Gmppa (BC008116) Mouse Tagged ORF Clone

CAT#: MG206677

Gmppa (tGFP-tagged) - Mouse GDP-mannose pyrophosphorylase A (cDNA clone MGC:7719 IMAGE:3498021)

Product Images

Circular map for MG206677

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gmppa
• Synonyms: 1810012N01Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206677 representing BC008116
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCAAAGCTGTGATTCTCATTGGAGGCCCCCAGAAGGGGACTCGCTTCAGGCCTTTGTCTTTTGAGG
  TGCCCAAACCTCTGTTTCCTGTGGCAGGCGTTCCCATGATCCAGCACCATATAGAAGCCTGTGCCCAGGT
  CCCAGGGATGCAGGAGATTCTTCTCATTGGCTTCTACCAGCCTGATGAGGCCCTCACCCAGTTCCTGGAA
  GCTGCCCAGCAGGAGTTTAACCTTCCAGTCAGGTACCTGCAGGAGTTTGCCCCCCTCGGCACAGGGGGTG
  GCCTCTACCATTTTCGGGACCAGATCCTGGCTGGGGCACCTGAGGCCTTCTTCGTGCTCAATGCTGACGT
  CTGCTCTGACTTCCCCTTGAGCGCCATGTTGGAGGCTCACAGGCGCCAGCGCCACCCTTTCTTACTCCTT
  GGCACCACGGCTAACAGGACACAATCCCTCAACTACGGCTGCATCGTTGAGAATCCACAGACTCATGAGG
  TTCTGCACTATGTGGAGAAACCCAGCACCTTTATCAGTGACATCATCAACTGTGGCATCTACCTTTTCTC
  CCCAGAAGCCCTGAAGCCTCTCCGGGATGTTTTCCAGCGTAACCAACAGGATGGGCAACTGGAGGAGTCC
  CCAGGCTCGTGGCCTGGCGCAGGGACCATCCGACTAGAACAGGACGTGTTCTCAGCCCTGGCTGGGCAGG
  GCCAAATCTACGTGCACCTCACCGATGGTATCTGGAGCCAGATCAAGTCTGCAGGCTCAGCCCTTTATGC
  CTCCCGCCTCTATCTGGGGCGATACCAGATCACTCACCCAGAACGGCTAGCCAGACACACTCCAGGGGGC
  CCACGCATCAGAGGAAATGTTTACATCCATCCAACCGCTAAAGTGGCCCCATCAGCTGTGCTGGGCCCCA
  ATGTCTCCATTGGGAAGGGGGTGACCATAGGCGAGGGTGTGCGTCTGCGAGAGAGCATTGTCCTCCATGG
  AGCCACTTTGCAGGAACACACCTGCGTCCTTCACAGCATTGTGGGCTGGGGGAGCACTGTGGGGCGCTGG
  GCCCGTGTAGAGGGGACTCCCAATGACCCCAATCCGAATGACCCCCGAGCTCGCATGGACAGTGAGAGCC
  TCTTCAAAGATGGAAAGCTGCTTCCTGCCATCACTATCCTGGGCTGCCGTGTTCGGATCCCTGCCGAGGT
  GCTCATCTTGAACTCGATTGTTCTACCACATAAGGAGCTAAGTCGAAGCTTCACCAACCAAATCATCCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206677 representing BC008116
  Red=Cloning site Green=Tags(s)
  
  MLKAVILIGGPQKGTRFRPLSFEVPKPLFPVAGVPMIQHHIEACAQVPGMQEILLIGFYQPDEALTQFLE
  AAQQEFNLPVRYLQEFAPLGTGGGLYHFRDQILAGAPEAFFVLNADVCSDFPLSAMLEAHRRQRHPFLLL
  GTTANRTQSLNYGCIVENPQTHEVLHYVEKPSTFISDIINCGIYLFSPEALKPLRDVFQRNQQDGQLEES
  PGSWPGAGTIRLEQDVFSALAGQGQIYVHLTDGIWSQIKSAGSALYASRLYLGRYQITHPERLARHTPGG
  PRIRGNVYIHPTAKVAPSAVLGPNVSIGKGVTIGEGVRLRESIVLHGATLQEHTCVLHSIVGWGSTVGRW
  ARVEGTPNDPNPNDPRARMDSESLFKDGKLLPAITILGCRVRIPAEVLILNSIVLPHKELSRSFTNQIIL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC008116
• ORF Size: 1262 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC008116, AAH08116
• RefSeq Size: 1500 bp
• RefSeq ORF: 1262 bp
• Locus ID: 69080
• Cytogenetics: 1 C4
• Gene Summary: May serve as a regulatory subunit and allow allosteric feedback inhibition of GMPPB by GDP-mannose.[UniProtKB/Swiss-Prot Function]