Ikbke (BC037446) Mouse Tagged ORF Clone

CAT#: MG210080

Ikbke (tGFP-tagged) - Mouse inhibitor of kappaB kinase epsilon (cDNA clone MGC:46849 IMAGE:3980145)

Product Images

Circular map for MG210080

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ikbke
• Synonyms: Ikki, IKK-i
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210080 representing BC037446
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGAGTACCACTAACTACCTGTGGCATACTGATGACCTGCTAGGGCAGGGGGCCACTGCCAGTGTGT
  ACAAGGCCCGAAACAAGAAATCCGGGGAGGTGGTTGCTGTAAAGGTCTTCAACTCAGCCAGCTATCGGCG
  ACCTCCTGAGGTTCAGGGAGGCAGCCGGCAGAAGGTGCTAATCATGGAGTACTGCTCCAGTGGGAGCCTG
  CTGAGCGTGCTGGAAGACCCTGAGAACACGTTCGGGCTTTCTGAAGAGGAGTTCCTAGTGGTGCTGCGCT
  GTGTGGTGGCTGGCATGAACCACCTGCGGGAGAATGGCATTGTCCATCGGGACATCAAACCTGGGAACAT
  CATGCGCCTGGTGGGCGAGGAGGGGCAGAGCATCTATAAGCTGTCTGACTTCGGGGCTGCCCGCAAGCTG
  GACGATGATGAGAAGTTTGTTTCTGTCTATGGTACAGAGGAATACCTGCACCCTGACATGTATGAGCGTG
  CAGTGCTGCGCAAACCCCAGCAAAAGGCATTTGGTGTGACTGTGGATCTCTGGAGTATTGGGGTGACCCT
  GTACCACGCAGCCACAGGCAGTCTGCCCTTCATCCCCTTCGGTGGGCCCCGGCGCAACAAAGAGATCATG
  TACAGAATCACCACAGAGAAGCCAGCCGGGGCCATTTCAGGGACTCAGAAGCAGGAAAATGGTCCCTTGG
  AGTGGAGCTACAGCCTCCCCATCACCTGTAGACTGTCCATGGGGCTGCAGAACCAGCTGGTGCCCATCCT
  GGCCAACATCCTGGAGGTGGAAGAGGATAAGTGCTGGGGCTTTGATCAGTTCTTCGCGGAGACCAGTGAC
  ATTCTGCAGCGAACGGTCATCCACGTCTTTTCCCTACCCCAGGCCGTTTTGCATCATGTCTACATCCACG
  CCCACAACACGATTGCCATCTTTTTGGAGGCTGTATATGAGCAGACCAACGTGACCCCCAAACACCAGGA
  GTACCTCTTCGAGGGTCACCCTTGTGTCCTTGAGCCAAGCCTCTCAGCCCAGCACATCGCCCACACAGCT
  GCCAGCAGCCCTCTAACTCTGTTCAGCATGTCCAGCGACACACCTAAGGGGCTGGCCTTCAGGGACCCTG
  CTCTGGATGTCCCAAAGTTCGTCCCTAAGGTTGACCTACAGGCCGATTACAGCACAGCTAAGGGGGTGCT
  GGGCGCTGGCTACCAGGCCCTGTGGCTGGCGCGGGTCCTGCTGGATGGACAGGCGTTGATGCTTCGGGGG
  TTACATTGGGTCCTGGAGGTGCTTCAGGACACGTGCCAGCAGACACTGGAGGTCACACGGACAGCCCTCC
  TCTACCTCGGCAGCAGCCTGGGCACTGAAAGGTTCAGCAGTGGATCGGGGATGCCTGACGTCCAGGAACG
  AAAGGAGGCCACAGAGCTAAGAACCAGGCTGCAGACTCTCTCAGAGATCCTGTCTAAATGTTCCCACAAT
  GTCACAGAAACCCAAAGGAGCCTGAGCTGTCTGGGTGAAGAGCTTTTAAAGAACCGGGACCAGATTCATG
  AGGATAACAAAAGTATCCAGAAGATTCAGTGTTGTTTGGACAAGATGCACTTCATCTACAAACAGTTCAA
  GAAATCCAGGATGAGGCCAGGGCTCAGCTACAATGAGGAGCAGATCCACAAGCTGGATAAGGTAAATTTC
  AGTCATCTAGCCAAGAGGCTGCTGCAGGTGTTCCAGGAGGAGTGTGTGCAGACGTATCAGGTGTCGCTGG
  TCACACACGGCAAGCGGATGAGGCAGGTGCAGAGGGCCCAGAACCACCTGCATCTCATTGGCCACTCTGT
  GGCCACCTGTAACTCGGAAGCCCGGGGAGCCCAGGAGAGTCTGAACAAGATCTTTGATCAGCTCCTTCTG
  GACAGAGCTTCCGAACAGGGAGCTGAGGTGTCACCGCAACCTATGGCTCCTCATCCCGGCCCTGATCCGA
  AGGACCTGGTCTTCCACATGCAGGAGCTTTGTAATGATATGAAGCTATTGGCCTTTGATCTCCAGGACAA
  CAACCGACTCATCGAACGGTTACATAGAGTTCCATCGGCACCAGATGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210080 representing BC037446
  Red=Cloning site Green=Tags(s)
  
  MQSTTNYLWHTDDLLGQGATASVYKARNKKSGEVVAVKVFNSASYRRPPEVQGGSRQKVLIMEYCSSGSL
  LSVLEDPENTFGLSEEEFLVVLRCVVAGMNHLRENGIVHRDIKPGNIMRLVGEEGQSIYKLSDFGAARKL
  DDDEKFVSVYGTEEYLHPDMYERAVLRKPQQKAFGVTVDLWSIGVTLYHAATGSLPFIPFGGPRRNKEIM
  YRITTEKPAGAISGTQKQENGPLEWSYSLPITCRLSMGLQNQLVPILANILEVEEDKCWGFDQFFAETSD
  ILQRTVIHVFSLPQAVLHHVYIHAHNTIAIFLEAVYEQTNVTPKHQEYLFEGHPCVLEPSLSAQHIAHTA
  ASSPLTLFSMSSDTPKGLAFRDPALDVPKFVPKVDLQADYSTAKGVLGAGYQALWLARVLLDGQALMLRG
  LHWVLEVLQDTCQQTLEVTRTALLYLGSSLGTERFSSGSGMPDVQERKEATELRTRLQTLSEILSKCSHN
  VTETQRSLSCLGEELLKNRDQIHEDNKSIQKIQCCLDKMHFIYKQFKKSRMRPGLSYNEEQIHKLDKVNF
  SHLAKRLLQVFQEECVQTYQVSLVTHGKRMRQVQRAQNHLHLIGHSVATCNSEARGAQESLNKIFDQLLL
  DRASEQGAEVSPQPMAPHPGPDPKDLVFHMQELCNDMKLLAFDLQDNNRLIERLHRVPSAPDV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC037446
• ORF Size: 2079 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC037446, AAH37446
• RefSeq Size: 2931 bp
• RefSeq ORF: 2081 bp
• Locus ID: 56489
• Cytogenetics: 1 E4
• Gene Summary: Serine/threonine kinase that plays an essential role in regulating inflammatory responses to viral infection, through the activation of the type I IFN, NF-kappa-B and STAT signaling. Also involved in TNFA and inflammatory cytokines, like Interleukin-1, signaling. Following activation of viral RNA sensors, such as RIG-I-like receptors, associates with DDX3X and phosphorylates interferon regulatory factors (IRFs), IRF3 and IRF7, as well as DDX3X. This activity allows subsequent homodimerization and nuclear translocation of the IRF3 leading to transcriptional activation of pro-inflammatory and antiviral genes including IFNB. In order to establish such an antiviral state, IKBKE forms several different complexes whose composition depends on the type of cell and cellular stimuli. Thus, several scaffolding molecules including IPS1/MAVS, TANK, AZI2/NAP1 or TBKBP1/SINTBAD can be recruited to the IKBKE-containing-complexes. Activated by polyubiquitination in response to TNFA and interleukin-1, regulates the NF-kappa-B signaling pathway through, at least, the phosphorylation of CYLD. Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. In addition, is also required for the induction of a subset of ISGs which displays antiviral activity, may be through the phosphorylation of STAT1 at 'Ser-708'. Phosphorylation of STAT1 at 'Ser-708' seems also to promote the assembly and DNA binding of ISGF3 (STAT1:STAT2:IRF9) complexes compared to GAF (STAT1:STAT1) complexes, in this way regulating the balance between type I and type II IFN responses. Protects cells against DNA damage-induced cell death. Also plays an important role in energy balance regulation by sustaining a state of chronic, low-grade inflammation in obesity, wich leads to a negative impact on insulin sensitivity. Phosphorylates AKT1.[UniProtKB/Swiss-Prot Function]