Ero1a (NM_015774) Mouse Tagged ORF Clone

CAT#: MG207416

Ero1l (tGFP-tagged) - Mouse ERO1-like (S. cerevisiae) (Ero1l)

Product Images

Circular map for MG207416

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ero1a
• Synonyms: Er; ERO1-L; Ero1l
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207416 representing NM_015774
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCGCGCCTGGGGCTTGCTCGTTGGACTCCTGGGCGTCGTGTGGCTGCTGCGCTTGGGCCACGGCG
  AGGAGCGGCGGCCGGAGACAGCGGCACAGCGCTGCTTCTGCCAGGTTAGTGGTTACCTGGACGACTGTAC
  CTGTGATGTCGAGACCATCGATAAGTTTAATAACTACAGACTTTTCCCAAGACTACAAAAGCTTCTTGAA
  AGTGACTACTTTAGATATTACAAGGTGAACTTGAAGAAGCCTTGTCCTTTCTGGAATGACATCAACCAGT
  GTGGAAGAAGAGACTGTGCCGTCAAACCCTGCCATTCTGATGAAGTTCCTGATGGAATTAAGTCTGCGAG
  CTACAAGTATTCTGAGGAAGCCAACCGCATTGAAGAAGGTGAGCAAGCTGAGCGACTTGGAGCCGTGGAT
  GAGTCTCTGAGTGAGGAGACCCAGAAAGCTGTACTTCAGTGGACCAAGCATGATGATTCGTCAGACAGCT
  TCTGCGAAATTGACGATATACAGTCCCCCGATGCTGAGTATGTGGACTTACTCCTTAACCCTGAGCGCTA
  CACAGGCTACAAGGGGCCAGACGCTTGGAGGATATGGAGTGTCATCTATGAAGAAAACTGTTTTAAGCCA
  CAGACAATTCAAAGGCCTTTGGCTTCTGGGCGAGGAAAAAGTAAAGAGAACACATTTTACAACTGGCTAG
  AAGGCCTCTGTGTAGAAAAGAGAGCATTCTACAGACTTATATCTGGCCTGCACGCAAGCATTAATGTGCA
  TTTGAGTGCAAGGTATCTTTTACAAGATACTTGGCTGGAAAAGAAATGGGGTCACAATGTCACAGAGTTC
  CAGCAGCGCTTTGATGGGATTCTGACTGAAGGAGAAGGCCCACGAAGGCTGAGGAACTTGTACTTCCTGT
  ACCTGATAGAGTTAAGGGCTCTCTCCAAAGTGCTTCCATTTTTTGAGCGTCCAGATTTTCAGCTCTTCAC
  TGGGAATAAAGTTCAGGATGCAGAAAACAAAGCGTTACTTCTGGAGATACTTCATGAAATCAAGTCATTT
  CCTTTGCACTTCGATGAGAATTCTTTTTTTGCTGGGGATAAAAACGAAGCACATAAACTAAAGGAGGACT
  TCCGGCTACACTTTAGGAACATTTCAAGAATCATGGACTGTGTTGGCTGCTTCAAGTGCCGCCTGTGGGG
  CAAGCTTCAGACGCAGGGGCTGGGCACTGCTCTGAAGATCTTGTTTTCCGAAAAACTGATCGCAAATATG
  CCGGAAAGCGGACCAAGTTATGAGTTCCAGCTAACCAGACAAGAAATAGTATCACTGTTTAATGCATTTG
  GAAGGATTTCCACAAGTGTGAGAGAACTAGAGAACTTCAGGCACTTGTTACAGAATGTTCAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207416 representing NM_015774
  Red=Cloning site Green=Tags(s)
  
  MGRAWGLLVGLLGVVWLLRLGHGEERRPETAAQRCFCQVSGYLDDCTCDVETIDKFNNYRLFPRLQKLLE
  SDYFRYYKVNLKKPCPFWNDINQCGRRDCAVKPCHSDEVPDGIKSASYKYSEEANRIEEGEQAERLGAVD
  ESLSEETQKAVLQWTKHDDSSDSFCEIDDIQSPDAEYVDLLLNPERYTGYKGPDAWRIWSVIYEENCFKP
  QTIQRPLASGRGKSKENTFYNWLEGLCVEKRAFYRLISGLHASINVHLSARYLLQDTWLEKKWGHNVTEF
  QQRFDGILTEGEGPRRLRNLYFLYLIELRALSKVLPFFERPDFQLFTGNKVQDAENKALLLEILHEIKSF
  PLHFDENSFFAGDKNEAHKLKEDFRLHFRNISRIMDCVGCFKCRLWGKLQTQGLGTALKILFSEKLIANM
  PESGPSYEFQLTRQEIVSLFNAFGRISTSVRELENFRHLLQNVH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015774
• ORF Size: 1392 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015774.2, NP_056589.1
• RefSeq Size: 3139 bp
• RefSeq ORF: 1395 bp
• Locus ID: 50527
• UniProt ID: Q8R180
• Cytogenetics: 14 C1
• Gene Summary: This gene encodes a member of the endoplasmic reticulum oxidoreductin family. The encoded protein is localized to the endoplasmic reticulum and promotes the formation of disulfide bonds by oxidizing protein disulfide isomerase. This gene may play a role in endoplasmic reticulum stress-induced apoptosis and the cellular response to hypoxia. [provided by RefSeq, Feb 2011]