Hnrnpk (NM_025279) Mouse Tagged ORF Clone

CAT#: MG216549

Hnrnpk (tGFP-tagged) - Mouse heterogeneous nuclear ribonucleoprotein K (Hnrnpk), (10ug)

Product Images

Circular map for MG216549

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Hnrnpk
• Synonyms: hn; Hnrpk; KBB; KBBP; NOVA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG216549 representing NM_025279
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACCGAACAGCCAGAAGAAACCTTCCCCAACACCGAAACCAATGGTGAATTTGGTAAACGCCCTG
  CAGAAGATATGGAAGAGGAGCAAGCCTTTAAAAGATCTAGAAATACTGATGAGATGGTTGAATTGCGCAT
  TTTGCTTCAGAGCAAGAATGCTGGAGCAGTGATTGGAAAAGGAGGCAAGAATATTAAGGCTCTCCGTACA
  GACTACAATGCCAGTGTTTCAGTCCCAGACAGCAGTGGCCCCGAGCGCATACTGAGTATCAGTGCTGATA
  TTGAGACGATTGGAGAAATTCTGAAGAAAATCATCCCTACCTTGGAAGAGGGCCTGCAGTTGCCATCACC
  CACTGCAACCAGCCAGCTCCCGCTCGAATCTGATGCTGTGGAATGCTTAAATTACCAACATTATAAAGGA
  AGTGACTTTGATTGCGAGTTGAGACTGTTGATTCATCAGAGTCTGGCAGGAGGAATAATTGGTGTTAAAG
  GTGCTAAAATCAAAGAACTTCGAGAAAACACTCAGACAACAATCAAGCTTTTCCAGGAGTGCTGCCCTCA
  CTCTACTGACAGAGTTGTTCTTATTGGAGGAAAACCTGATAGGGTTGTAGAATGCATCAAGATCATCCTT
  GACCTTATATCTGAGTCTCCCATCAAAGGACGTGCACAACCTTATGATCCCAACTTTTATGATGAGACCT
  ATGATTATGGTGGTTTTACAATGATGTTTGATGACCGCCGAGGACGACCTGTGGGATTCCCCATGAGGGG
  AAGAGGTGGTTTTGACAGAATGCCTCCTGGTCGGGGTGGGCGTCCCATGCCTCCTTCTAGAAGAGATTAT
  GATGATATGAGCCCTCGTCGAGGACCTCCACCACCACCACCTGGTCGAGGTGGCCGGGGTGGCAGCAGAG
  CCCGGAATCTGCCTCTTCCTCCTCCACCACCACCCAGAGGGGGAGATCTAATGGCTTATGACAGAAGAGG
  AAGGCCTGGAGACCGCTATGATGGCATGGTTGGGTTCAGTGCTGATGAAACTTGGGATTCTGCAATTGAC
  ACATGGAGCCCATCAGAATGGCAAATGGCTTATGAACCACAGGGTGGTTCTGGATATGACTATTCTTATG
  CAGGGGGCCGTGGCTCATATGGTGATCTTGGCGGACCTATTATCACTACACAAGTAACTATTCCCAAAGA
  TTTGGCTGGATCTATTATTGGCAAAGGTGGTCAGCGGATTAAACAAATTCGTCATGAATCTGGAGCATCA
  ATCAAAATTGATGAACCTTTAGAAGGATCTGAAGATCGGATCATTACCATTACAGGAACACAGGACCAGA
  TACAGAACGCACAGTATTTGCTGCAGAACAGTGTGAAGCAGTATTCTGGAAAGTTTTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG216549 representing NM_025279
  Red=Cloning site Green=Tags(s)
  
  METEQPEETFPNTETNGEFGKRPAEDMEEEQAFKRSRNTDEMVELRILLQSKNAGAVIGKGGKNIKALRT
  DYNASVSVPDSSGPERILSISADIETIGEILKKIIPTLEEGLQLPSPTATSQLPLESDAVECLNYQHYKG
  SDFDCELRLLIHQSLAGGIIGVKGAKIKELRENTQTTIKLFQECCPHSTDRVVLIGGKPDRVVECIKIIL
  DLISESPIKGRAQPYDPNFYDETYDYGGFTMMFDDRRGRPVGFPMRGRGGFDRMPPGRGGRPMPPSRRDY
  DDMSPRRGPPPPPPGRGGRGGSRARNLPLPPPPPPRGGDLMAYDRRGRPGDRYDGMVGFSADETWDSAID
  TWSPSEWQMAYEPQGGSGYDYSYAGGRGSYGDLGGPIITTQVTIPKDLAGSIIGKGGQRIKQIRHESGAS
  IKIDEPLEGSEDRIITITGTQDQIQNAQYLLQNSVKQYSGKFF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025279
• ORF Size: 1389 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025279.3, NP_079555.1
• RefSeq Size: 1978 bp
• RefSeq ORF: 1392 bp
• Locus ID: 15387
• UniProt ID: P61979
• Cytogenetics: 13 B1
• Gene Summary: The protein encoded by this gene is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex, and is a poly-cytosine binding protein (PCBP). It is found in multiple subcellular compartments including the nucleus, cytoplasm and mitochondria. This gene product is thought to interact with RNA, DNA, and protein, and is involved in multiple cellular processes, including transcription, chromatin remodeling, DNA damage response, signal transduction, mRNA splicing, export, and translation. Multiple transcript variants and protein isoforms exist, with some isoforms containing a unique C-terminus. There are four pseudogenes of this gene, found on chromosomes 2, 3, 7 and 13. [provided by RefSeq, Aug 2014]