Abhd16a (NM_178592) Mouse Tagged ORF Clone

CAT#: MG208850

Abhd16a (tGFP-tagged) - Mouse HLA-B associated transcript 5 (Bat5)

Product Images

Circular map for MG208850

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Abhd16a
• Synonyms: AI326074; Bat-5; Bat5; D17H6S82E; NG26
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208850 representing NM_178592
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAAGCTCCTGAGCTGCGTCCTCGGCCCCCGGCTCTACAAGATCTACCGGGAGAGGGACACGGACC
  GGGCCGCGTCCAGCGTCCCCGAGACTCCCACTGCCGTCCCCGCCGCCTCGTCCAGCTCCTGGGATACGTA
  TTATCAGCCTCGGGCCCTGGAGAAACATGCAGACAGCATCCTGGCACTGGCTTCAGTCTTCTGGTCCATC
  TCTTATTACTCCTCTCCCTTCGCCTTCTTCTACTTATACAGGAAAGGTTACTTGAGTTTGTCCAAAGTGG
  TGCCATTTTCTCACTATGCCGGGACGTTGCTGCTACTACTTGCAGGGGTGGCCTGCCTCCGAGGCATTGG
  CCGCTGGACCAACCCCCAGTATCGACAGTTCATCACCATCTTGGAAGCCACACATAGGAACCAGTCTGCA
  GAAAACAAGAGGCAGCTGGCCAACTATAACTTCGACTTCAGGAGCTGGCCAGTCGACTTCCACTGGGAGG
  AGCCCAGCAGCCGGAAGGGGTCCCGAGGTGGCCCTTCCCGCAGGGGTGTGGCCCTGCTTCGTCCAGAGCC
  TCTGCACCGGGGAACAGCAGACACGTTTCTCAACAGGGTCAAGAAGCTGCCCTGTCAGATCACCAGCTAT
  CTGGTGGCACACACCTTGGGGCGCCGGATGCTGTACCCAGGCTCCGTGTACCTGCTCCAGAAGGCCCTCA
  TGCCTGTGCTGCTGCAGGGCCAGGCCCGACTGGTGGAAGAGTGTAATGGGCGCCGTGCAAAACTGCTTGC
  CTGTGATGGTAATGAGATAGACACCATGTTTGTGGACCGACGGGGGACAGCTGAGCCCCAGGGGCAGAAG
  CTGGTCATCTGCTGTGAGGGGAATGCAGGATTCTATGAGGTGGGCTGTGTCTCCACACCCCTAGAAGCTG
  GCTATTCTGTCCTGGGCTGGAATCATCCAGGCTTTGCAGGAAGCACGGGGGTACCATTCCCACAGAACGA
  GGCCAATGCCATGGATGTGGTGGTTCAGTTTGCCATCCACCGCCTGGGCTTCCAGCCCCAGGACATTGTC
  ATCTACGCCTGGTCCATTGGAGGCTTCACTGCCACGTGGGCAGCCATGTCCTACCCAGACATCAGTGCTG
  TTATCCTGGACGCTTCCTTCGATGACTTGGTGCCCCTGGCCTTGAAGGTCATGCCGGACAGCTGGCGAGC
  CCTGGTCACCAGGACGGTGAGGCAGCATCTCAACCTCAACAACTCGGAGCAGCTGTGCAGGTTCCAGGGC
  CCCGTGCTGCTGGTCCGGAGAACCAAGGACGAGATCATCACCACCACGGTCCCTGAGGACATCATGTCCA
  ACAGAGGCAATGACCTCCTGCTGAAGCTCCTGCAGTTCCGGTATCCTCGAGTGATGGTGGAGGAGGGGCT
  CAGAGCTGTGAGGCAGTGGCTGGAGGCCTCTTCCCAGCTAGAGGAAGCCTCCATTTACAGTCGCTGGGAG
  GTGGAGGAGGACTGGTGTGTGTCTGTGCTTCGCTCCTACCAGGCAGAGCATGGGCCTGATTTCCCCTGGA
  GTGTGGGGGAGGACATGAGTGCAGATGGACGGAGGCAGCTGGCGCTGTTCCTGGCCCGGAAGCATCTACA
  CAACTTTGAGGCCACACACTGCACTCCACTCCCGGCCCAGCACTTCCAGATGCCCTGGCACCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208850 representing NM_178592
  Red=Cloning site Green=Tags(s)
  
  MAKLLSCVLGPRLYKIYRERDTDRAASSVPETPTAVPAASSSSWDTYYQPRALEKHADSILALASVFWSI
  SYYSSPFAFFYLYRKGYLSLSKVVPFSHYAGTLLLLLAGVACLRGIGRWTNPQYRQFITILEATHRNQSA
  ENKRQLANYNFDFRSWPVDFHWEEPSSRKGSRGGPSRRGVALLRPEPLHRGTADTFLNRVKKLPCQITSY
  LVAHTLGRRMLYPGSVYLLQKALMPVLLQGQARLVEECNGRRAKLLACDGNEIDTMFVDRRGTAEPQGQK
  LVICCEGNAGFYEVGCVSTPLEAGYSVLGWNHPGFAGSTGVPFPQNEANAMDVVVQFAIHRLGFQPQDIV
  IYAWSIGGFTATWAAMSYPDISAVILDASFDDLVPLALKVMPDSWRALVTRTVRQHLNLNNSEQLCRFQG
  PVLLVRRTKDEIITTTVPEDIMSNRGNDLLLKLLQFRYPRVMVEEGLRAVRQWLEASSQLEEASIYSRWE
  VEEDWCVSVLRSYQAEHGPDFPWSVGEDMSADGRRQLALFLARKHLHNFEATHCTPLPAQHFQMPWHL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178592
• ORF Size: 1674 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178592.2
• RefSeq Size: 1945 bp
• RefSeq ORF: 1677 bp
• Locus ID: 193742
• UniProt ID: Q9Z1Q2
• Cytogenetics: 17 18.59 cM
• Gene Summary: Phosphatidylserine (PS) lipase that mediates the hydrolysis of phosphatidylserine to generate lysophosphatidylserine (LPS) (PubMed:25580854). LPS constitutes a class of signaling lipids that regulates immunological and neurological processes (PubMed:25580854). Has no activity towards diacylglycerol, triacylglycerol or lysophosphatidylserine lipase (By similarity). Also has monoacylglycerol lipase activity, with preference for 1-(9Z,12Z-octadecadienoyl)-glycerol (1-LG) and 2-glyceryl-15-deoxy-Delta(12,14)-prostaglandin J2 (15d-PGJ(2)-G) (PubMed:25290914).[UniProtKB/Swiss-Prot Function]