Oaz2 (NM_010952) Mouse Tagged ORF Clone

CAT#: MG221992

Oaz2 (tGFP-tagged) - Mouse ornithine decarboxylase antizyme 2 pseudogene (Oaz2-ps), (10ug)

Product Images

Circular map for MG221992

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Oaz2
• Synonyms: AZ; AZ-; AZ-2; AZ2; Oaz; Oaz2-ps; Sez1; Sez15
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG221992 representing NM_010952
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATAAACACCCAGGACAGTAGTATTTTGCCGTTGAGTAAGTGTCCCCAGCTCCAGTGCTGCAGGCACA
  TTGTTCCAGGGCCTCTGTGGTGCTCCTGATGCCCCTCACCCACTGTCGAAGATCCCCGGTGGGCGAGGGG
  GCGGCAGGGATCCTTCTCTCTCAGCTCTAATATATAAGGACGAGAAGCTCACTGTAACCCAGGACCTCCC
  TGTGAACGATGGAAAACCTCACATCGTCCACTTCCAGTATGAGGTCACCGAGGTGAAGGTCTCTTCCTGG
  GATGCAGTCCTGTCCAGCCAGAGCCTGTTTGTAGAAATCCCAGATGGATTATTAGCTGATGGGAGCAAAG
  AAGGATTATTAGCACTGCTAGAGTTTGCTGAGGAGAAGATGAAAGTGAACTACGTCTTCATCTGCTTCAG
  GAAGGGCCGGGAAGACAGAGCTCCACTCCTGAAGACGTTCAGCTTCTTGGGCTTTGAGATTGTGCGTCCA
  GGTCATCCCTGTGTCCCCTCTCGGCCAGATGTGATGTTTATGGTTTACCCCCTGGACCAGAACTTGTCTG
  ATGAGGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010952
• ORF Size: 189 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010952.3, NP_035082.1
• RefSeq Size: 1861 bp
• RefSeq ORF: 571 bp
• Locus ID: 18247
• Cytogenetics: 9 C
• Gene Summary: The protein encoded by this gene belongs to the ornithine decarboxylase antizyme family, which plays a role in cell growth and proliferation by regulating intracellular polyamine levels. Expression of antizymes requires +1 ribosomal frameshifting, which is enhanced by high levels of polyamines. Antizymes in turn bind to and inhibit ornithine decarboxylase (ODC), the key enzyme in polyamine biosynthesis; thus, completing the auto-regulatory circuit. This gene encodes antizyme 2, the second member of the antizyme family. Like antizyme 1, antizyme 2 has broad tissue distribution, inhibits ODC activity and polyamine uptake, and stimulates ODC degradation in vivo; however, it fails to promote ODC degradation in vitro. Antizyme 2 is expressed at lower levels than antizyme 1, but is evolutionary more conserved, suggesting it likely has an important biological role. Studies also show different subcellular localization of antizymes 1 and 2, indicating specific function for each antizyme in discrete compartments of the cell. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2014]