Ass1 (BC002074) Mouse Tagged ORF Clone

CAT#: MG206472

Ass1 (tGFP-tagged) - Mouse argininosuccinate synthetase 1 (cDNA clone MGC:6218 IMAGE:3491910)

Product Images

Circular map for MG206472

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ass1
• Synonyms: AA408052; ASS; Ass-1; fold
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206472 representing BC002074
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCAGCAAGGGCTCTGTGGTTCTGGCCTACAGTGGTGGCCTGGACACCTCCTGCATCCTCGTGTGGC
  TGAAGGAACAAGGCTATGATGTCATCGCCTACCTGGCCAACATTGGCCAGAAGGAAGACTTTGAGGAAGC
  CAGGAAGAAGGCGCTGAAGCTTGGGGCCAAAAAGGTGTTCATTGAGGATGTGAGCAAGGAATTTGTGGAA
  GAGTTCATCTGGCCTGCTGTCCAGTCCAGTGCACTCTACGAGGACCGCTATCTCCTGGGCACCTCTCTCG
  CCAGGCCTTGCATAGCTCGCAGACAGGTGGAGATTGCCCAGCGTGAAGGGGCCAAGTATGTGTCTCACGG
  CGCCACGGGAAAGGGGAATGACCAGGTCCGCTTTGAGCTCACCTGCTACTCACTGGCACCCCAGATTAAG
  GTCATCGCTCCCTGGAGGATGCCTGAGTTTTACAACCGGTTCAAGGGCCGAAATGATCTGATGGAGTATG
  CAAAGCAACACGGAATCCCCATCCCTGTCACCCCCAAGAGCCCCTGGAGTATGGATGAAAACCTCATGCA
  CATCAGCTATGAGGCTGGGATCCTGGAAAACCCCAAGAATCAAGCACCTCCGGGTCTCTACACAAAAACT
  CAGGACCCTGCCAAAGCACCCAACAGCCCAGATGTCCTTGAGATAGAATTCAAAAAAGGGGTCCCTGTGA
  AGGTGACCAACATCAAAGATGGCACAACCCGCACCACATCCCTGGAACTCTTCATGTACCTGAACGAAGT
  TGCGGGCAAGCACGGAGTGGGTCGCATTGACATCGTGGAGAACCGCTTCATTGGAATGAAGTCCCGAGGT
  ATCTACGAGACCCCAGCAGGGACCATCCTTTACCACGCTCATTTAGACATAGAGGCCTTCACGATGGATC
  GGGAAGTACGCAAAATCAAGCAGGGCCTGGGCCTCAAATTCGCAGAGCTCGTATACACAGGTTTCTGGCA
  CAGCCCTGAATGTGAATTTGTTCGCCACTGTATCCAGAAGTCCCAGGAGCGGGTAGAAGGGAAGGTGCAG
  GTGTCTGTCTTCAAGGGCCAAGTGTACATCCTCGGTCGGGAGTCTCCACTTTCACTCTACAATGAAGAGC
  TGGTGAGCATGAACGTACAGGGCGACTATGAGCCCATCGACGCCACTGGCTTCATCAATATCAACTCGCT
  CAGGCTGAAGGAGTACCATCGCCTTCAGAGCAAGGTCACTGCCAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206472 representing BC002074
  Red=Cloning site Green=Tags(s)
  
  MSSKGSVVLAYSGGLDTSCILVWLKEQGYDVIAYLANIGQKEDFEEARKKALKLGAKKVFIEDVSKEFVE
  EFIWPAVQSSALYEDRYLLGTSLARPCIARRQVEIAQREGAKYVSHGATGKGNDQVRFELTCYSLAPQIK
  VIAPWRMPEFYNRFKGRNDLMEYAKQHGIPIPVTPKSPWSMDENLMHISYEAGILENPKNQAPPGLYTKT
  QDPAKAPNSPDVLEIEFKKGVPVKVTNIKDGTTRTTSLELFMYLNEVAGKHGVGRIDIVENRFIGMKSRG
  IYETPAGTILYHAHLDIEAFTMDREVRKIKQGLGLKFAELVYTGFWHSPECEFVRHCIQKSQERVEGKVQ
  VSVFKGQVYILGRESPLSLYNEELVSMNVQGDYEPIDATGFININSLRLKEYHRLQSKVTAK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC002074
• ORF Size: 1238 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC002074, AAH02074
• RefSeq Size: 1645 bp
• RefSeq ORF: 1238 bp
• Locus ID: 11898
• Cytogenetics: 2 21.81 cM
• Gene Summary: One of the enzymes of the urea cycle, the metabolic pathway transforming neurotoxic amonia produced by protein catabolism into inocuous urea in the liver of ureotelic animals. Catalyzes the formation of arginosuccinate from aspartate, citrulline and ATP and together with ASL it is responsible for the biosynthesis of arginine in most body tissues.[UniProtKB/Swiss-Prot Function]