCTTCGATGGAACGCACAGGAGGCATTGGGGACTCTCGACCCCCCTCCTT
  CCACCCTCATGCTAGTGGGGGCAGCCAGGAGAATCTGGACAATGACACGGAGACCGACTCCTTGGTGTCT
  GCCCAACGAGAGCGGCCCCGACGGAGGGATGGCCCGGAGCACGCAGCCCGACTCAATGGAACCACAAAGG
  GTGAGCGGCGACGGGAGCCAGGCGGCTATGACAGTTCATCTACCCTCATGAGCAGCGAGCTGGAGACTAC
  CAGTTTCTTTGATTCCGATGAGGATGATTCCACCAGCAGGTTCAGCAGCTCCACAGAACAGAGCAGTGCC
  TCACGCCTCATGAGAAGACACAAGCGGCGGCGGCGGAAGCAGAAGGTCTCCCGGATTGAGCGGTCCTCGT
  CTTTCAGCAGCATCACAGACTCCACTATGTCTCTCAACATCATCACCGTCACTCTCAACATGGAAAAGTA
  TAACTTCTTGGGCATCTCCATTGTGGGCCAAAGCAATGAGCGAGGTGATGGGGGCATCTACATTGGCTCC
  ATCATGAAGGGTGGGGCTGTGGCAGCTGATGGACGCATTGAGCCAGGAGATATGCTACTGCAGGTAAATG
  AGATCAACTTCGAGAACATGAGCAACGATGACGCAGTCCGAGTCCTTCGGGAGATTGTGCACAAGCCAGG
  GCCCATCACTCTGACTGTAGCCAAGTGCTGGGACCCAAGTCCTCGCGGCTGCTTCACATTACCCCGGAGC
  GAGCCCATCCGTCCCATTGACCCTGCAGCCTGGGTCTCTCACACGGCAGCCATGACAGGCACCTTCCCCG
  CCTATGGCATGAGCCCCTCCTTAAGCACCATCACCTCTACCAGCTCCTCCATCACCAGCTCCATCCCTGA
  TACAGAGCGCCTAGATGATTTCCACCTCTCCATCCACAGTGACATGGCGGCCATCGTAAAAGCCATGGCC
  TCCCCTGAGTCAGGGTTGGAGGTCCGAGACCGCATGTGGCTCAAGATTACCATTCCCAATGCTTTCATCG
  GCTCGGATGTGGTGGACTGGCTGTACCACAACGTGGAAGGGTTCACCGACCGTCGGGAGGCCCGCAAGTA
  TGCTAGCAACCTGCTGAAAGCTGGTTTCATCCGCCATACCGTCAACAAGATCACGTTCTCCGAGCAGTGC
  TACTACATCTTTGGCGACCTCTGTGGTAACATGGCCAACCTATCGCTTCACGACCATGATGGCTCCAGTG
  GCGCCTCTGACCAGGACACACTGGCTCCTTTGCCGCACCCAGGAGCTGCCCCTTGGCCTATGGCTTTCCC
  TTACCAGTACCCACCACCTCCGCATCCCTACAACCCACACCCAGGCTTCCCAGAACTGGGCTACAGCTAC
  GGTGGGGGCAGCGCCAGCAGTCAGCACAGTGAAGGCAGTCGGAGCAGTGGCTCCAACCGAAGTGGCAGCG
  ACCGGCGCAAGGAGAAGGACCCAAAAGCTGGGGACTCCAAGTCAGGTGGTAGCGGCAGCGAGTCAGACCA
  CACTACACGCAGCAGTCTGAGGGGGCCACGGGAGCGGGCACCAAGCGAGCGATCAGGCCCAGCGGCCAGC
  GAGCACAGCCACCGCAGTCACCATTCGCTGACCAGCAGCCTGCGCAGCCACCACACACACCCAAGCTATG
  GCCCACCCGGAGTGCCCCCTCTCTATGGTCCCCCGATGCTGATGATGACCCCGCCGCCGGCAGCCATGGG
  TCCCCCAGGAGCCCCTCCAGGGCGTGACCTGGCCTCGGTGCCCCCGGAACTGACGGCCAGCAGACAGTCC
  TTCCGCATGGCAATGGGAAACCCCAGTGAGTTCTTTGTGGATGTGATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222241 representing NM_007889
  Red=Cloning site Green=Tags(s)
  
  MGETKIIYHLDGQETPYLVKLPLPAERVTLADFKGVLQRPSYKFFFKSMDDDFGVVKEEISDDNAKLPCF
  NGRVVSWLVSAEGSHPEPAPFCADNPSELPPSMERTGGIGDSRPPSFHPHASGGSQENLDNDTETDSLVS
  AQRERPRRRDGPEHAARLNGTTKGERRREPGGYDSSSTLMSSELETTSFFDSDEDDSTSRFSSSTEQSSA
  SRLMRRHKRRRRKQKVSRIERSSSFSSITDSTMSLNIITVTLNMEKYNFLGISIVGQSNERGDGGIYIGS
  IMKGGAVAADGRIEPGDMLLQVNEINFENMSNDDAVRVLREIVHKPGPITLTVAKCWDPSPRGCFTLPRS
  EPIRPIDPAAWVSHTAAMTGTFPAYGMSPSLSTITSTSSSITSSIPDTERLDDFHLSIHSDMAAIVKAMA
  SPESGLEVRDRMWLKITIPNAFIGSDVVDWLYHNVEGFTDRREARKYASNLLKAGFIRHTVNKITFSEQC
  YYIFGDLCGNMANLSLHDHDGSSGASDQDTLAPLPHPGAAPWPMAFPYQYPPPPHPYNPHPGFPELGYSY
  GGGSASSQHSEGSRSSGSNRSGSDRRKEKDPKAGDSKSGGSGSESDHTTRSSLRGPRERAPSERSGPAAS
  EHSHRSHHSLTSSLRSHHTHPSYGPPGVPPLYGPPMLMMTPPPAAMGPPGAPPGRDLASVPPELTASRQS
  FRMAMGNPSEFFVDVM
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007889
• ORF Size: 2148 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007889.3
• RefSeq Size: 2953 bp
• RefSeq ORF: 2151 bp
• Locus ID: 13544
• UniProt ID: Q61062
• Cytogenetics: 16 12.46 cM
• Gene Summary: Involved in the signal transduction pathway mediated by multiple Wnt genes.[UniProtKB/Swiss-Prot Function]