Sfswap (NM_172276) Mouse Tagged ORF Clone

CAT#: MG222227

Sfswap (tGFP-tagged) - Mouse serine/arginine-rich splicing factor 8 (Srsf8), (10ug)

Product Images

Circular map for MG222227

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Sfswap
• Synonyms: 1190005N23Rik; 6330437E22Rik; AI197402; AW212079; Sfrs8; Srsf8; SWAP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222227 representing NM_172276
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACGGTGCCGGCGGGGGCCGTGCCAAGCCGGAGAGGAAAGGCGGAGTGAAGGAGGAGGCCGGGCCGG
  GCGGCACCGGCACTGGGGGCAACCGGGTGGAGCTTCTGGTTTTCGGCTATGCCTGCAAGTTGTTCCGCGA
  CGACGAGCGGGCTTTGGCCCAGGAACAGGGACAGCACCTCATCCCCTGGATGGGGGACCCCAAGATCCTC
  ATCGACAGATACGACGGGCGTGGTCACCTGCATGACCTCTCTGCGTACGATGCTGAGTACGCCACGTGGA
  ACCGAGACTACCAGCTGTCTGAAGAGGAGGCTCGGGTAGAGGCGCTGTGTGATGAAGAGAGGTATTTAGC
  CTTGCATACGGACTTGCTTGAGGAGGAGGCAAGGCAAGAGGAAGAATACAAAAGATTGAGTGAAGCGCTG
  GCGGAGGATGGAAACTACAGTGCCGTAGGGTTCACTTACGGCAGCGACTATTATGACCCGTCAGAGCCCA
  CGGAAGAGGAGGAGCCTTCGAAGCAGAGAGAAAAAAATGAGGCTGAAAACTTGGAAGAAAATGAAGAACC
  TTTCATCGCCCCTTTAGGATTAAGCGTCCCGTCTGATGTGGAGCTGCCACCAACAGCCAAAATGCATGCC
  ATCATTGAGCGCACAGCCAACTTCGTATGCAAGCAGGGAGCACAGTTCGAGATCATGCTGAAGGCCAAGC
  AAGCTCGAAACTCACAGTTCGACTTCCTACGGTTTGATCACTACCTCAACCCCTACTATAAATTCATCCA
  GAAAGCCATGAAAGAAGGACGGTACACGGTGCTAGCTGAGAGCAAGAACGAGGAGAAGAAAAAGTCTGGG
  CCAACCTCTGACAACGAAGAGGAAGATGATGAGGAAGATGGGAGTTACCTGCACCCGTCTCTCTTTGCTT
  CCAAGAAGAGCAGCCGCCTGGAAGAGCTGATGAAGCCCTTGAAGGTGGTGGACCCGGATCACCCTCTAGC
  AGCCCTTGTCCGGAAAGCACAAGCTGACAGCTCAGCTCCAGCCCCACCCACTGCAGATGGCACACCTGCT
  CAGCCCTCCCAGGTGGAGTACACGGCAGACTCAACTGTGGCAGCCATGTACTACAGCTACTACATGTTGC
  CAGATGGTACCTACTGCCTGGCACCACCCCCACCTGGGATCGATGTGGCCACTTACTACAGCACCCTTCC
  TGCTGGAGTGACTGTGTCCAGCTCACCTGGTGTGACTACCACTGTACCACCGCCTCCTGGGACCACACCT
  CCTCCACCCCCAACCACAGCTGAACCAAGCAGCGGGGTTACCTCCACAACCACCACCACAAGTGCGCTTG
  CTCCCGTGGCCATCATACCCCCGCCCCCCGACATCCAGCCTGTGATTGACAAGCTGGCAGAGTACGTCGC
  TAGAAACGGCCTTAAATTTGAGACCAGTGTTCGAGCCAAGAACGATCAAAGATTTGAATTTCTTCAGCCC
  TGGCACCAGTACAACGCCTACTACGAGTTTAAGAAGCAGTTCTTCCTGCAGAAAGAAGGAGGCGGTAGCA
  CACAGGCAGCATCAACAGCTGAAGAGGCTCCCACAGAAACCGCTGTTGAAGAGTCGGGTGAAGCTGGAGA
  GGACGGCGCTCCTGAGGGTATGGCAGAGACTGGGGGAAGAGGCAGCGGGAAGAAGGAGGCTGGGTCCAGC
  AAGAGCACCGTGGACGGGAAGCTGGTAAAAGCTTCTTTTGCTCCAATAAGCTTTGCCATCAAAGCCAAAG
  AAAATGACCTCCTTCCCTTGGAGAAAAACCGTGTCAAGCTGGACGATGACAGTGAAGAGGATGAAGAAAG
  CAGGGAGTGCCAGGAAAGCACAAGCAGCGTGGCCAACCCCAGCCCTGCTGCCGCCCCGCCCTCCGTGGCT
  GTAGAGGAGAAGAAACCACAGCTTACCCAGGAGGAGCTGGAAGCCAAGCAAGCAAAACAAAAGCTGGAGG
  ACCGCCTTGCTGCTGCTGCCCGGGAAAAGCTAGCCCAGGCGTCCAAGGAATCAAAGGAGAAGCAGCTTCA
  GGCTGAACGTAAAAGGAAAGCAGCGTTGTTCTTACAAACCTTAAAAAATCCTCTTCCAGAAGCAGAAGTT
  GGAAAACTTGAGGAGAGTACTTTCGGGGTTGAGGACACTGGTGTCATGCCCTGTCCTCTGCTGGTTGGTG
  GTAGAACTCTGCCCATATTGGAAGGGAAGCCTCCAGAAAGGCCTTCAAACAGATGCAGAGATCCACCAAG
  AGAAGAAGAAAGAGAGAAAAAGAAGAAGAAGCACAAAAAACGGTCTCGGACAAGGTCCCGCTCTCCCAAG
  TACCACTCGTCTTCCAAGCCCAGGTCTAGATCCCACTCAAAAGCAAAGCACTCCCTGCCCAGTGCCTACC
  GGACAGTGCGGCGGTCAAGGTCACGGTCTCGGTCCCCAAGAAGGAGAGCTCACTCTCCTGAGAGACGGCG
  GGAAGAAAGGAGTGTCCCTACAGCCTACCGGATGAGCGGCAGCCCTGGGGTCAGCAGGAAAAGGACCCGT
  TCCAGAAGTCCCCACGAGAAGAAGAAGAAACGGCGCTCACGGTCTCGCACCAAAGCCAAGGCCCGGTCGC
  AGTCAACATCCCCGAGTAAACAGGCCGCACAGCGTCCCTCGGCCCACTCAGCCCACTCGGCCAGCATCTC
  TCCTGTGGAGAGCCGAGGTTCCTCCCAGGAACGCTCCAGGGGGGTTTCTCAGGAAAAAGATGGCCAGATC
  TCCTCAGCAATCGTCTCCTCTGTGCAGAGCAAAATAACTCAGGATCTCATGGCCAAAGTAAGAGCAATGC
  TCGCAGCTTCAAAAAACCTGCAAACCAGTGCATCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222227 representing NM_172276
  Red=Cloning site Green=Tags(s)
  
  MYGAGGGRAKPERKGGVKEEAGPGGTGTGGNRVELLVFGYACKLFRDDERALAQEQGQHLIPWMGDPKIL
  IDRYDGRGHLHDLSAYDAEYATWNRDYQLSEEEARVEALCDEERYLALHTDLLEEEARQEEEYKRLSEAL
  AEDGNYSAVGFTYGSDYYDPSEPTEEEEPSKQREKNEAENLEENEEPFIAPLGLSVPSDVELPPTAKMHA
  IIERTANFVCKQGAQFEIMLKAKQARNSQFDFLRFDHYLNPYYKFIQKAMKEGRYTVLAESKNEEKKKSG
  PTSDNEEEDDEEDGSYLHPSLFASKKSSRLEELMKPLKVVDPDHPLAALVRKAQADSSAPAPPTADGTPA
  QPSQVEYTADSTVAAMYYSYYMLPDGTYCLAPPPPGIDVATYYSTLPAGVTVSSSPGVTTTVPPPPGTTP
  PPPPTTAEPSSGVTSTTTTTSALAPVAIIPPPPDIQPVIDKLAEYVARNGLKFETSVRAKNDQRFEFLQP
  WHQYNAYYEFKKQFFLQKEGGGSTQAASTAEEAPTETAVEESGEAGEDGAPEGMAETGGRGSGKKEAGSS
  KSTVDGKLVKASFAPISFAIKAKENDLLPLEKNRVKLDDDSEEDEESRECQESTSSVANPSPAAAPPSVA
  VEEKKPQLTQEELEAKQAKQKLEDRLAAAAREKLAQASKESKEKQLQAERKRKAALFLQTLKNPLPEAEV
  GKLEESTFGVEDTGVMPCPLLVGGRTLPILEGKPPERPSNRCRDPPREEEREKKKKKHKKRSRTRSRSPK
  YHSSSKPRSRSHSKAKHSLPSAYRTVRRSRSRSRSPRRRAHSPERRREERSVPTAYRMSGSPGVSRKRTR
  SRSPHEKKKKRRSRSRTKAKARSQSTSPSKQAAQRPSAHSAHSASISPVESRGSSQERSRGVSQEKDGQI
  SSAIVSSVQSKITQDLMAKVRAMLAASKNLQTSAS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172276
• ORF Size: 2835 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172276.3, NP_758480.2
• RefSeq Size: 3319 bp
• RefSeq ORF: 2838 bp
• Locus ID: 231769
• UniProt ID: Q3USH5
• Cytogenetics: 5 G1.3
• Gene Summary: Plays a role as an alternative splicing regulator. Regulates its own expression at the level of RNA processing. Also regulates the splicing of fibronectin and CD45 genes. May act, at least in part, by interaction with other R/S-containing splicing factors. Represses the splicing of MAPT/Tau exon 10 (By similarity).[UniProtKB/Swiss-Prot Function]