Iqgap3 (NM_001033484) Mouse Tagged ORF Clone

CAT#: MG215325

Iqgap3 (tGFP-tagged) - Mouse IQ motif containing GTPase activating protein 3 (Iqgap3), (10ug)

Product Images

Circular map for MG215325

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Iqgap3
• Synonyms: AI593484; D030034H08
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG215325 representing NM_001033484
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCGTGCGCGGCAGGCCGAGCTCGGACAGCCTATGAGCGCCTCACAGCTGAGGAAATGGATGAGC
  AGAGGCGACAGAATGTCGCCTATCAGTACCTCTGCCGGCTGGAGGAAGCCAAGCGTTGGATGGAGGTGTG
  CTTGAAGGAGGAGCTTCCTTCCCCTGTGGAGCTGGAGGAGAGCCTCCGGAATGGAGTGCTACTGGCCAAG
  CTGGGCCACTGTTTTGCACCCTCTGTGGTCCCCCTGAAGAAGATCTACGACGTGGAGCAGCTACGCTACC
  AGGCGACTGGCTTGCATTTCCGGCACACGGACAACATCAACTTTTGGCTCTCTGCGGTAGCCCACATCGG
  TCTGCCTTCGATCTTCCTCCCAGAGACCACAGACATCTATGACAAAAAGAACATGCCTCGGGTGATCTAC
  TGCATTCACGCTCTCAGTCTCTTCCTCTTCCGGCTGGGATTGGCTCCACAGATCCATGACCTGTATGGGA
  AAGTGAAATTCACAGCTGAGGAACTCGGCAACATCGCCTCTGAACTGGCCAAATATGGCCTCCAGTTACC
  GGCCTTCAGCAAGATCGGGGGCATCCTGGCCAATGAGTTTTCAGCCGATGAGGCTGCAGTCCATGCAGCT
  ATTCTGGCCATCAATGATGCCGTGGAGCGAGGGGTGGTAGAGGACACTCTGGTCACCTTGCAGAATCCCA
  ATGCTCTCCTGGGGAATCTTCGAGAACCTCTGGCAGCTGTCTACCAAGAGCTGCTGGCCCTAGCCAAGAT
  GGAGAAGGCGGCCAATGCCAGGAATCATGACGATGGTCAGGAGCAGGACATCTATGAATCCTGCCTCACC
  CAGGCAGAGATCCAGGGCCACATCAACCTCGCCAACGTCCAGGGGGCACTGGAAGTTGTTGACGACGCTC
  TGGAGAGGCAGAACCCTGGGGCCTTGCTTGAGGCCCTTCACGATCCTGTCCTGGCCCTACAAGGGGTGAG
  AGGAACCTTTGCTGACTGGTACCTGGAACAGCTGACTTCAGACCGAGAGCAGAAATCACAGGAGCTGGGC
  CTGGTGAGACTTCTAGAAAAGGAGGAGATCCAGGCTGGTGTGGCTGTAGCCAATGAGAAGGGGGATGAAG
  AGCAAACTATGCTCCAGGCTGTGTGGAGGATCAACAAAGCCATCCGGAGGGGAGTGGCTGCTGACACCGT
  GAAGGAGCTCATGTGCCCTGAAGCCCAGCTGCCCCGAGTCTACCCCTTTGCCTCTGCTTTTTACCAGCAA
  GAGCTGGCTCTCCTCCAGAAGCAGCAACAGGGGGAGCTGGGCCAGGAGGAGCTCTTTGTGGCTGTGGAGA
  TGCTGTCAGCCGTGGTTCTGATTAACCGTGCTCTGGAAGCTGGAGATGTCTGTGCCTTTTGGGACAATCT
  AGTGAATCCCGCAACTGGCCTAGCCCAGGTAGAAGAAGAAAATGCCCAACGTTACTTTGATGCCCTGGTG
  AAGGTTCAGCAGTTTCAAGGAACACATAGAGGCATCCTGAGCTGGAATGACCTGCAGGCTGCTGTGAGCC
  AGGTCAATGAACAGGTCCAGGAAGAAACTGATCAGGTCCTCGCCATCAGCCTCATCAATGAGGCTCTGGA
  CCAGGGCTGTCCTGAAAAGACTCTGTCTGCCCTGCTGCTTCCTGCAGCTGGCCTGGAAGATGTCAGCCTT
  CATGTTGCACCTCGGTACCATCTCCTCCTTGTGGCTGCTAAGAGGCAGAAAGCACGGGTGACAGGAGACC
  CTGGAGCTGTGCTGTGGCTGGAGGAGATCCGCCAAGGAGTGGCCAGAGCCAACGAGGACACAAGCACGGC
  TCAGAGAATAGCCCTTGGTGTGGCTGCTATCAACCAGGCCATCAAGGAGGGCAAGGCAGCCCAGACAGAG
  CGTGTGTTGAGGAACCCCAATGTGGCCCTCCGAGGGATAGTTCCTGACTGCGCTAAAAGCTACCAGCAGG
  CCCTGGAAGGCGCAGCGGCAAAGAAGCAATGCCCTGGGGACACAGCCTTCTGGGTTCAACATGACATGAA
  GGATGGCACCGCCTACTACCTTCATCTGCAGACCTTTCAGGGGACTTGGGAGCATCCTTCCGGCTGCCAC
  CTCAATACCTCCCATCTAACCTGGGAGGAGATTCAGTCAGTCATCACCAAGGTCACTGCTGCCCATGACC
  GCCAACTGCTCTGGAAGGCCAATGTTGGCTTTGTCATCAAGCTCCAGGCCCGCCTTCGTGGCTTCCTTGT
  TCGCCAGAAGTTTGCTGAGTCTTCCCACGTCTTCAGGACCTTGCTTCCAGCCGTCATCAAGATCCAGGCT
  CACTGGCGGGGTTATAGACAACGGAAGGCTTACCAGGAGCGGCTGCAGCATTTTAAAGCGAACCTGGATG
  CTATAATCAAGATCCAGGCCTGGGCCCGAATGTGGGCAGCACGGAGGCGATACCTCAGGCGTCTGCGATA
  CTTCCAGAAGAACGTTGACTCCGTTGTGAAGATTCAAGCATTTTTCCGAGCCAGGAAAGCCCGTGATGAC
  TACAGGATGTTAGTGCACGCACGCCATCCTCCCCTCTGTGTAGTCCGAAAATTCGCTCACCTCTTGAACC
  AGAGTCAGGAGGACTTCTCAGCTGAATCGGAGTTGCTAAAGCTCCAAGAGGAAGTGGTTAGGAAGATCCG
  CTCTAACCAGCAGCTGGAGCAGGACCTCAACCTCATGGACATCAAGATTGGTCTGCTCGTCAAGAACCGC
  ATCACCCTGCAGGAGGTAGTCTCCCACTGCAAGAAGCTGACCAAGAAGAATAAGGAGCAACTGTCGGACA
  TGATGATCCTGGACAAACAGAAGGGATTAAAGTCACTGAGCAGAGAGAAACGGCAGAAGCTAGAAGCCTA
  CCAGCACCTCTTCTACCTGCTGCAGACTCAGCCCATCTATTTGGCCAAGCTGATCTTTCAGATGCCCCAG
  AACAAAACTACCAAGTTCATGGAAGGTGTGATTTTCAGTCTGTACAACTATGCCTCCAACCGTCGGGAGG
  CCTACCTTCTGCTCCAGCTGTTCAGGACGGCGCTCCAGGAGGAAATAAAGTCAAAGGTGGAACAGCCCCA
  GGATGTGGTGACAGGCAACCCCACAGTGGTGAGACTGGTGGTGAGGTTCTACCGCAATGGGCGGGGACAG
  AGCGCTCTGCAGGAGATCCTTGGCAAGGTCATCCAGGATGTGCTAGAAGACAGGTCAGTCAGCATCCACA
  CAGACCCTGTCCACATCTATAAGAGCTGGATCAACCAGGAGGAGGCCCAGACTGGACAGCGCAGCCATCT
  CCCCTATGACGTCACCCCTGAGCAGGCTCTGAGCCACTCTGAAGTGCAGAGACGATTAGACATCTCCCTG
  CGCAGTCTCCTCACCTTGACTGAGAAGTTCTTTGTCGCCATCAGTTCATCTGTGGACCACATTCCGTATG
  GGATGAGGTATATGGCCAAAGTCCTGAAAACAACCTTGGAGAAGAAGTTCCCCAATGCCACCGAGAGGGA
  CATCTATAAGGTGGTTGGGAACCTCCTGTACTACCGCTTCCTGAACCCAGCTGTGGTCGCTCCTGATGCC
  TTTGACATTGTGGCCATGGCGGCAGGCAGCACCCTGGCCGCCCCACAGCGCCATGCGCTGGGAGCCGTGG
  CTCAGCTCCTACAGCACGCTGCTGCTGGCCAGATCTTCTCTGGGGAGAGCCGGCACCTTCGAGTCCTGAA
  TGACTACCTGGAGGACTTGCATCTCAAGTTCAGGAAGTTCATCTGCAGAGCCTGCCGGGTACCAGAGCCA
  GAGGAGCGCTTCGCCATCGATGAATACTCAGACATGGTGGCTGTGGCCAAACCCATGGTGTACATCACTG
  TAGGGGAGCTCATCGGCACACACAGGCTCTTGCTCGAACACCAAGACCAGCTGGCCCCAGGTCACCAAGA
  CCCACTGCACCAGCTCCTAGAAGACCTTGGGGAGCCACCCACCATCTCTGACCTCATTGGGGACAACATA
  GCCACAGACGGGCATGTGGACCTGAGCAGGCTCGAAGTGTCCCTGACACTCACCAACAAGTTTGAAGGGC
  TGGAGACAGATGCTGACCACAACAGCAACCAAAGTCTGCTTCTGAGCACCAAACAGATGCTGGCCGACCT
  CATCCAGTTCCACCCTGGGGAGTCCCTGGAAGAGATCCTGACCTCCTCGGCTCCCAGAGAGCATGAAGAA
  GCCCATCGTCGGTTGATGTGTTGGCGCCAAGCCTGTGACACCCAGAAACCAGAGCCACTTCGACGACACC
  ACTCACTAATGGCACACAGTCTCCTGTCACTGTCGGAGAAGCAGCAACGCGTTCTGCGGAACCTGCGTCG
  GCTACAGGGCCTGGGGCTGGTCAGGGCCAGTGACTGCTACCAGGGACTTGTAGACGAGCTGGCCAAGGAC
  ATCTGCAACCAGCGTAGGCACCGGCAACGGCGGAAGGCAGAGATGCTGAGGCTCCGGACCACACTGCAGG
  GCCTAGATGCAAAAACCATCTTCTATGAGGAACAGGGTGACTACTACAACCAATACATCCAGGCCTGCCT
  TGACCACCTGGCCCCCAAACCCAAGAGTTCTGGGAAGGGGAAGAAGCAGCCGTCTCTTCACTACACAGCC
  GCCCAGCTCCTAGAAAAGGGCGTCTTGGTGGAAATTGAAGATCTCCCTGTTTCTCACTTCAGAAACGTCA
  TCTTTGACATCACTCCTGGAGACGAGGCAGGAAGGTTTTTCGTCAATGCCAAGTTCCTGGGTGTGGACAT
  GGAGAAGTTTCAGCTCCACTACCAGGACCTCTTGCAGCTGCAGTATGAGGGTGTGGCTGTCATGAAACTC
  TTTAACAAAGCCAAAGTCAACGTCAATCTCCTCATCTTCCTGCTCAACAAGAAGTTCCTTCGGAAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG215325 representing NM_001033484
  Red=Cloning site Green=Tags(s)
  
  MEPCAAGRARTAYERLTAEEMDEQRRQNVAYQYLCRLEEAKRWMEVCLKEELPSPVELEESLRNGVLLAK
  LGHCFAPSVVPLKKIYDVEQLRYQATGLHFRHTDNINFWLSAVAHIGLPSIFLPETTDIYDKKNMPRVIY
  CIHALSLFLFRLGLAPQIHDLYGKVKFTAEELGNIASELAKYGLQLPAFSKIGGILANEFSADEAAVHAA
  ILAINDAVERGVVEDTLVTLQNPNALLGNLREPLAAVYQELLALAKMEKAANARNHDDGQEQDIYESCLT
  QAEIQGHINLANVQGALEVVDDALERQNPGALLEALHDPVLALQGVRGTFADWYLEQLTSDREQKSQELG
  LVRLLEKEEIQAGVAVANEKGDEEQTMLQAVWRINKAIRRGVAADTVKELMCPEAQLPRVYPFASAFYQQ
  ELALLQKQQQGELGQEELFVAVEMLSAVVLINRALEAGDVCAFWDNLVNPATGLAQVEEENAQRYFDALV
  KVQQFQGTHRGILSWNDLQAAVSQVNEQVQEETDQVLAISLINEALDQGCPEKTLSALLLPAAGLEDVSL
  HVAPRYHLLLVAAKRQKARVTGDPGAVLWLEEIRQGVARANEDTSTAQRIALGVAAINQAIKEGKAAQTE
  RVLRNPNVALRGIVPDCAKSYQQALEGAAAKKQCPGDTAFWVQHDMKDGTAYYLHLQTFQGTWEHPSGCH
  LNTSHLTWEEIQSVITKVTAAHDRQLLWKANVGFVIKLQARLRGFLVRQKFAESSHVFRTLLPAVIKIQA
  HWRGYRQRKAYQERLQHFKANLDAIIKIQAWARMWAARRRYLRRLRYFQKNVDSVVKIQAFFRARKARDD
  YRMLVHARHPPLCVVRKFAHLLNQSQEDFSAESELLKLQEEVVRKIRSNQQLEQDLNLMDIKIGLLVKNR
  ITLQEVVSHCKKLTKKNKEQLSDMMILDKQKGLKSLSREKRQKLEAYQHLFYLLQTQPIYLAKLIFQMPQ
  NKTTKFMEGVIFSLYNYASNRREAYLLLQLFRTALQEEIKSKVEQPQDVVTGNPTVVRLVVRFYRNGRGQ
  SALQEILGKVIQDVLEDRSVSIHTDPVHIYKSWINQEEAQTGQRSHLPYDVTPEQALSHSEVQRRLDISL
  RSLLTLTEKFFVAISSSVDHIPYGMRYMAKVLKTTLEKKFPNATERDIYKVVGNLLYYRFLNPAVVAPDA
  FDIVAMAAGSTLAAPQRHALGAVAQLLQHAAAGQIFSGESRHLRVLNDYLEDLHLKFRKFICRACRVPEP
  EERFAIDEYSDMVAVAKPMVYITVGELIGTHRLLLEHQDQLAPGHQDPLHQLLEDLGEPPTISDLIGDNI
  ATDGHVDLSRLEVSLTLTNKFEGLETDADHNSNQSLLLSTKQMLADLIQFHPGESLEEILTSSAPREHEE
  AHRRLMCWRQACDTQKPEPLRRHHSLMAHSLLSLSEKQQRVLRNLRRLQGLGLVRASDCYQGLVDELAKD
  ICNQRRHRQRRKAEMLRLRTTLQGLDAKTIFYEEQGDYYNQYIQACLDHLAPKPKSSGKGKKQPSLHYTA
  AQLLEKGVLVEIEDLPVSHFRNVIFDITPGDEAGRFFVNAKFLGVDMEKFQLHYQDLLQLQYEGVAVMKL
  FNKAKVNVNLLIFLLNKKFLRK
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033484
• ORF Size: 4896 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033484.1, NP_001028656.1
• RefSeq Size: 5676 bp
• RefSeq ORF: 4899 bp
• Locus ID: 404710
• Cytogenetics: 3 38.78 cM