Dars2 (NM_172644) Mouse Tagged ORF Clone

CAT#: MG209779

Dars2 (tGFP-tagged) - Mouse aspartyl-tRNA synthetase 2 (mitochondrial) (Dars2)

Product Images

Circular map for MG209779

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dars2
• Synonyms: 5830468K18Rik; aspRS
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209779 representing NM_172644
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATCTTGGTTTTTGGTTAAGTCGTCTATGCAGGGGTTTATCCAGACCCATCGGGAAGACCATGCGGC
  CAATCTGGGGTTCTCTTTCCAGAAATCTGGCGTTGAGTTCACAAAGAATTCCAGAATTCAGTAGCTTTGT
  TGCTCGGACCAACACATGTGGAGAGTTGCGTTCGTCCCACTTAGGCCAAGAAGTCACTCTGTGTGGATGG
  GTTCAGTACCGCAGGCAGAACACCTTCTTAGTCCTAAGAGATTGCCACGGCCTTGTTCAGATTCTCATTC
  CCCAGGATGAGTCAGCCGCATCTGTGAGGGGGATTTTGTGTGAAGCCCCTGTGGAGTCTGTGGTGAGAGT
  ATCTGGGACAGTAATCTCCCGTCCTCCAGGACAAGAGAATCCGAAAATGCCGACGGGTGAGATTGAAATC
  AAAGTTAAAACAGCTGAGCTCCTGAATGCCTGCAAAAAGCTGCCCTTTGAGATTAAAGACTTTGTGAAGA
  AAACAGAGGCGCTCCGTTTGCAGTATCGCTACTTAGATCTGCGCAGCTTCCAGATGCAGTACAACTTGCG
  GCTGAGGTCGCAGATGGTCATGAAAATGCGGGAATATCTCTGTAATCTGCACGGGTTTGTGGATATAGAA
  ACCCCAACCTTGTTTAAGAGGACCCCTGGAGGTGCCAAGGAGTTTTTAGTACCATCCAGGGAACCTGGAA
  AGTTTTATTCCCTCCCTCAAAGTCCTCAGCAATTTAAGCAGCTTCTGATGGTTGGAGGTTTAGACAGGTA
  TTTTCAGGTCGCTCGGTGTTACCGAGATGAAGGCTCAAGACCGGATAGACAGCCAGAGTTCACTCAGATT
  GACATAGAGATGTCCTTTGTAGAGCAGACTGGCATTCAGCGTTTAGTTGAGGGTTTGCTGCAGTATTCCT
  GGCCTGGTGATAAAGACCCTCTAGTGACTCCTTTCCCTTCCATGACTTTTGCTGAGGCACTGGCCACCTA
  TGGAACTGATAAGCCAGATACTCGCTTTGGGATGAAGATTGTGGATGTCAGTGATGTGTTCAGAAACACA
  GAGCTCAGATTCCTTCAGGATGCACTGGCTAAGCCCCAAGGAACTGTCAAAGCAATATGTGTTCATGACG
  GAGCGAAATACTTAAGGAAGGAAGACATTGAGTTCATTAGGAAGTTTGCAGTTCACCATTTCAGTCAGGA
  AGTCCTACCTATATTCCTGAATGCCAAGAAGAACTGGAGTTCGCCATTTGCTAAGTTCATAATGGAGGAG
  GAAAGACTGGAATTGGCCAGATCGATGGAGATACAAGAAGAAGACATAGTGTTACTGACTGCTGGGGAAC
  ATGAGAAAGCATGCTCTCTGTTGGGAAAGTTACGGCTAGAGTGTGCAGACCTTTTGGAGATGAGAGGAGC
  GGTGCTCCGAGACCCAGCAGTGTTCTCTTTTCTTTGGGTGGTTGACTTTCCACTCTTCCTTGCCAAGGAA
  GAGAGTCCCACAGAGCTGGAGTCAGCCCATCACCCATTTACTGCTCCTAACTCCAGTGACATTCACCTCC
  TCTATACTGAGCCGGAAAAGGTTCGTGGCCAACACTATGACTTGGTTTTAAACGGCAATGAGATAGGAGG
  TGGTTCTGCCCGAATCCATGATGCACAGCTACAGAGGTACATCCTGGAAACACTGCTAAAGGAAGATGTG
  AAATTGCTTTCCCATCTGCTTCAGGCTTTAGACTATGGGGCACCCCCACACGGAGGAATTGCCTTAGGGT
  TAGACAGACTGGTATGTCTTGTCACGGGAGCACCCAGCATCAGAGATGTCATAGCCTTCCCTAAATCCTA
  CCGAGGACAGGACCTTATGAGCAATGCTCCAGACTCCGTGTCTCCAGAAGAGCTGAAGCCTTACCATATC
  CACGTCTTGTGGCCAGCAGACTCAGAAGAGGAGAGTGCATCCGCCACACCTTCCAAACACTTGAGCTCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209779 representing NM_172644
  Red=Cloning site Green=Tags(s)
  
  MYLGFWLSRLCRGLSRPIGKTMRPIWGSLSRNLALSSQRIPEFSSFVARTNTCGELRSSHLGQEVTLCGW
  VQYRRQNTFLVLRDCHGLVQILIPQDESAASVRGILCEAPVESVVRVSGTVISRPPGQENPKMPTGEIEI
  KVKTAELLNACKKLPFEIKDFVKKTEALRLQYRYLDLRSFQMQYNLRLRSQMVMKMREYLCNLHGFVDIE
  TPTLFKRTPGGAKEFLVPSREPGKFYSLPQSPQQFKQLLMVGGLDRYFQVARCYRDEGSRPDRQPEFTQI
  DIEMSFVEQTGIQRLVEGLLQYSWPGDKDPLVTPFPSMTFAEALATYGTDKPDTRFGMKIVDVSDVFRNT
  ELRFLQDALAKPQGTVKAICVHDGAKYLRKEDIEFIRKFAVHHFSQEVLPIFLNAKKNWSSPFAKFIMEE
  ERLELARSMEIQEEDIVLLTAGEHEKACSLLGKLRLECADLLEMRGAVLRDPAVFSFLWVVDFPLFLAKE
  ESPTELESAHHPFTAPNSSDIHLLYTEPEKVRGQHYDLVLNGNEIGGGSARIHDAQLQRYILETLLKEDV
  KLLSHLLQALDYGAPPHGGIALGLDRLVCLVTGAPSIRDVIAFPKSYRGQDLMSNAPDSVSPEELKPYHI
  HVLWPADSEEESASATPSKHLSS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172644
• ORF Size: 1959 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172644.2, NP_766232.1
• RefSeq Size: 3582 bp
• RefSeq ORF: 1962 bp
• Locus ID: 226539
• UniProt ID: Q8BIP0
• Cytogenetics: 1 H2.1