Per3 (NM_011067) Mouse Tagged ORF Clone

CAT#: MG221637

Per3 (tGFP-tagged) - Mouse period homolog 3 (Drosophila) (Per3), (10ug)

Product Images

Circular map for MG221637

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Per3
• Synonyms: 2810049O06Rik; mPer3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG221637 representing NM_011067
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCCCTGTGGAGACCCGGCAGTACTTGGTGGCGACTGTCCCCAGACTAGGGGACCGGGGCTCCAGG
  GGGCGTCTGGCCAGGAGGGTCCTCTGCAGGGCACTTGCGTGGACAGCAGCCACAGTGAACACGAAGACCG
  AAACAGAATGTCTGAAGAGCTTATAATGGTTGTCCAAGAAATGAAAAAGTATTTCCCAGCCGAGAGGCAC
  ACTAAGCCCAGTACCCTAGATGCTCTTAACTATGCCCTGCGCTGTGTACACAGTGTGCAAGCAAACAGTG
  ACTTTTTCCAGAGTCTCGGTCCACGCGGAGCACACCAGGCAGATGTGACTGTATACAGTCTTGAGGACCT
  CACCGCTCTGGCTTCTGAACATACTTCTAAGAACACAGATACCTTCGCGGCCGTGTTTTCGTTTCTGTCT
  GGAAGGTTAGTGCACATTTCTGAGCAGGCTGCTTTGATCCTGAATTCTAAGAGGGGTTTCCTCAAGAGCG
  TGCACTTTGTCGACCTGCTTGCCCCTCAAGACGTGAGGGCGTTCTACGCGCACACTGCTCCAACTCAGCT
  TCCTTTCTGGAACAACTGGACCCAAAGAGCCTCGCAGTATGAATGTGCACCAGCGAAACCCTTTTTCTGC
  AGAATCTGTGGAGGTGGAGACAGAGAGAAGAGGCATTACTCCCCATTCCGGATCCTCCCCTATTTGGTTC
  ATGTACATAGCTCTGCCCAGCCAGAACCAGAGCCTTGCTGTCTAACACTGGTTGAAAAGATTCACTCTGG
  TTACGAAGCTCCTCGAATCCCTGTAGATAAAAGAATTTTTACCACAACACACACTCCAGGATGTGTGTTT
  CTTGAAGTAGATGAAAGAGCAGTGCCTTTGCTGGGTTACCTACCTCAGGATCTGATTGGAACATCGATCT
  TAACATACTTGCACCCAGAAGATCGGCCTCTGATGGTTGCCATACACCAAAAAGTTTTAAAGTATGCCGG
  CCACCCTCCGTTTGAACACTCGCCCGTCAGATTCTGCACTCAGAACGGAGAGTATGTCATTCTGGATTCC
  AGCTGGTCCAGCTTTGTCAACCCCTGGAGCCGGAAGGTCTCCTTCATCATTGGTCGACATAAAGTCCAAA
  CGAGTCCATTAAATGAAGATGTTTTTGCCACCAGAATAAAAAAGGCAGCCAGTAACGACAAAGACATAGC
  AGAATTACAAGAACAAATTCACAAACTTCTCTTGCAGCCGGTTCATGCTAGTGCTTCCAGTGGCTACGGG
  AGCCTGGGCAGCAGCGGCTCACAGGAGCAGCACGTCAGCATCACCTCTTCGAGTGAGTCCAGCGGGCACT
  GTCCGGAGGAAGGCCAGCATGAGCAGATGACCCTGCAGCAGGTCTATGCCAGTGTAAACAAAATTAAGAA
  TGTGGGCCAACAGCTCTACATCGAGTCCATGGCCAGATCATCAGTGAAGCCAGTGGCAGAGACGTGCGTG
  GAACCGCAGGGTGGTGATGAGCAGAAGGACTTATCTTCCTCTCAGACACTGAAAAATAAAAGCACCACGG
  ATACTGGCTCCGGTGGCAATCTGCAGCAAGAGCAGCCCAGCTCGTCCTATCAGCAGATGAACTGTATCGA
  CAGTGTCATCAGGTACCTGACAAGCTACAGCCTCCCGGCCTTGAAAAGAAAGTGCATCTCCTGCACAAAC
  ACATCTTCATCCTCAGAAGAAGCCAAGCCAATCCCGGAGGTGGACAGCAGCCAGAGAGACACGGAACAGC
  TCCTGGACATACGGAAACAGGGAACAACTGGACCATCCACAGACATCGAAGGAGGTGCTGCTCGGACCCT
  GTCCACCGCCGCACTGAGCGTGGCGTCTGGCATCAGCCAGTGCAGCTGCAGCAGCACCTCTGGCCACGCT
  CCGCCCCTACAGTCAGCAGAAAGTGTTGCCGTGGCGTGTAAGCCGTGGGCCCTGAGAACGAAGGCCTCTC
  ACCTGGCTGCAGGAGGATTTAAGCACGTGGGGCTCACAGCAGCTGTCCTCTCTGCACACACACAGAAGGA
  AGAGCAGAACTACGTTGACAGGTTCCGGGAAAAGATCCTGACCTCGCCCTACGGTTGCTATCTTCAGCAA
  GAGAGCAGAAACCGTGCTCAGTACTCCTGTGTTCAAGCAGGGTCCACTGCTAAGCACAGCAGATGTGCTG
  GAAGCGAGAGGCAGAAGCACAAACGAAAGAAGTTGCCAGCACCTGTGGACACCAGCAGCCCCGGTGCCCA
  CCTCTGTCCCCATGTCACAGGACTCCTCCCGGATGAGCAGCACTGGGGCCCATCCGCTAGCCCCTCCCCC
  CTCGGCGCAGGCTTAGCATTCCCCTCGGCCCTGGTAGTTCCCAGCCAGACCCCTTATCTCCTCCCCTCTT
  TTCCCCTCCAAGATATGGCCTCTCAGGGAGTGGGGGTCTCGGCAGCCTGGGGAGCTGCAGCCGGATGTCC
  ACCTCTGTCCGCCGGCCCCCAGGCTGTTGCCGCGTTCCCCTCCGCTTACGTGGATACTTTGATGACCATC
  TTCCTGCACAACGCCCCTCTCTTCCCTCTGTGGCCGCCCTCGTTCTCCCCATACCCATCCCTGGGGGCCG
  CAGGGTCTTCTGAACTGGCACCCTTAGTACCAGCAATGGCTCCAAACCCGGAACCAACCACTTCAGGCCA
  CAGCCAAAGGAGAGTGGAGGAGAACTGGGAGGCACACAGTGAAGAGCTTCCGTTCATTAGCTCACGGAGC
  AGTTCACCGTTACAGTTAAATTTACTCCAGGAAGAAATGCCTGCGCCGTCAGAGTCCGCAGACGCAGTGA
  GAAGAGGCGCTGGGCCAGACGCTAAGCATCACTGTGTTACAGGTCCCAGTGGCAGTAGGAGCCGTCACTG
  CACCTCTGGTGAGCTGGCCACGGCAACAGCGCAGCAGGAGTGTCCGTCTGCTGCTGCCTCAGGAAGCAGT
  GCCAGCAGTATATACTTCAGTAGCACTGACTATGCTTCTGAAGTCTCTGAAAACAGACAGAGGCCACAGG
  ATAGACAGAGAGACGAAGCCCTTCCCGGGGCGGCTGAAGAGTCCATCTGGAGAATGATAGAGCGGACACC
  AGAGTGTGTTCTCATGACATACCAGGTGCCCGAGAGGGGTCGAGAGGAGGTGCTGAAGCAGGACCTGGAG
  AAGCTCCAGAGCATGGAACAGCAGCAGCCCCTGTTCTCTCCCGCGCAGAGGGAGGAGCTGGCCAAGGTGC
  GCTCCTGGATCCACAGCCACACAGCCCCTCAGGAGGGACACCTCCAGAGCTGTGTCGCCTGTGAAGACAG
  AGGTTCAGTGGGTGACACTGCAGAGGTCCTGGAACAGCACCCAGCAGAAGACACCAGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG221637 representing NM_011067
  Red=Cloning site Green=Tags(s)
  
  MDPCGDPAVLGGDCPQTRGPGLQGASGQEGPLQGTCVDSSHSEHEDRNRMSEELIMVVQEMKKYFPAERH
  TKPSTLDALNYALRCVHSVQANSDFFQSLGPRGAHQADVTVYSLEDLTALASEHTSKNTDTFAAVFSFLS
  GRLVHISEQAALILNSKRGFLKSVHFVDLLAPQDVRAFYAHTAPTQLPFWNNWTQRASQYECAPAKPFFC
  RICGGGDREKRHYSPFRILPYLVHVHSSAQPEPEPCCLTLVEKIHSGYEAPRIPVDKRIFTTTHTPGCVF
  LEVDERAVPLLGYLPQDLIGTSILTYLHPEDRPLMVAIHQKVLKYAGHPPFEHSPVRFCTQNGEYVILDS
  SWSSFVNPWSRKVSFIIGRHKVQTSPLNEDVFATRIKKAASNDKDIAELQEQIHKLLLQPVHASASSGYG
  SLGSSGSQEQHVSITSSSESSGHCPEEGQHEQMTLQQVYASVNKIKNVGQQLYIESMARSSVKPVAETCV
  EPQGGDEQKDLSSSQTLKNKSTTDTGSGGNLQQEQPSSSYQQMNCIDSVIRYLTSYSLPALKRKCISCTN
  TSSSSEEAKPIPEVDSSQRDTEQLLDIRKQGTTGPSTDIEGGAARTLSTAALSVASGISQCSCSSTSGHA
  PPLQSAESVAVACKPWALRTKASHLAAGGFKHVGLTAAVLSAHTQKEEQNYVDRFREKILTSPYGCYLQQ
  ESRNRAQYSCVQAGSTAKHSRCAGSERQKHKRKKLPAPVDTSSPGAHLCPHVTGLLPDEQHWGPSASPSP
  LGAGLAFPSALVVPSQTPYLLPSFPLQDMASQGVGVSAAWGAAAGCPPLSAGPQAVAAFPSAYVDTLMTI
  FLHNAPLFPLWPPSFSPYPSLGAAGSSELAPLVPAMAPNPEPTTSGHSQRRVEENWEAHSEELPFISSRS
  SSPLQLNLLQEEMPAPSESADAVRRGAGPDAKHHCVTGPSGSRSRHCTSGELATATAQQECPSAAASGSS
  ASSIYFSSTDYASEVSENRQRPQDRQRDEALPGAAEESIWRMIERTPECVLMTYQVPERGREEVLKQDLE
  KLQSMEQQQPLFSPAQREELAKVRSWIHSHTAPQEGHLQSCVACEDRGSVGDTAEVLEQHPAEDTS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011067
• ORF Size: 3339 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011067.2
• RefSeq Size: 5953 bp
• RefSeq ORF: 3342 bp
• Locus ID: 18628
• UniProt ID: O70361
• Cytogenetics: 4 E2
• Gene Summary: This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. This gene is upregulated by Clock/Arntl heterodimers but then represses this upregulation in a feedback loop using Per/Cry heterodimers to interact with Clock/Arntl. Polymorphisms in this gene have been linked to sleep disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2014]