Cdc23 (BC059013) Mouse Tagged ORF Clone

CAT#: MG207684

Cdc23 (tGFP-tagged) - Mouse CDC23 (cell division cycle 23, yeast, homolog) (cDNA clone MGC:69646 IMAGE:6813730)

Product Images

Circular map for MG207684

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cdc23
• Synonyms: 6030435O18; D18Ertd243e
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207684 representing BC059013
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATTCCAGATACCTGTCTGGAGAGAAGAAGAAGGATGACGAAACAGTTGATAGCCTAGGACCCCTAG
  AGAAGGGACAAGTCAAAAACGAGGCTCTCAGAGAACTGAGAGTGGAACTCAGCCGGAAGCACCAGGCTCG
  GGGACTTGATGGATTCGGCCTTTACCTGTATGGGGTGGTGCTTCGGAAACTGGACTTAGTGAAAGAGGCC
  ATTGATGTGTTTGTGGAGGCTACTCATGTTTTGCCTTTGCACTGGGGAGCCTGGTTAGAGCTGTGTAACC
  TGATCACAGACAAAGAGATGCTGAAGTTCCTGTCTTTGCCTGACACCTGGATGAAAGAGTTCTTCTTGGC
  TCACATATACACAGAACTGCAGCTGATAGAGGAGGCCCTGCAGAAGTATCAGCATCTCATTGATGTGGGC
  TTTTCCAAGAGCTCGTACATTGTTTCCCAAATTGCAGTTGCCTATCACAATATCAGAGATATCGACAAAG
  CCCTTTCTATTTTCAATGAGCTGAGGAAACAAGATCCTTACAGGATTGAAAATATGGACACATTCTCCAA
  CCTTCTCTATGTCAGGAGCATGAAGTCTGAATTGAGTTACCTGGCGCATAACCTCTGTGAAATTGATAAA
  TACCGAGTAGAAACATGCTGTGTAATTGGCAATTATTACAGCTTACGCTCCCAGCATGAGAAAGCAGCTT
  TGTATTTCCAGAGAGCCCTAAAGTTGAATCCTAGGTACCTTGGGGCCTGGACACTGATGGGGCACGAGTA
  CATGGAGATGAAGAACACGTCTGCAGCTATTCAGGCTTACCGACATGCCATTGAGGTCAACAAGAGAGAC
  TACAGAGCTTGGTATGGCCTTGGGCAGACTTACGAAATCCTTAAAATGCCATTTTATTGCCTTTATTATT
  ATAGACGGGCCCACCAGCTTCGTCCCAATGATTCTCGCATGCTGGTTGCCTTAGGAGAATGTTATGAGAA
  ACTCAATCAACTAGTGGAAGCCAAAAAGTGTTATTGGAGAGCATATGCCGTGGGAGACGTGGAGAAAATG
  GCTCTTGTGAAGCTGGCCAAGCTTCATGAACAGTTGACTGAGTCAGAGCAGGCTGCCCAGTGTTACATCA
  AATACATCCAAGATATCTATTCCTGTGGGGAGACCGTGGAGCACTTGGAGGAGAGCACAGCCTTCCGATA
  CCTGGCCCAGTACTATTTTAAGTGCAAGCTGTGGGATGAAGCTTCAACTTGTGCCCAAAAATGTTGTGCA
  TTCAATGATACCCGGGAAGAAGGTAAGGCCTTGCTCCGCCAGATCCTACAGCTGAGGAACCAAGGGGAGA
  CACCCACCTCTGACACGCCGGGTACCTTTTTCCTCCCTGCCTCACTGTCTGCAAACAACACTCCTACACG
  CAGAGTTTCTCCACTCAACCTGTCTTCAGTCACACCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207684 representing BC059013
  Red=Cloning site Green=Tags(s)
  
  MYSRYLSGEKKKDDETVDSLGPLEKGQVKNEALRELRVELSRKHQARGLDGFGLYLYGVVLRKLDLVKEA
  IDVFVEATHVLPLHWGAWLELCNLITDKEMLKFLSLPDTWMKEFFLAHIYTELQLIEEALQKYQHLIDVG
  FSKSSYIVSQIAVAYHNIRDIDKALSIFNELRKQDPYRIENMDTFSNLLYVRSMKSELSYLAHNLCEIDK
  YRVETCCVIGNYYSLRSQHEKAALYFQRALKLNPRYLGAWTLMGHEYMEMKNTSAAIQAYRHAIEVNKRD
  YRAWYGLGQTYEILKMPFYCLYYYRRAHQLRPNDSRMLVALGECYEKLNQLVEAKKCYWRAYAVGDVEKM
  ALVKLAKLHEQLTESEQAAQCYIKYIQDIYSCGETVEHLEESTAFRYLAQYYFKCKLWDEASTCAQKCCA
  FNDTREEGKALLRQILQLRNQGETPTSDTPGTFFLPASLSANNTPTRRVSPLNLSSVTP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC059013
• ORF Size: 1439 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC059013, AAH59013
• RefSeq Size: 3015 bp
• RefSeq ORF: 1439 bp
• Locus ID: 52563
• Cytogenetics: 18 18.69 cM
• Gene Summary: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity).[UniProtKB/Swiss-Prot Function]