Ctnna2 (BC079648) Mouse Tagged ORF Clone

CAT#: MG211288

Ctnna2 (tGFP-tagged) - Mouse catenin (cadherin associated protein), alpha 2 (cDNA clone MGC:90598 IMAGE:6850068)

Product Images

Circular map for MG211288

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ctnna2
• Synonyms: AI481747; Catna; Catna2; cdf; chp
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211288 representing BC079648
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTTCGGCAACTTCACCTATTATTTTAAAATGGGATCCCAAAAGTTTGGAAATCCGGACACTCACAG
  TGGAAAGACTATTGGAGCCACTTGTGACACAGGTGACAACACTTGTCAACACAAGCAACAAAGGTCCGTC
  TGGTAAAAAGAAAGGGAGGTCAAAGAAAGCCCATGTGCTGGCAGCATCTGTAGAACAAGCTACTCAGAAC
  TTCCTGGAAAAGGGTGAACAGATCGCTAAGGAGAGCCAAGACCTCAAAGAAGAGTTAGTGGCTGCTGTAG
  AGGATGTGCGGAAGCAAGGTGAGACAATGCGGATTGCCTCCTCAGAGTTTGCAGATGACCCTTGCTCTTC
  TGTCAAGCGTGGCACCATGGTGCGTGCAGCACGGGCTCTGCTATCGGCTGTGACACGCTTGCTCATCCTG
  GCCGACATGGCAGATGTCATGAGGCTTTTATCGCATCTGAAAATTGTCGAGGAGGCCTTGGAAGCAGTCA
  AAAATGCCACAAATGAACAAGACCTTGCAAACCGATTTAAAGAGTTTGGGAAAGAGATGGTGAAACTGAA
  CTATGTAGCAGCAAGACGGCAGCAGGAGCTCAAGGACCCTCACTGTAGGGATGAGATGGCTGCAGCCCGT
  GGAGCCCTGAAGAAGAATGCCACCATGCTGTACACAGCCTCCCAAGCCTTCCTCCGGCATCCAGATGTTG
  CTGCTACAAGAGCCAACCGAGATTATGTATTTAAACAAGTCCAAGAGGCCATAGCTGGCATCTCCAGTGC
  TGCTCAGGCCACCTCCCCCACCGATGAAGCCAAAGGCCACACAGGCATCGGCGAGCTGGCTGCAGCCCTG
  AATGAGTTTGATAATAAGATCATCCTGGACCCCATGACATTCAGCGAGGCCAGGTTCCGACCATCCCTGG
  AGGAGAGACTGGAGAGCATCATCAGTGGGGCTGCTCTCATGGCAGATTCCTCCTGCACACGTGATGACCG
  CCGTGAGCGTATTGTGGCCGAGTGCAATGCAGTTCGACAGGCACTCCAGGACCTGCTAAGCGAGTACATG
  AATAACACTGGAAGGAAAGAGAAAGGAGACCCTCTCAACATTGCGATTGACAAGATGACCAAGAAAACAA
  GAGATCTGAGGAGACAGCTTCGGAAAGCTGTGATGGATCACATCTCAGATTCTTTCTTGGAAACCAATGT
  CCCCTTGCTGGTTCTCATTGAGGCTGCGAAGAGCGGGAATGAGAAGGAGGTGAAGGAATACGCCCAAGTT
  TTCCGTGAACATGCCAACAAGCTGGTGGAGGTTGCCAATTTGGCTTGTTCCATCTCCAACAATGAGGAAG
  GGGTGAAATTAGTCAGAATGGCAGCCACCCAGATTGACAGCCTGTGTCCCCAAGTCATTAATGCTGCCCT
  CACACTGGCTGCTCGGCCACAGAGTAAAGTTGCTCAGGACAACATGGATGTCTTCAAAGACCAGTGGGAA
  AAGCAAGTCCGTGTGCTCACTGAGGCAGTGGATGACATCACCTCTGTGGATGACTTCCTCTCTGTCTCAG
  AAAACCATATCTTGGAGGATGTGAACAAATGTGTGATTGCCCTGCAAGAGGGAGATGTGGACACACTGGA
  TCGCACAGCTGGGGCCATACGGGGCCGGGCAGCCCGGGTCATTCACATCATCAATGCAGAGATCGAGAAC
  TATGAAGCTGGGGTCTATACAGAGAAAGTGCTGGAAGCCACAAAATTGCTTTCAGAAACAGTGATGCCAC
  GCTTTGCTGAACAAGTTGAGGTGGCCATCGAAGCCCTGAGCGCCAATGTCCCTCAGCCATTCGAGGAGAA
  CGAGTTCATCGATGCCTCGCGCCTGGTGTATGACGGTGTTCGGGACATCAGAAAGGCTGTGCTGATGATC
  AGGACTCCAGAAGAGCTAGAAGATGATTCCGACTTTGAGCAAGAGGATTATGTGCGGAGTCGGACAAGTG
  TTCAGACAGAGGACGACCAGCTCATTGCTGGACAGAGTGCACGGGCCATCATGGCGCAACTACCACAGGA
  GGAGAAAGCAAAAATAGCTGAACAGGTGGAGATTTTCCACCAAGAAAAAAGCAAGCTGGATGCTGAAGTG
  GCCAAGTGGGATGACAGCGGCAATGACATCATTGTGCTGGCCAAGCAGATGTGTATGATCATGATGGAGA
  TGACAGACTTCACAAGAGGCAAAGGCCCACTGAAAAATACATCTGATGTCATTAATGCTGCCAAGAAGAT
  TGCAGAAGCAGGCTCTCGAATGGACAAATTAGCGCGCGCTGTGGCTGATCAGTGTCCTGATTCAGCATGT
  AAGCAGGATTTATTAGCCTACCTTCAGCGGATTGCTTTGTACTGCCATCAGCTTAACATCTGCAGCAAAG
  TGAAGGCCGAGGTTCAGAACCTAGGAGGAGAGCTCATTGTGTCAGGGACAGGAGTTCAGAGCACTTTCAC
  TACCTTTTATGAGGTAGATTGTGATGTCATAGATGGGGGCAGGGCTAGTCAACTTTCTACCCACCTCCCA
  ACCTGTGCTGAGGGAGCTCCAATCGGGAGTGGAAGCAGTGACTCCTCCATGCTGGACAGTGCTACATCAC
  TCATCCAGGCAGCCAAAAACCTGATGAATGCTGTTGTCCTCACGGTGAAAGCGTCTTATGTAGCCTCAAC
  TAAATACCAGAAGGTCTATGGAACAGCAGCTGTCAACTCTCCAGTTGTGTCTTGGAAGATGAAGGCTCCT
  GAAAAGAAGCCCCTTGTGAAGAGAGAAAAGCCTGAAGAATTCCAGACAAGAGTTAGACGGGGGTCTCAAA
  AGAAACACATTTCACCTGTGCAGGCTTTAAGTGAATTCAAGGCAATGGATTCCTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211288 representing BC079648
  Red=Cloning site Green=Tags(s)
  
  MTSATSPIILKWDPKSLEIRTLTVERLLEPLVTQVTTLVNTSNKGPSGKKKGRSKKAHVLAASVEQATQN
  FLEKGEQIAKESQDLKEELVAAVEDVRKQGETMRIASSEFADDPCSSVKRGTMVRAARALLSAVTRLLIL
  ADMADVMRLLSHLKIVEEALEAVKNATNEQDLANRFKEFGKEMVKLNYVAARRQQELKDPHCRDEMAAAR
  GALKKNATMLYTASQAFLRHPDVAATRANRDYVFKQVQEAIAGISSAAQATSPTDEAKGHTGIGELAAAL
  NEFDNKIILDPMTFSEARFRPSLEERLESIISGAALMADSSCTRDDRRERIVAECNAVRQALQDLLSEYM
  NNTGRKEKGDPLNIAIDKMTKKTRDLRRQLRKAVMDHISDSFLETNVPLLVLIEAAKSGNEKEVKEYAQV
  FREHANKLVEVANLACSISNNEEGVKLVRMAATQIDSLCPQVINAALTLAARPQSKVAQDNMDVFKDQWE
  KQVRVLTEAVDDITSVDDFLSVSENHILEDVNKCVIALQEGDVDTLDRTAGAIRGRAARVIHIINAEIEN
  YEAGVYTEKVLEATKLLSETVMPRFAEQVEVAIEALSANVPQPFEENEFIDASRLVYDGVRDIRKAVLMI
  RTPEELEDDSDFEQEDYVRSRTSVQTEDDQLIAGQSARAIMAQLPQEEKAKIAEQVEIFHQEKSKLDAEV
  AKWDDSGNDIIVLAKQMCMIMMEMTDFTRGKGPLKNTSDVINAAKKIAEAGSRMDKLARAVADQCPDSAC
  KQDLLAYLQRIALYCHQLNICSKVKAEVQNLGGELIVSGTGVQSTFTTFYEVDCDVIDGGRASQLSTHLP
  TCAEGAPIGSGSSDSSMLDSATSLIQAAKNLMNAVVLTVKASYVASTKYQKVYGTAAVNSPVVSWKMKAP
  EKKPLVKREKPEEFQTRVRRGSQKKHISPVQALSEFKAMDSF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC079648
• ORF Size: 2858 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC079648, AAH79648
• RefSeq Size: 4062 bp
• RefSeq ORF: 2858 bp
• Locus ID: 12386
• Cytogenetics: 6 33.54 cM
• Gene Summary: May function as a linker between cadherin adhesion receptors and the cytoskeleton to regulate cell-cell adhesion and differentiation in the nervous system (PubMed:12123610, PubMed:15034585). Required for proper regulation of cortical neuronal migration and neurite growth. It acts as negative regulator of Arp2/3 complex activity and Arp2/3-mediated actin polymerization. It thereby suppresses excessive actin branching which would impair neurite growth and stability (By similarity). Regulates morphological plasticity of synapses and cerebellar and hippocampal lamination during development. Functions in the control of startle modulation (PubMed:12089526).[UniProtKB/Swiss-Prot Function]