Abcb6 (NM_023732) Mouse Tagged ORF Clone

CAT#: MG210924

Abcb6 (tGFP-tagged) - Mouse ATP-binding cassette, sub-family B (MDR/TAP), member 6 (Abcb6)

Product Images

Circular map for MG210924

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Abcb6
• Synonyms: 1200005B17Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210924 representing NM_023732
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGACTGTGGGCAACTACTGCGAGACCGAAGGGCCGGCGGGGCCCGCTTGGACTCAGAATGGCTTGA
  GTCCCTGCTTCTTCTTCACGCTAGTGCCCTCGACACTCCTGACTCTGGGGGTACTGGCCTTGGTGCTGGT
  TCTTCCCCGCAGGCGTCGGGAGGTGCCTGCTGGCCCAGAAGAGCTGTCTTGGGCGGCTGGCCCTCGGGTG
  GCCCCATATGTGCTGCAGCTGTTTCTGGCCACACTTCAGATGGCACTGCCCCTGGCTGGTCTGGCTGGCC
  GAGTGGGCACTGCTCGGGGGGTCCGTCTGCCAGGCTACCTACTGCTGGCTTCTGTGCTGGAGAGTCTGGC
  CAGCGTCTGTGGCTTGTGGCTGCTCGTGGTGGAACGGAGCCAGGCACGGCAGAGTCTGGCAATGGGCGTC
  TGGATGAAGTTCAGGCATAGCTTGGGTCTCCTGCTCCTCTGGACTGTGACATTTGCAGCCGAGAACTTGG
  CCCTAGTATCTTGGAACAGCCCGCAGTGGTGGTGGGCCAGAGCAGATCTGGGCCAGCAGGTTCAATTTGG
  CCTGTGGGTCCTGCGTTATGTGACCTCTGGAGGGCTATTTATCTTGGGGCTCTGGGCCCCAGGACTTCGC
  CCCCAGTCTTATACTCTACACGTTCATGAAGAAGACCAAGATGTAGGAGGGAACCAGGGCCGCTCAACAG
  ACCGACGATCCACCTGGAGAGACCTTGGCCGGAAGCTTCGACTGCTGAGTAGCTACTTGTGGCCTCGGGG
  AAGTCCATCACTACAGCTGATAGTGCTCATATGCCTGGGACTCATGGGGTTGGAACGAGCACTCAATGTG
  TTGGTCCCCATCTTCTATAGGGACATTGTGAACTTACTGACTGCGAAGGCTCCTTGGAGCTCCCTGGCCT
  GGACCGTTACCACCTATGTCTTCCTCAAGTTCCTCCAGGGGGGTGGCACTGGCAGTACAGGTTTTGTGAG
  CAACCTGCGAACCTTCCTATGGATCCGGGTGCAGCAGTTTACGTCCCGAGGTGTGGAGCTACGTCTCTTC
  TCTCACCTGCATGAACTCTCACTGCGCTGGCACCTGGGCCGGCGTACCGGGGAGGTGCTTCGGATCGTGG
  ACCGGGGAACATCTAGTGTCACAGGACTACTCAGCTACCTGGTGTTCAGCATCATCCCCACCCTGGCTGA
  CATCATCATTGGCATCATCTACTTCAGCATGTTCTTCAATGCCTGGTTTGGCCTCATCGTGTTCCTGTGC
  ATGAGTCTTTACCTCATCCTGACTATCGTGGTCACCGAGTGGAGAGCCAAGTTTCGCCGTGATATGAACA
  CACAGGAAAACGCCACCCGGGCACGAGCAGTAGACTCTCTGCTCAACTTTGAAACGGTGAAATACTATGG
  TGCCGAGGGTTATGAAGTAGACCGCTATCGAGAGGCCATCCTCAAGTTTCAGGGTTTGGAGTGGAAGTCA
  ACCGCCTCACTGGTTGTGCTGAATCAGACACAGAACCTGGTGATCGGGCTTGGCCTCCTTGCTGGCTCCT
  TGCTTTGCGCATACTTCGTCAGTGAGCAGAAGCTACAGGTTGGGGACTTTGTGCTTTTTGGCACTTACAT
  CACCCAACTGTACATGCCTCTCAACTGGTTTGGCACCTACTACAGGATGATCCAGACTAACTTCATTGAC
  ATGGAGAACATGTTTGACTTGCTGAAAGAGGAGACAGAAGTGAAGGATGTTCCTGGTGCAGGGCCCCTTC
  GTTTTCATAAGGGCCGGATTGAGTTTGAAAATGTGCACTTCAGCTACGCTGATGGGCAGGAGACCTTACA
  AGATGTGTCCTTCACTGTGATGCCTGGACAGACGGTGGCTCTGGTAGGTCCATCTGGGGCAGGAAAGAGC
  ACAATTTTGCGCCTGCTATTTCGCTTCTATGACATCAGCTCTGGCTGCATCCGAATAGATGGACAGGATA
  TTTCACAGGTGACCCAGATCTCTCTCCGATCTCACATTGGAGTTGTGCCCCAGGACACTGTCCTCTTCAA
  CGACACCATTGCTAACAATATCCGCTATGGCCGTGTCACAGCTGGGGACAGTGAGGTAGAGGCTGCTGCT
  CAGGCTGCAGGCATTCATGATGCCATCTTGTCTTTCCCTGAAGGGTATGAGACACAGGTCGGGGAGCGGG
  GTCTGAAGCTGAGTGGTGGCGAGAAGCAGCGAGTGGCCATCGCCCGCACCATCCTTAAGGCTCCTGACAT
  CATTCTGCTGGATGAGGCCACATCAGCACTGGATACATCTAATGAGAGAGCCATCCAGGCCTCTCTGGCC
  AAAGTCTGCACCAACCGCACCACCATCGTCATAGCACACAGGCTCTCAACTGTGGTCAATGCTGACCAGA
  TTCTGGTCATCAAGGACGGTTGCATCATAGAGAGAGGAAGGCACGAAGCCCTGCTGTCCCGAGGTGGCGT
  TTATGCCGAGATGTGGCAGCTGCAGCAGCAAGGACAAGAAACTGTCCCTGAAGAATCTAAACCTCAGGAC
  ACGGCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210924 representing NM_023732
  Red=Cloning site Green=Tags(s)
  
  MVTVGNYCETEGPAGPAWTQNGLSPCFFFTLVPSTLLTLGVLALVLVLPRRRREVPAGPEELSWAAGPRV
  APYVLQLFLATLQMALPLAGLAGRVGTARGVRLPGYLLLASVLESLASVCGLWLLVVERSQARQSLAMGV
  WMKFRHSLGLLLLWTVTFAAENLALVSWNSPQWWWARADLGQQVQFGLWVLRYVTSGGLFILGLWAPGLR
  PQSYTLHVHEEDQDVGGNQGRSTDRRSTWRDLGRKLRLLSSYLWPRGSPSLQLIVLICLGLMGLERALNV
  LVPIFYRDIVNLLTAKAPWSSLAWTVTTYVFLKFLQGGGTGSTGFVSNLRTFLWIRVQQFTSRGVELRLF
  SHLHELSLRWHLGRRTGEVLRIVDRGTSSVTGLLSYLVFSIIPTLADIIIGIIYFSMFFNAWFGLIVFLC
  MSLYLILTIVVTEWRAKFRRDMNTQENATRARAVDSLLNFETVKYYGAEGYEVDRYREAILKFQGLEWKS
  TASLVVLNQTQNLVIGLGLLAGSLLCAYFVSEQKLQVGDFVLFGTYITQLYMPLNWFGTYYRMIQTNFID
  MENMFDLLKEETEVKDVPGAGPLRFHKGRIEFENVHFSYADGQETLQDVSFTVMPGQTVALVGPSGAGKS
  TILRLLFRFYDISSGCIRIDGQDISQVTQISLRSHIGVVPQDTVLFNDTIANNIRYGRVTAGDSEVEAAA
  QAAGIHDAILSFPEGYETQVGERGLKLSGGEKQRVAIARTILKAPDIILLDEATSALDTSNERAIQASLA
  KVCTNRTTIVIAHRLSTVVNADQILVIKDGCIIERGRHEALLSRGGVYAEMWQLQQQGQETVPEESKPQD
  TA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_023732
• ORF Size: 2526 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023732.3
• RefSeq Size: 2946 bp
• RefSeq ORF: 2529 bp
• Locus ID: 74104
• UniProt ID: Q9DC29
• Cytogenetics: 1 C4
• Gene Summary: Binds heme and porphyrins and functions in their ATP-dependent uptake into the mitochondria. Plays a crucial role in heme synthesis.[UniProtKB/Swiss-Prot Function]