Parp8 (NM_001081009) Mouse Tagged ORF Clone

CAT#: MG216428

Parp8 (tGFP-tagged) - Mouse poly (ADP-ribose) polymerase family member 8 (Parp8), (10ug)

Product Images

Circular map for MG216428

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Parp8
• Synonyms: 2810430O08Rik; ARTD16; D13Ertd275e
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG216428 representing NM_001081009
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAAAGCCGACCTCCTCCTCCTCCTCCTCCTCCACCTCCTCCTCCTCTCTCACCCGGGATCACTTCCG
  AAACCACTTCGCCTTCAGCCCCTGCCTCGGCCAGAGGGATTTATTTAATGGGGATGTGTTCAAGGCAAGA
  GAGAATTCAGAAGGATATCGACGTCGTGATCCAGAAGTCAAGAGCCGAGAAAGACTGCCTGTTTGCAGAT
  TTCAGATACTCAGACTCCACCTTTACTTTCACCTACGTTGGCGGCCCCAAAAGTGTATCCTACTCCGTCC
  ACGTCTCAGAAGACTACCCAGACAACACGTATGTGTCAAGTTCAGAAAATGATGAGGATGTCCTAGTCAC
  TACAGACCCAATTCCTGTCATTTTCCATAGAATAGCAACAGAATTAAGGAAAACAAATGATATTAACTGT
  TGTTTGTCCATAAGATCTAAGTTACAAAAGGAAAATGGGGAGGAGTCAAGGCAAAATAGTACGGTGGAAG
  AAGACTCAGAGGGTGACAACGACTCTGAAGAATTTTATTATGGAGGACAGGTGAACTATGATGGGGAACT
  ACACAAGCACCCTCAGCTAGAAGCTGATCTGTCTGCGGTGAGAGAGCTCTATGGACCACATGCAGTTTCT
  CTCAGGGAATATGGGGCTATCGATGATGTAGATATTGATCTGCACATTGATGTCAGCTTTCTGGATGAGG
  AGATTGCTGTGGCCTGGGAAGTGATTAGAACAGAACCTATTATTGTCCGACTACACTGCTCTCTTACACA
  GTATTTAAACGGCCCAGTGCCCACGGTTGATGTCTTTCAGATTTCGACAAAAGAGCGGTTTGGATTGGGA
  CATCAACTGAAAAAAATCATGCAGACGTTTGTTTCTCAGCAGTGGAAACAGAGCAAAGACAAGTCCAATT
  GCCCGCACGGTAAGAAGCTGTCAGAGAAGAAGGTGAAGTCACCTCTGCATTTGTTTTCTACCCTGCGCAG
  GTCGCCCAGCTATCCTCCCCCTGGTTGTGGCAAAAGCAAATCCAAACTGAAACCCGAACAAGACGGCATC
  TCCAAAACACACAAGCTGCTGCGGAGGACTTGCTCCAGTACTGTCAAAGCCGATGATATGTGTGCCAAGT
  CGCACAGGACCTTCGGGCGCTCGCTGTCCAGCGACCCCAGGGCCGAGCAGGCGATGTCCACCATTAAATC
  GCACAAACTTTTGGGCCGGCCCTGCCCCTCAGCCGGCAAGCAAGAGGACTGCCTCACACTGAAGTCCCAT
  AAGTTATTGACTCGATCTTGTTCTGGAGACCCACGATGTGAGCACAACACCAACTTGAAGCCCCACAAAC
  TGCTAAGCAGATCTTACTCCAGTAATCTCAGAATGGAAGAGTTATATGGCCTGAAAAATCACAAATTGCT
  CAGCAAGTCTTACTCCAGTGCCCCCAAGACATCGAAAATGGAGCATTTCAAGGAACCCAACGCAGAGGGC
  AGGAGGCTCTCGCTTACCTCAGGGCTTATCGGTATTTTAACACCATCATCGTCTTCTTCCCAGCCTCCTA
  CAAATGGTGCAAAAAGCATTCCAATCCGAGACCGCGGCTTCCTGGTGCAGACCATTGAGTTTGCTGAGCA
  GCGGATCCCTGTACTGAATGAATATTGTGTGGTGTGTGATGAACCACACGTGTTTCAAAATGGCCCCATG
  CTTAGGCCCACAGTATGTGAGCGAGAGCTGTGTGTGTTTGCTTTTCAAACCCTGGGAGTGATGAATGAAG
  CTGCCGACGAGATCGCTACCGGAGCTCAGGTGGTAGATCTGCTTGTGTCCATGTGTAGATCTGCGTTGGA
  ATCTCCTAGAAAAGTTGTCATTTTTGAGCCATATCCTTCTGTGGTAGATCCTAATGACCCTCAGATGTTA
  GCCTTCAATCCCAGGAAGAAGAACTATGATCGTGTAATGAAAGCTCTGGACAGCATAACTTCCATCAGAG
  AAATGACACAGGCACCCTATCTGGAAATCAAGAAGCAGATGGATAAACAGGACCCCCTTGCTCATCCACT
  TCTGCAATGGGTCATCTCAAGTAATAGGTCACATATTGTGAAACTGCCAGTCAACAGGCAACTGAAGTTC
  ATGCACACGCCACACCAGTTCCTCCTCCTCAGCAGCCCACCAGCCAAAGAGTCCAACTTCAGAGCCGCCA
  AGAAGCTCTTCGGGAGCACCTTCGCATTTCATGGCTCTCACATTGAAAACTGGCACTCTATCCTGAGGAA
  TGGTCTGGTCGTTGCCTCTAACACGCGTCTGCAGCTCCACGGTGCAATGTACGGAAGTGGGATCTATCTT
  AGCCCGATGTCCAGCATATCATTTGGTTACTCAGGGATGAACAAGAAGCAGAAGGTGTCAAGCAAGGACG
  AGCCAGCTTCCAGTAGTAAAAGCAGCAATGCATCACAGTCTCAGAAAAAAGGACAGCAATCCCAGTTCCT
  GCAAAGCCGTAACTTGAAATGCATAGCCTTATGTGAAGTGATCACGTCACCTGACCTGCACAAACATGGC
  GAGATATGGGTTGTCCCGAACACGGATCATGTCTGTACAAGATTCTTTTTTGTTTATGAAGATGGCCAGG
  TGGGAGATGCCAATATCAACACACAAGAAGGAGGTATTCACAAAGAGATACTCCGTGTGATTGGTAATCA
  GACTGCGACTGGT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG216428 representing NM_001081009
  Red=Cloning site Green=Tags(s)
  
  MQSRPPPPPPPPPPPPLSPGITSETTSPSAPASARGIYLMGMCSRQERIQKDIDVVIQKSRAEKDCLFAD
  FRYSDSTFTFTYVGGPKSVSYSVHVSEDYPDNTYVSSSENDEDVLVTTDPIPVIFHRIATELRKTNDINC
  CLSIRSKLQKENGEESRQNSTVEEDSEGDNDSEEFYYGGQVNYDGELHKHPQLEADLSAVRELYGPHAVS
  LREYGAIDDVDIDLHIDVSFLDEEIAVAWEVIRTEPIIVRLHCSLTQYLNGPVPTVDVFQISTKERFGLG
  HQLKKIMQTFVSQQWKQSKDKSNCPHGKKLSEKKVKSPLHLFSTLRRSPSYPPPGCGKSKSKLKPEQDGI
  SKTHKLLRRTCSSTVKADDMCAKSHRTFGRSLSSDPRAEQAMSTIKSHKLLGRPCPSAGKQEDCLTLKSH
  KLLTRSCSGDPRCEHNTNLKPHKLLSRSYSSNLRMEELYGLKNHKLLSKSYSSAPKTSKMEHFKEPNAEG
  RRLSLTSGLIGILTPSSSSSQPPTNGAKSIPIRDRGFLVQTIEFAEQRIPVLNEYCVVCDEPHVFQNGPM
  LRPTVCERELCVFAFQTLGVMNEAADEIATGAQVVDLLVSMCRSALESPRKVVIFEPYPSVVDPNDPQML
  AFNPRKKNYDRVMKALDSITSIREMTQAPYLEIKKQMDKQDPLAHPLLQWVISSNRSHIVKLPVNRQLKF
  MHTPHQFLLLSSPPAKESNFRAAKKLFGSTFAFHGSHIENWHSILRNGLVVASNTRLQLHGAMYGSGIYL
  SPMSSISFGYSGMNKKQKVSSKDEPASSSKSSNASQSQKKGQQSQFLQSRNLKCIALCEVITSPDLHKHG
  EIWVVPNTDHVCTRFFFVYEDGQVGDANINTQEGGIHKEILRVIGNQTATG
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001081009
• ORF Size: 2673 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081009.1, NP_001074478.1
• RefSeq Size: 3088 bp
• RefSeq ORF: 2676 bp
• Locus ID: 52552
• UniProt ID: Q3UD82
• Cytogenetics: 13 65.32 cM
• Gene Summary: Mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins.[UniProtKB/Swiss-Prot Function]