Mcm4 (NM_008565) Mouse Tagged ORF Clone

CAT#: MG210993

Mcm4 (tGFP-tagged) - Mouse minichromosome maintenance deficient 4 homolog (S

Product Images

Circular map for MG210993

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Mcm4
• Synonyms: 19G; AI325074; AU045576; Cdc21; mcdc21; Mcmd4; mKIAA4003
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210993 representing NM_008565
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGTCCCCGGCATCCACCCCGAGCCGCCGCAGCAGCCGACGCGGACGAGTCACCCCAACCCAGTCCC
  TTCGAAGTGAGGAAAGCAGGTCGTCACCCAATCGGAGACGTAGAGGCGAAGATTCTTCCACCGGAGAGCT
  ACTGCCAATGCCCACCTCACCAGGAGCCGACCTGCAGAGCCCACCTGCACAGAATGCCTTGTTTTCCAGC
  CCTCCTCAGATGCATTCTTTAGCTATTCCTTTGGACTTTGATGTTAGTTCACCATTGACATATGGCACTC
  CCAGCTCGCGAGTGGAAGGAACCCCAAGAAGTGGGGTGAGAGGCACACCTGTAAGGCAGAGGCCAGATCT
  GGGCTCAGCACGAAAGGGTTTGCAGGTGGATCTGCAGTCTGATGGCGCAGCAGCAGAAGACATCGTACCA
  AGTGAACAGTCTCTAGGCCAAAAGCTTGTGATTTGGGGAACAGATGTGAATGTGGCAACATGTAAAGAGA
  ATTTTCAGAGATTCCTTCAGTGTTTTACTGATCCTCTGGCCAAAGAAGAAGAAAATGTTGGCATAGATAT
  TACTCAACCTTTGTACATGCAACAACTTGGAGAGATTAATATTACAGGAGAGCCATTTTTAAATGTGAAC
  TGCGAACACATAAAATCATTTAGCAAAAATCTGTATAGACAGCTCATCTCCTACCCACAGGAGGTTATAC
  CAACCTTTGACATGGCTGTCAATGAGATCTTCTTTGACCGTTATCCTGACTCCATCTTAGAACATCAGAT
  TCAAGTCAGACCTTTTAATGCGTTGAAGACAAAGAGTATGAGAAACTTGAATCCAGAAGACATTGATCAG
  CTCATCACCATCAGTGGCATGGTCATCAGAACATCACAGCTGATTCCGGAGATGCAGGAGGCCTTTTTCC
  AATGCCAAGTCTCTGCCCACACCACCCGGGTGGAGATAGATCGAGGCAGAATTGCTGAGCCCTGCAGTTG
  TGTGCACTGCCACACTACCCACAGCATGGCACTGATCCACAACCGATCATTCTTCTCTGACAAGCAAATG
  ATCAAACTTCAAGAGTCTCCTGAAGACATGCCTGCTGGGCAGACACCTCACACTATTGTCCTTTTTGCCC
  ACAATGACCTTGTTGACAAGGTTCAACCAGGGGACAGAGTGAACGTCACAGGCATATATCGAGCAGTACC
  AATTCGAGTTAATCCAAGAGTGAGCAACGTGAAGTCTGTCTATAAAACCCACATTGATGTCATTCATTAT
  CGGAAAACGGATGCAAAACGTCTGCATGGCCTTGATGAAGAAGCAGAACAGAAACTTTTTTCAGAGAAAC
  GTGTGAAATTGCTTAAGGAACTTTCCAGGAAGCCAGATATTTATGAGCGGCTTGCTTCAGCCTTGGCTCC
  CAGCATTTATGAACATGAAGATATCAAAAAGGGAATCTTACTTCAGCTCTTTGGTGGAACAAGGAAGGAT
  TTCAGTCACACTGGGAGGGGTAAATTCCGTGCTGAGATCAACATCCTTCTGTGTGGGGACCCTGGCACCA
  GCAAGTCCCAGCTGCTACAGTATGTGTACAACCTGGTCCCCAGAGGCCAGTACACGTCTGGAAAAGGCTC
  CAGTGCGGTCGGCCTCACCGCCTATGTGATGAAAGACCCTGAGACCAGGCAGCTTGTCCTCCAGACAGGT
  GCCCTCGTCCTGAGTGACAATGGGATATGCTGCATCGATGAGTTTGACAAAATGAATGAAAGCACAAGGT
  CTGTGCTGCATGAGGTCATGGAACAGCAGACTCTGTCCATTGCAAAGGCTGGGATCATCTGTCAGCTCAA
  TGCGCGCACCTCTGTCCTGGCAGCAGCAAATCCTATTGAGTCTCAGTGGAATCCTAAAAAAACAACCATT
  GAAAATATCCAACTACCGCACACATTGTTGTCAAGGTTTGATCTCATTTTCCTCATGCTAGACCCTCAGG
  ATGAGGCATATGACCGGCGTCTAGCTCATCACCTGGTTTCATTGTACTACCAAAGTGAGGAGCAAGTGGA
  GGAGGAGTTCCTGGACATGGCCGTGCTGAAAGACTACATTGCATATGCCCATAGTACCATCATGCCCCGA
  CTGAGTGAGGAGGCCAGCCAGGCTCTCATTGAGGCTTATGTAAACATGAGGAAGATTGGGAGTAGCCGGG
  GGATGGTTTCTGCTTACCCTCGACAGCTAGAGTCATTAATTCGCTTAGCAGAAGCCCATGCTAAAGTAAG
  ATTTTCAAACAAAGTTGAAGCAATTGATGTGGAAGAGGCAAAACGCCTCCACCGGGAGGCTCTGAAGCAG
  TCTGCAACTGACCCTCGTACTGGCATTGTGGATATTTCTATTCTTACTACAGGAATGAGTGCCACTTCTC
  GTAAACGGAAAGAAGAATTAGCTGAAGCATTGAGAAAACTTATTTTATCTAAGGGTAAAACACCAGCCTT
  AAAGTACCAACAGCTGTTTGAGGATATTCGGGGACAGTCTGACACAGCAATTACCAAGGACATGTTTGAA
  GAAGCCCTGCGAGCTTTGGCTGATGATGATTTCCTAACAGTGACTGGGAAGACTGTCCGCCTGCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210993 representing NM_008565
  Red=Cloning site Green=Tags(s)
  
  MSSPASTPSRRSSRRGRVTPTQSLRSEESRSSPNRRRRGEDSSTGELLPMPTSPGADLQSPPAQNALFSS
  PPQMHSLAIPLDFDVSSPLTYGTPSSRVEGTPRSGVRGTPVRQRPDLGSARKGLQVDLQSDGAAAEDIVP
  SEQSLGQKLVIWGTDVNVATCKENFQRFLQCFTDPLAKEEENVGIDITQPLYMQQLGEINITGEPFLNVN
  CEHIKSFSKNLYRQLISYPQEVIPTFDMAVNEIFFDRYPDSILEHQIQVRPFNALKTKSMRNLNPEDIDQ
  LITISGMVIRTSQLIPEMQEAFFQCQVSAHTTRVEIDRGRIAEPCSCVHCHTTHSMALIHNRSFFSDKQM
  IKLQESPEDMPAGQTPHTIVLFAHNDLVDKVQPGDRVNVTGIYRAVPIRVNPRVSNVKSVYKTHIDVIHY
  RKTDAKRLHGLDEEAEQKLFSEKRVKLLKELSRKPDIYERLASALAPSIYEHEDIKKGILLQLFGGTRKD
  FSHTGRGKFRAEINILLCGDPGTSKSQLLQYVYNLVPRGQYTSGKGSSAVGLTAYVMKDPETRQLVLQTG
  ALVLSDNGICCIDEFDKMNESTRSVLHEVMEQQTLSIAKAGIICQLNARTSVLAAANPIESQWNPKKTTI
  ENIQLPHTLLSRFDLIFLMLDPQDEAYDRRLAHHLVSLYYQSEEQVEEEFLDMAVLKDYIAYAHSTIMPR
  LSEEASQALIEAYVNMRKIGSSRGMVSAYPRQLESLIRLAEAHAKVRFSNKVEAIDVEEAKRLHREALKQ
  SATDPRTGIVDISILTTGMSATSRKRKEELAEALRKLILSKGKTPALKYQQLFEDIRGQSDTAITKDMFE
  EALRALADDDFLTVTGKTVRLL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008565
• ORF Size: 2586 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008565.2, NP_032591.2
• RefSeq Size: 3360 bp
• RefSeq ORF: 2589 bp
• Locus ID: 17217
• UniProt ID: P49717
• Cytogenetics: 16 10.09 cM
• Gene Summary: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.[UniProtKB/Swiss-Prot Function]