Ralgds (NM_001145836) Mouse Tagged ORF Clone

CAT#: MG221064

Ralgds (tGFP-tagged) - Mouse ral guanine nucleotide dissociation stimulator (Ralgds) transcript variant 2, (10ug)

Product Images

Circular map for MG221064

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ralgds
• Synonyms: Gnds; mKIAA1308; Rgds
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG221064 representing NM_001145836
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCAGCGCATGTGGGCTGAGGCGGCCGGGCCCATAGGGGGCACCGAGCCGTTGTTTCCCGGTTCCC
  GGCGCAGCCGCAGCGTGTGGGACGCCGTGCGCCTGGAGGTGGGCGTCCCCGACAGCTGCCCAGTGGTGCT
  GCACAGCTTCACGCAGCTCGACCCTGATCTGCCGCGCCTGGAGAGCTCCACGCAGGAGATTGGGGAGGAG
  CTGATCAACGGGGTCATCTACTCCATCTCCCTGCGCAAGGTCCAGCTACACCAAGGAGCCACTAAGGGCC
  AGCGCTGGCTAGGGTGTGAGAACGAGTCGGCTCTGAACCTCTATGAGACCTGCAAGGTGCGCACGGTGAA
  GGCTGGTACTCTGGAGAAGCTGGTGGAACACCTGGTGCCTGCCTTCCAGGGCAGTGACCTTTCCTACGTC
  ACTGTCTTCCTGTGCACCTACAGAGCCTTCACTACCACCCAGCAGGTGCTAGACCTGCTGTTCAAAAGAT
  ATGGCTGCATCCTCCCCTACTCCAGTGAGGACGGCGGACCGCAGGACCAACTCAAAAATGCCATCTCCTC
  CATCCTGGGCACCTGGCTGGACCAATACTCAGAGGATTTCTGTCAACCTCCGGACTTTCCCTGCCTCAAG
  CAGCTGGTGGCTTATGTACAGCTCAACATGCCTGGCTCAGATCTGGAGCGCCGCGCTCACCTTCTCCTGG
  CCCAGCTGGAGGACCTGGAGCCCAGTGAGGCTGAGTCTGAGGCCCTGTCCCCAGCTCCAGTGCTGTCTCT
  GAAGCCAGCTTCACAGCTAGAACCTGCACTGCTGCTGACGCCCAGCCAAGTGGTGACATCAACTCCAGTA
  CGAGAGCCCGCTGCGGCCCCAGTGCCAGTGCTGGCCTCCAGCCCAGTGGTGGCACCAGCTCCTGAGCTAG
  AACCAGTTCCAGAGCCACCTCAAGAGCCTGAGCCATCCCTAGCACTGGCTCCAGAGCTGGAGCCCGCCGT
  CTCACAGAGCCTGGAACTGGAGTCAGCTCCTGTGCCCACTCCTGCCTTAGAGCCTTCCTGGTCTCTGCCT
  GAAGCCACGGAGAATGGACTAACCGAGAAGCCTCACCTTCTGCTGTTCCCTCCTGACTTGGTGGCTGAAC
  AGTTTACTCTGATGGATGCAGAACTATTCAAGAAAGTCGTGCCCTACCACTGCCTGGGCTCCATCTGGTC
  CCAACGGGACAAGAAGGGCAAGGAGCACCTCGCGCCTACCATCCGCGCCACTGTCGCCCAGTTCAACAAC
  GTGGCCAACTGTGTCATTACTACCTGCCTTGGGGACCAGAGTATGAAGGCTCCGGACAGGGCCCGGGTGG
  TGGAACACTGGATCGAGGTGGCCAGGGAGTGCAGAGCGCTCAAGAATTTCTCCTCCCTCTACGCCATCCT
  CTCTGCTCTACAGAGCAATGCCATCCACCGCCTAAAGAAGACGTGGGAAGAGGTCTCCAGGGACAGCTTT
  CGAGTGTTCCAGAAACTGTCGGAGATCTTCTCTGATGAGAACAACTACTCCCTGAGCAGAGAGCTGCTCA
  TCAAGGAAGGAACCTCCAAGTTTGCCACACTGGAGATGAACCCTAGGAGAGCCCAGAGGCGGCAGAAGGA
  GACAGGAGTCATCCAGGGCACCGTTCCCTACCTGGGCACATTCCTCACTGACCTGGTGATGCTGGACACT
  GCCATGAAGGACTATCTCTATGGGAGACTGATCAACTTTGAAAAGAGAAGGAAGGAGTTCGAAGTCATTG
  CCCAGATCAAGTTGCTACAGTCAGCCTGCAACAACTACAGCATTGCTCCGGAAGAACACTTTGGAACATG
  GTTCCGAGCTATGGAGCGACTCAGTGAGGCTGAGAGCTACACCCTGTCGTGTGAGCTGGAGCCCCCGTCT
  GAGTCGGCCAGCAACACCCTGAGGAGCAAGAAAAGCACAGCCATTGTCAAGCGCTGGAGCGACCGCCAGG
  CTCCCAGCACGGAGCTCAGCACCAGTAGCAGTGCCCACTCCAAGTCCTGTGACCAGCTTCGGTGCAGCCC
  TTACCTCGGCAGCGGGGACATCACCGACGCGCTCAGTGTGCACTCAGCTGGCTCTTCCAGCTCTGATGTG
  GAGGAGATCAACATGAGCTTCGTCCCAGAGTCTCCTGATGGCCAGGAAAAGAAGTTCTGGGAGTCAGCCT
  CCCAGTCGTCCCCAGAGACCTCTGGCATCAGCTCCGCCTCCAGCAGCACCTCCTCTTCGTCAGCCTCCAC
  CACGCCCGTGTCTACCACGCGCACCCACAAGCGCTCCGTCTCAGGGGTCTGCAGCTACAGCTCCTCACTG
  CCTCTCTACAACCAGCAGGTGGGCGACTGCTGCATCATCAGGGTCAGCCTGGATGTGGACAACGGCAACA
  TGTACAAGAGCATCCTGGTGACCAGCCAGGATAAGGCTCCGACTGTCATCCGAAAAGCCATGGACAAACA
  CAACCTAGATGAGGACGAGCCGGAGGATTATGAGCTGGTGCAGATCATCTCAGAGGATCACAAGCTGAAG
  ATTCCAGAAAACGCCAATGTGTTCTATGCCATGAACTCTACCGCCAACTATGACTTCATCCTAAAGAAGC
  GGACCTTCACTAAGGGGGCTAAAGTCAAGCATGGAGCCAGCTCCACCCTCCCTCGTATGAAGCAGAAGGG
  ACTCAGGATTGCCAAAGGCATCTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG221064 representing NM_001145836
  Red=Cloning site Green=Tags(s)
  
  MVQRMWAEAAGPIGGTEPLFPGSRRSRSVWDAVRLEVGVPDSCPVVLHSFTQLDPDLPRLESSTQEIGEE
  LINGVIYSISLRKVQLHQGATKGQRWLGCENESALNLYETCKVRTVKAGTLEKLVEHLVPAFQGSDLSYV
  TVFLCTYRAFTTTQQVLDLLFKRYGCILPYSSEDGGPQDQLKNAISSILGTWLDQYSEDFCQPPDFPCLK
  QLVAYVQLNMPGSDLERRAHLLLAQLEDLEPSEAESEALSPAPVLSLKPASQLEPALLLTPSQVVTSTPV
  REPAAAPVPVLASSPVVAPAPELEPVPEPPQEPEPSLALAPELEPAVSQSLELESAPVPTPALEPSWSLP
  EATENGLTEKPHLLLFPPDLVAEQFTLMDAELFKKVVPYHCLGSIWSQRDKKGKEHLAPTIRATVAQFNN
  VANCVITTCLGDQSMKAPDRARVVEHWIEVARECRALKNFSSLYAILSALQSNAIHRLKKTWEEVSRDSF
  RVFQKLSEIFSDENNYSLSRELLIKEGTSKFATLEMNPRRAQRRQKETGVIQGTVPYLGTFLTDLVMLDT
  AMKDYLYGRLINFEKRRKEFEVIAQIKLLQSACNNYSIAPEEHFGTWFRAMERLSEAESYTLSCELEPPS
  ESASNTLRSKKSTAIVKRWSDRQAPSTELSTSSSAHSKSCDQLRCSPYLGSGDITDALSVHSAGSSSSDV
  EEINMSFVPESPDGQEKKFWESASQSSPETSGISSASSSTSSSSASTTPVSTTRTHKRSVSGVCSYSSSL
  PLYNQQVGDCCIIRVSLDVDNGNMYKSILVTSQDKAPTVIRKAMDKHNLDEDEPEDYELVQIISEDHKLK
  IPENANVFYAMNSTANYDFILKKRTFTKGAKVKHGASSTLPRMKQKGLRIAKGIF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145836
• ORF Size: 2685 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145836.1, NP_001139308.1
• RefSeq Size: 3617 bp
• RefSeq ORF: 2688 bp
• Locus ID: 19730
• Cytogenetics: 2 A3
• Gene Summary: Stimulates the dissociation of GDP from the Ras-related RalA and RalB GTPases which allows GTP binding and activation of the GTPases. Interacts and acts as an effector molecule for R-Ras, H-Ras, K-Ras, and Rap.[UniProtKB/Swiss-Prot Function]