Foxp4 (BC057110) Mouse Tagged ORF Clone

CAT#: MG209934

Foxp4 (tGFP-tagged) - Mouse forkhead box P4 (cDNA clone MGC:61328 IMAGE:6417088)

Product Images

Circular map for MG209934

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Foxp4
• Synonyms: 2310007G05Rik, mFKHLA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209934 representing BC057110
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGTGGAGTCTGCATCGGAGACAATCAGGTCGGCTCCGTCTGGTCAGAACGGCGTGGGCAGCCTCT
  CTGCGCAGGCTGATGGCGGCGGTGGGGCCGGGACCGCGGGCACGGCCCCGGCTGCCGGTAGGGATGCCTC
  AGGCAGGGAAGCAGCCAGCGGAGGAGCCGACAGCAATGGCGAGATGAGCCCCGCGGAGCTTCTGCACTTC
  CAACAGCAACAGGCTCTCCAGGTGGCCCGGCAGTTCCTGTTGCAGCAAGCCTCCAGCCTGAACTCCCCGG
  GGAACAATGACAGTAAACAGTCGGCCTCTGCGGTGCAGGTGCCTGTGTCTGTGGCCATGATGTCCCAGCA
  GATGCTTACCCCCCAGCAGATGCAGCAGATCCTGTCGCCACCTCAGCTGCAGGCCTTGCTGCAGCAGCAA
  CAGGCTCTCATGCTCCAGCAGCTGCAGGAGTATTACAAGAAGCAGCAAGAGCAGCTCCACCTGCAGCTCC
  TCACCCAGCAGCAGGCTGGAAAACAGCAGCCCAAAGAGGCCCTGGGGAACAAGCAGCTGGCCTTCCAGCA
  GCAGCTCCTGCAGATGCAACAGTTGCAGCAACAGCACTTGCTCAACCTCCAGAGGCAGGGGCTGGTCAGC
  CTGCAGCCAAGCCAAGCCTCAGGGCCCCTCCAGGCCCTCCCGCAAGCCGTGTGCCCGACAGACCTGCCCC
  AGCTGTGGAAAGGCGAGGGTGCCCCGGGGCAGCCCGCTGAGGACAGCGGCAGGCAGGAGGGGCTGGACCT
  CGCCAGCACAGCTGTTACTGCTACCTCGTTTGCCAGCCCTCCCAAGGTCTCCCCGCCCCTCTCCCACCAC
  CCCTTACCCAACGGACAACCCACTGTGCTCACATCTCGGAGAGACAGCTCCTCCCATGAGGAGACCCCCA
  GTTCCCACCCTCTTTATGGACATGGGGAGTGCAAGTGGCCAGGCTGTGAGACCCTGTGTGAAGACCTGGG
  CCAGTTTATCAAACACCTCAACACAGAACACGCTTTGGATGACCGGAGCACGGCCCAATGCCGCGTGCAG
  ATGCAGGTGGTCCAACAGCTGGAGATCCAGCTGGCCAAGGAGAGTGAGCGGCTGCAGGCCATGATGGCAC
  ATCTGCACATGCGGCCTTCAGAGCCCAAGCCCTTCAGCCAGCCAGTGACCGTCTCCGCAGACCCGTTCCC
  GGATGGCCTCGTGCACCCCCCAACTTCAGCCGCTGCCCCCGTCACACCCCTGCGTCCCCCTGGCCTGGGC
  TCTGCCTCTTTGCACAGTGGGGGCCCTGCCCGCAGGAGAAGTAATGACAAATTCTGCTCCCCCATCTCCT
  CAGAGCTTGCCCAGAATCATGAGTTCTACAAGAACGCTGACGTCAGGCCCCCCTTCACCTACGCTTCCCT
  TATCCGCCAGGCCATTCTGGAAACTCCAGATCGGCAGCTGACGCTAAATGAGATTTATAACTGGTTCACC
  AGGATGTTCGCCTACTTCCGAAGAAACACGGCCACCTGGAAGAATGCCGTGCGCCACAACCTCAGCCTGC
  ACAAGTGTTTCGTCCGTGTGGAGAACGTCAAGGGGGCCGTGTGGACTGTGGATGAGCGGGAATATCAGAA
  ACGGAGACCGCCAAAGATGACAGGGAGTCCCACTCTGGTGAAGAACATGATCTCGGGCCTCAGTTACGGG
  GCACTTAACGCCAGCTACCAGGCTGCCCTGGCAGAGAGCAGCTTCCCTCTTCTCAGCAACCCCGGCATGC
  TGAACCCTGGCTCAGCCAGCAGCCTTCTGCCCCTCAGCCAGGAGGACCTGGGTGTCCCTGGGGAACCACT
  ACCCAGCAATGGGAGCAGTAGCCCGCCTCGTCTTTCCCCTCCTCAGTACAGCCACCAGATACAAGTCAAA
  GAGGAGCCTGCAGAGGCAGAGGAAGACCGACGGCCGGGGCCTCCCTTGGGCGCCCCCAACCCCAGCACTG
  TGGGGCCTCCAGAAGACAGGGACCTGGAGGAGGACCTGGGAGGAGAGGACATGTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209934 representing BC057110
  Red=Cloning site Green=Tags(s)
  
  MMVESASETIRSAPSGQNGVGSLSAQADGGGGAGTAGTAPAAGRDASGREAASGGADSNGEMSPAELLHF
  QQQQALQVARQFLLQQASSLNSPGNNDSKQSASAVQVPVSVAMMSQQMLTPQQMQQILSPPQLQALLQQQ
  QALMLQQLQEYYKKQQEQLHLQLLTQQQAGKQQPKEALGNKQLAFQQQLLQMQQLQQQHLLNLQRQGLVS
  LQPSQASGPLQALPQAVCPTDLPQLWKGEGAPGQPAEDSGRQEGLDLASTAVTATSFASPPKVSPPLSHH
  PLPNGQPTVLTSRRDSSSHEETPSSHPLYGHGECKWPGCETLCEDLGQFIKHLNTEHALDDRSTAQCRVQ
  MQVVQQLEIQLAKESERLQAMMAHLHMRPSEPKPFSQPVTVSADPFPDGLVHPPTSAAAPVTPLRPPGLG
  SASLHSGGPARRRSNDKFCSPISSELAQNHEFYKNADVRPPFTYASLIRQAILETPDRQLTLNEIYNWFT
  RMFAYFRRNTATWKNAVRHNLSLHKCFVRVENVKGAVWTVDEREYQKRRPPKMTGSPTLVKNMISGLSYG
  ALNASYQAALAESSFPLLSNPGMLNPGSASSLLPLSQEDLGVPGEPLPSNGSSSPPRLSPPQYSHQIQVK
  EEPAEAEEDRRPGPPLGAPNPSTVGPPEDRDLEEDLGGEDMS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC057110
• ORF Size: 2018 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC057110, AAH57110
• RefSeq Size: 4057 bp
• RefSeq ORF: 2018 bp
• Locus ID: 74123
• Cytogenetics: 17 C
• Gene Summary: Transcriptional repressor that represses lung-specific expression.[UniProtKB/Swiss-Prot Function]