Ralgds (NM_001145835) Mouse Tagged ORF Clone

CAT#: MG220969

Ralgds (tGFP-tagged) - Mouse ral guanine nucleotide dissociation stimulator (Ralgds) transcript variant 1, (10ug)

Product Images

Circular map for MG220969

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ralgds
• Synonyms: Gnds; mKIAA1308; Rgds
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG220969 representing NM_001145835
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCAGCGCATGTGGGCTGAGGCGGCCGGGCCCATAGGGGGCACCGAGCCGTTGTTTCCCGGTTCCC
  GGCGCAGCCGCAGCGTGTGGGACGCCGTGCGCCTGGAGGTGGGCGTCCCCGACAGCTGCCCAGTGGTGCT
  GCACAGCTTCACGCAGCTCGACCCTGATCTGCCGCGCCTGGAGAGCTCCACGCAGGAGATTGGGGAGGAG
  CTGATCAACGGGGTCATCTACTCCATCTCCCTGCGCAAGGTCCAGCTACACCAAGGAGCCACTAAGGGCC
  AGCGCTGGCTAGGGTGTGAGAACGAGTCGGCTCTGAACCTCTATGAGACCTGCAAGGTGCGCACGGTGAA
  GGCTGGTACTCTGGAGAAGCTGGTGGAACACCTGGTGCCTGCCTTCCAGGGCAGTGACCTTTCCTACGTC
  ACTGTCTTCCTGTGCACCTACAGAGCCTTCACTACCACCCAGCAGGTGCTAGACCTGCTGTTCAAAAGGT
  ACGGTAGATGTGACGCCCTCACGGCCTCCTCTAGATATGGCTGCATCCTCCCCTACTCCAGTGAGGACGG
  CGGACCGCAGGACCAACTCAAAAATGCCATCTCCTCCATCCTGGGCACCTGGCTGGACCAATACTCAGAG
  GATTTCTGTCAACCTCCGGACTTTCCCTGCCTCAAGCAGCTGGTGGCTTATGTACAGCTCAACATGCCTG
  GCTCAGATCTGGAGCGCCGCGCTCACCTTCTCCTGGCCCAGCTGGAGGACCTGGAGCCCAGTGAGGCTGA
  GTCTGAGGCCCTGTCCCCAGCTCCAGTGCTGTCTCTGAAGCCAGCTTCACAGCTAGAACCTGCACTGCTG
  CTGACGCCCAGCCAAGTGGTGACATCAACTCCAGTACGAGAGCCCGCTGCGGCCCCAGTGCCAGTGCTGG
  CCTCCAGCCCAGTGGTGGCACCAGCTCCTGAGCTAGAACCAGTTCCAGAGCCACCTCAAGAGCCTGAGCC
  ATCCCTAGCACTGGCTCCAGAGCTGGAGCCCGCCGTCTCACAGAGCCTGGAACTGGAGTCAGCTCCTGTG
  CCCACTCCTGCCTTAGAGCCTTCCTGGTCTCTGCCTGAAGCCACGGAGAATGGACTAACCGAGAAGCCTC
  ACCTTCTGCTGTTCCCTCCTGACTTGGTGGCTGAACAGTTTACTCTGATGGATGCAGAACTATTCAAGAA
  AGTCGTGCCCTACCACTGCCTGGGCTCCATCTGGTCCCAACGGGACAAGAAGGGCAAGGAGCACCTCGCG
  CCTACCATCCGCGCCACTGTCGCCCAGTTCAACAACGTGGCCAACTGTGTCATTACTACCTGCCTTGGGG
  ACCAGAGTATGAAGGCTCCGGACAGGGCCCGGGTGGTGGAACACTGGATCGAGGTGGCCAGGGAGTGCAG
  AGCGCTCAAGAATTTCTCCTCCCTCTACGCCATCCTCTCTGCTCTACAGAGCAATGCCATCCACCGCCTA
  AAGAAGACGTGGGAAGAGGTCTCCAGGGACAGCTTTCGAGTGTTCCAGAAACTGTCGGAGATCTTCTCTG
  ATGAGAACAACTACTCCCTGAGCAGAGAGCTGCTCATCAAGGAAGGAACCTCCAAGTTTGCCACACTGGA
  GATGAACCCTAGGAGAGCCCAGAGGCGGCAGAAGGAGACAGGAGTCATCCAGGGCACCGTTCCCTACCTG
  GGCACATTCCTCACTGACCTGGTGATGCTGGACACTGCCATGAAGGACTATCTCTATGGGAGACTGATCA
  ACTTTGAAAAGAGAAGGAAGGAGTTCGAAGTCATTGCCCAGATCAAGTTGCTACAGTCAGCCTGCAACAA
  CTACAGCATTGCTCCGGAAGAACACTTTGGAACATGGTTCCGAGCTATGGAGCGACTCAGTGAGGCTGAG
  AGCTACACCCTGTCGTGTGAGCTGGAGCCCCCGTCTGAGTCGGCCAGCAACACCCTGAGGAGCAAGAAAA
  GCACAGCCATTGTCAAGCGCTGGAGCGACCGCCAGGCTCCCAGCACGGAGCTCAGCACCAGTAGCAGTGC
  CCACTCCAAGTCCTGTGACCAGCTTCGGTGCAGCCCTTACCTCGGCAGCGGGGACATCACCGACGCGCTC
  AGTGTGCACTCAGCTGGCTCTTCCAGCTCTGATGTGGAGGAGATCAACATGAGCTTCGTCCCAGAGTCTC
  CTGATGGCCAGGAAAAGAAGTTCTGGGAGTCAGCCTCCCAGTCGTCCCCAGAGACCTCTGGCATCAGCTC
  CGCCTCCAGCAGCACCTCCTCTTCGTCAGCCTCCACCACGCCCGTGTCTACCACGCGCACCCACAAGCGC
  TCCGTCTCAGGGGTCTGCAGCTACAGCTCCTCACTGCCTCTCTACAACCAGCAGGTGGGCGACTGCTGCA
  TCATCAGGGTCAGCCTGGATGTGGACAACGGCAACATGTACAAGAGCATCCTGGTGACCAGCCAGGATAA
  GGCTCCGACTGTCATCCGAAAAGCCATGGACAAACACAACCTAGATGAGGACGAGCCGGAGGATTATGAG
  CTGGTGCAGATCATCTCAGAGGATCACAAGCTGAAGATTCCAGAAAACGCCAATGTGTTCTATGCCATGA
  ACTCTACCGCCAACTATGACTTCATCCTAAAGAAGCGGACCTTCACTAAGGGGGCTAAAGTCAAGCATGG
  AGCCAGCTCCACCCTCCCTCGTATGAAGCAGAAGGGACTCAGGATTGCCAAAGGCATCTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG220969 representing NM_001145835
  Red=Cloning site Green=Tags(s)
  
  MVQRMWAEAAGPIGGTEPLFPGSRRSRSVWDAVRLEVGVPDSCPVVLHSFTQLDPDLPRLESSTQEIGEE
  LINGVIYSISLRKVQLHQGATKGQRWLGCENESALNLYETCKVRTVKAGTLEKLVEHLVPAFQGSDLSYV
  TVFLCTYRAFTTTQQVLDLLFKRYGRCDALTASSRYGCILPYSSEDGGPQDQLKNAISSILGTWLDQYSE
  DFCQPPDFPCLKQLVAYVQLNMPGSDLERRAHLLLAQLEDLEPSEAESEALSPAPVLSLKPASQLEPALL
  LTPSQVVTSTPVREPAAAPVPVLASSPVVAPAPELEPVPEPPQEPEPSLALAPELEPAVSQSLELESAPV
  PTPALEPSWSLPEATENGLTEKPHLLLFPPDLVAEQFTLMDAELFKKVVPYHCLGSIWSQRDKKGKEHLA
  PTIRATVAQFNNVANCVITTCLGDQSMKAPDRARVVEHWIEVARECRALKNFSSLYAILSALQSNAIHRL
  KKTWEEVSRDSFRVFQKLSEIFSDENNYSLSRELLIKEGTSKFATLEMNPRRAQRRQKETGVIQGTVPYL
  GTFLTDLVMLDTAMKDYLYGRLINFEKRRKEFEVIAQIKLLQSACNNYSIAPEEHFGTWFRAMERLSEAE
  SYTLSCELEPPSESASNTLRSKKSTAIVKRWSDRQAPSTELSTSSSAHSKSCDQLRCSPYLGSGDITDAL
  SVHSAGSSSSDVEEINMSFVPESPDGQEKKFWESASQSSPETSGISSASSSTSSSSASTTPVSTTRTHKR
  SVSGVCSYSSSLPLYNQQVGDCCIIRVSLDVDNGNMYKSILVTSQDKAPTVIRKAMDKHNLDEDEPEDYE
  LVQIISEDHKLKIPENANVFYAMNSTANYDFILKKRTFTKGAKVKHGASSTLPRMKQKGLRIAKGIF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145835
• ORF Size: 2721 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145835.1, NP_001139307.1
• RefSeq Size: 3653 bp
• RefSeq ORF: 2724 bp
• Locus ID: 19730
• Cytogenetics: 2 A3
• Gene Summary: Stimulates the dissociation of GDP from the Ras-related RalA and RalB GTPases which allows GTP binding and activation of the GTPases. Interacts and acts as an effector molecule for R-Ras, H-Ras, K-Ras, and Rap.[UniProtKB/Swiss-Prot Function]