Atp2a2 (BC054531) Mouse Tagged ORF Clone

CAT#: MG211518

Atp2a2 (tGFP-tagged) - Mouse ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 (cDNA clone MGC:62264 IMAGE:6406916)

Product Images

Circular map for MG211518

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Atp2a2
• Synonyms: 9530097L16Rik; D5Wsu150e; mKIAA4195; SERCA2; Serca2a; SERCA2B
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211518 representing BC054531
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAACGCTCACACAAAGACCGTGGAGGAGGTGCTGGGCCACTTCGGGGTCAACGAGAGCACGGGGC
  TGAGCTTGGAGCAGGTCAAGAAGCTCAAGGAGAGATGGGGCTCCAACGAATTGCCGGCTGAAGAAGGAAA
  AACCTTGCTGGAACTTGTGATTGAGCAGTTTGAAGACTTACTAGTTAGAATTTTACTGCTGGCAGCATGT
  ATATCTTTCGTTTTGGCTTGGTTCGAGGAAGGGGAAGAAACGATTACAGCCTTTGTAGAGCCGTTTGTAA
  TTCTGCTTATCTTGGTAGCCAATGCAATCGTGGGTGTGTGGCAGGAAAGAAATGCTGAAAATGCAATAGA
  AGCTCTTAAGGAATATGAGCCTGAAATGGGCAAAGTGTATCGACAGGACAGAAAGAGTGTGCAACGAATT
  AAAGCTAAAGACATAGTTCCTGGTGATATAGTGGAAATTGCTGTTGGTGACAAAGTTCCTGCTGATATTA
  GATTGACATCCATCAAGTCTACAACTCTAAGAGTCGACCAGTCAATTCTTACAGGTGAATCTGTCTCCGT
  CATCAAGCATACTGACCCTGTCCCTGACCCCCGAGCTGTTAATCAAGACAAAAAGAACATGCTCTTTTCT
  GGTACAAACATTGCTGCTGGGAAAGCTATGGGAGTGGTGGTGGCAACTGGAGTTAATACTGAGATCGGCA
  AGATCCGGGATGAAATGGTGGCAACAGAACAGGAGAGAACACCCCTACAGCAGAAGCTAGACGAGTTTGG
  GGAGCAGCTTTCCAAAGTTATCTCCCTCATTTGCATTGCAGTCTGGATCATCAACATTGGGCATTTCAAT
  GACCCAGTTCATGGTGGCTCCTGGATCAGGGGTGCCATCTACTACTTTAAGATTGCCGTGGCCCTGGCTG
  TTGCCGCAATCCCTGAGGGTCTGCCTGCTGTCATCACCACCTGCTTAGCTCTTGGAACTCGTAGGATGGC
  AAAGAAAAATGCTATCGTTCGAAGTCTGCCTTCTGTGGAGACCCTTGGTTGTACTTCTGTTATCTGCTCA
  GATAAGACAGGCACACTTACCACAAACCAGATGTCCGTGTGCAGGATGTTCATTCTGGACAAAGTAGAAG
  GTGACACTTGTTCCCTTAATGAGTTCAGCATAACTGGATCCACATATGCACCAATTGGAGAAGTGCAAAA
  GGATGATAAGCCAGTGAAGTGCCATCAGTATGACGGGCTTGTAGAGTTAGCCACCATCTGTGCTCTGTGT
  AATGACTCTGCTTTGGATTATAATGAGGCAAAGGGTGTGTATGAGAAAGTTGGAGAAGCTACCGAGACTG
  CTCTCACGTGCCTGGTGGAGAAGATGAATGTATTTGATACTGAGCTGAAGGGGCTTTCTAAAATAGAGCG
  TGCAAACGCCTGCAACTCGGTCATAAAGCAGCTGATGAAGAAGGAGTTCACTCTGGAGTTTTCACGGGAT
  AGAAAATCAATGTCCGTCTATTGTACCCCAAACAAGCCAAGCCGGACATCCATGAGCAAGATGTTTGTGA
  AGGGGGCTCCAGAAGGTGTCATCGATAGGTGCACCCACATCCGAGTTGGAAGTACCAAGGTCCCCATGAC
  TCCTGGTGTCAAACAGAAGATTATGTCTGTCATTCGGGAGTGGGGCAGTGGCAGCGACACGCTACGGTGC
  CTGGCTCTGGCCACTCATGACAACCCACTGAAGAGAGAGGAGATGCACCTGGAAGACTCTGCTAACTTCA
  TCAAATACGAGACCAACCTGACTTTCGTCGGCTGTGTGGGCATGCTGGATCCTCCCAGGATTGAAGTAGC
  CTCTTCTGTGAAGCTGTGCCGGCAAGCAGGCATCCGGGTCATCATGATCACTGGAGACAACAAAGGCACC
  GCTGTGGCCATCTGTCGCCGCATTGGCATCTTTGGGCAGGATGAGGATGTGACATCAAAGGCTTTTACAG
  GGCGAGAGTTTGATGAATTAAGCCCTTCAGCCCAGAGAGATGCCTGCTTAAATGCCCGCTGTTTTGCTCG
  AGTTGAACCTTCCCACAAGTCTAAGATTGTTGAGTTCCTTCAGTCCTTTGATGAGATCACAGCTATGACT
  GGTGATGGTGTGAATGATGCTCCTGCTCTGAAGAAATCTGAAATCGGGATTGCCATGGGCTCAGGGACTG
  CAGTGGCTAAGACTGCTTCTGAGATGGTCCTGGCAGATGACAACTTCTCCACCATTGTGGCTGCTGTTGA
  GGAGGGGCGAGCCATCTACAACAACATGAAGCAGTTCATCCGCTACCTCATCTCATCCAACGTGGGGGAA
  GTGGTCTGTATCTTCCTGACGGCAGCCCTTGGGTTTCCTGAGGCTTTAATTCCTGTCCAGTTACTCTGGG
  TCAATCTGGTGACAGATGGTCTGCCTGCCACTGCGCTGGGGTTCAATCCTCCAGACCTGGACATCATGAA
  CAAACCCCCCCGGAACCCAAAAGAACCACTGATCAGCGGGTGGCTCTTTTTCCGTTACCTGGCTATTGGC
  TGTTATGTTGGCGCTGCCACCGTGGGTGCTGCTGCATGGTGGTTCATCGCTGCTGACGGCGGTCCAAGAG
  TCTCCTTCTACCAGCTGAGTCATTTCCTACAGTGTAAGGAGGACAACCCAGACTTCGATGGAGTGGATTG
  TGCAATCTTTGAGTCCCCATATCCAATGACAATGGCACTTTCTGTTCTAGTAACCATAGAGATGTGTAAT
  GCCCTCAACAGCTTGTCTGAAAACCAGTCTTTGCTGAGGATGCCCCCCTGGGAGAATATCTGGCTCGTGG
  GCTCCATCTGCTTGTCCATGTCACTTCACTTCTTGATCCTCTACGTGGAACCTTTGCCGCTCATTTTCCA
  GATCACACCGCTGAATCTGACCCAGTGGCTGATGGTGCTGAAAATCTCCTTGCCTGTGATCCTCATGGAT
  GAGACGCTCAAGTTTGTGGCCCGAAACTACCTGGAACAACCCGGTAAAGAGTGTGTGCAGCCTGCCACCA
  AATCTTCCTGCTCCCTGTCGGCATGCACCGATGGCATTTCCTGGCCGTTTGTGCTGCTCATTATGCCCCT
  GGTGGTCTGGGTCTACAGCACAGACACTAACTTTAGCGATATGTTCTGGTCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211518 representing BC054531
  Red=Cloning site Green=Tags(s)
  
  MENAHTKTVEEVLGHFGVNESTGLSLEQVKKLKERWGSNELPAEEGKTLLELVIEQFEDLLVRILLLAAC
  ISFVLAWFEEGEETITAFVEPFVILLILVANAIVGVWQERNAENAIEALKEYEPEMGKVYRQDRKSVQRI
  KAKDIVPGDIVEIAVGDKVPADIRLTSIKSTTLRVDQSILTGESVSVIKHTDPVPDPRAVNQDKKNMLFS
  GTNIAAGKAMGVVVATGVNTEIGKIRDEMVATEQERTPLQQKLDEFGEQLSKVISLICIAVWIINIGHFN
  DPVHGGSWIRGAIYYFKIAVALAVAAIPEGLPAVITTCLALGTRRMAKKNAIVRSLPSVETLGCTSVICS
  DKTGTLTTNQMSVCRMFILDKVEGDTCSLNEFSITGSTYAPIGEVQKDDKPVKCHQYDGLVELATICALC
  NDSALDYNEAKGVYEKVGEATETALTCLVEKMNVFDTELKGLSKIERANACNSVIKQLMKKEFTLEFSRD
  RKSMSVYCTPNKPSRTSMSKMFVKGAPEGVIDRCTHIRVGSTKVPMTPGVKQKIMSVIREWGSGSDTLRC
  LALATHDNPLKREEMHLEDSANFIKYETNLTFVGCVGMLDPPRIEVASSVKLCRQAGIRVIMITGDNKGT
  AVAICRRIGIFGQDEDVTSKAFTGREFDELSPSAQRDACLNARCFARVEPSHKSKIVEFLQSFDEITAMT
  GDGVNDAPALKKSEIGIAMGSGTAVAKTASEMVLADDNFSTIVAAVEEGRAIYNNMKQFIRYLISSNVGE
  VVCIFLTAALGFPEALIPVQLLWVNLVTDGLPATALGFNPPDLDIMNKPPRNPKEPLISGWLFFRYLAIG
  CYVGAATVGAAAWWFIAADGGPRVSFYQLSHFLQCKEDNPDFDGVDCAIFESPYPMTMALSVLVTIEMCN
  ALNSLSENQSLLRMPPWENIWLVGSICLSMSLHFLILYVEPLPLIFQITPLNLTQWLMVLKISLPVILMD
  ETLKFVARNYLEQPGKECVQPATKSSCSLSACTDGISWPFVLLIMPLVVWVYSTDTNFSDMFWS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC054531
• ORF Size: 3134 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC054531, AAH54531
• RefSeq Size: 5422 bp
• RefSeq ORF: 3134 bp
• Locus ID: 11938
• Cytogenetics: 5 F
• Gene Summary: This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Isoform SERCA2A is involved in the regulation of the contraction/relaxation cycle. Acts as a regulator of TNFSF11-mediated Ca(2+) signaling pathways via its interaction with TMEM64 which is critical for the TNFSF11-induced CREB1 activation and mitochondrial ROS generation necessary for proper osteoclast generation. Association between TMEM64 and SERCA2 in the ER leads to cytosolic Ca (2+) spiking for activation of NFATC1 and production of mitochondrial ROS, thereby triggering Ca (2+) signaling cascades that promote osteoclast differentiation and activation (PubMed:23395171).[UniProtKB/Swiss-Prot Function]