Jmjd6 (NM_033398) Mouse Tagged ORF Clone

CAT#: MG206341

Jmjd6 (tGFP-tagged) - Mouse phosphatidylserine receptor (Ptdsr)

Product Images

Circular map for MG206341

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Jmjd6
• Synonyms: 5730436I23Rik; D11Ertd195e; mKIAA0585; PSR; PtdSerR; Ptdsr
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206341 representing NM_033398
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCACAAGAGCAAGAAGCGCATCCGCGAGGCCAAGCGAAGTGCGCGGCCGGAGCTCAAGGACTCGC
  TCGACTGGACCCGGCACAACTACTACGAGAGCTACCCGCTGAACCCCGCGGCCGTGTCGGATAACGTGGA
  GAGAGCTGATGCCTTACAGCTGTCGGTGAAAGAGTTCGTGGAGCGCTACGAGAGGCCTTACAAGCCCGTG
  GTTCTGCTCAATGCACAAGAGGGCTGGTCCGCACAGGAGAAATGGACTCTGGAGCGCCTCAAAAGGAAAT
  ACCGGAACCAGAAGTTCAAGTGCGGCGAGGATAATGACGGCTACTCGGTGAAGATGAAGATGAAGTACTA
  CATCGAGTACATGGAGAGCACCCGCGATGACAGTCCCCTTTACATCTTCGATAGCAGCTATGGCGAACAC
  CCCAAAAGAAGGAAACTTTTGGAAGACTACAAGGTGCCCAAGTTTTTCACAGATGATCTTTTCCAATACG
  CGGGGGAGAAACGCAGACCCCCTTACAGGTGGTTTGTGATGGGGCCACCGCGTTCTGGAACTGGGATTCA
  CATCGACCCTCTGGGGACCAGTGCCTGGAATGCCTTAGTTCAGGGTCACAAGCGGTGGTGCCTCTTCCCA
  ACAAACACACCCAGAGAACTCATCAAGGTGACCCGAGAAGAAGGAGGGAACCAACAGGATGAAGCAATTA
  CCTGGTTTAATGTCATCTATCCCCGGACACAGCTTCCAACCTGGCCACCTGAATTCAAACCCCTGGAGAT
  ATTACAGAAACCAGGAGAAACTGTCTTTGTACCAGGGGGCTGGTGGCATGTTGTCCTCAACCTTGACACC
  ACCATTGCCATCACCCAGAACTTTGCCAGCAGCACCAACTTCCCTGTTGTGTGGCACAAGACGGTAAGAG
  GGAGACCAAAGTTATCAAGGAAGTGGTATAGGATCTTGAAACAGGAGCACCCTGAGCTGGCAGTCCTGGC
  CGACGCAGTTGACCTCCAGGAGTCCACAGGCATTGCCTCTGACAGCTCCAGCGACTCTTCTAGCTCCTCT
  AGTTCCAGCTCGTCAGACTCGGACTCAGAGTGTGAATCTGGGTCAGAAGGTGATGGGACGACACACCGCA
  GGAAGAAGAGGAGAACCTGCAGCATGGTGGGAAATGGGGACACTACCTCCCAGGATGACTGTGTGAGCAA
  AGAGCGCAGCTCCTCCAGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206341 representing NM_033398
  Red=Cloning site Green=Tags(s)
  
  MNHKSKKRIREAKRSARPELKDSLDWTRHNYYESYPLNPAAVSDNVERADALQLSVKEFVERYERPYKPV
  VLLNAQEGWSAQEKWTLERLKRKYRNQKFKCGEDNDGYSVKMKMKYYIEYMESTRDDSPLYIFDSSYGEH
  PKRRKLLEDYKVPKFFTDDLFQYAGEKRRPPYRWFVMGPPRSGTGIHIDPLGTSAWNALVQGHKRWCLFP
  TNTPRELIKVTREEGGNQQDEAITWFNVIYPRTQLPTWPPEFKPLEILQKPGETVFVPGGWWHVVLNLDT
  TIAITQNFASSTNFPVVWHKTVRGRPKLSRKWYRILKQEHPELAVLADAVDLQESTGIASDSSSDSSSSS
  SSSSSDSDSECESGSEGDGTTHRRKKRRTCSMVGNGDTTSQDDCVSKERSSSR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033398
• ORF Size: 1209 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033398.1
• RefSeq Size: 1688 bp
• RefSeq ORF: 1212 bp
• Locus ID: 107817
• UniProt ID: Q9ERI5
• Cytogenetics: 11 81.49 cM
• Gene Summary: This gene encodes a nuclear protein with a JmjC domain. JmjC domain-containing proteins are predicted to function as protein hydroxylases or histone demethylases. This protein functions in differentiation of multiple tissues during development, and in anti-inflammatory cytokine signaling. It was first identified as a putative phosphatidylserine receptor involved in phagocytosis of apoptotic cells; however, subsequent studies have indicated that this protein does not directly function in the clearance of apoptotic cells, and questioned whether it is a true phosphatidylserine receptor. [provided by RefSeq, Jul 2008]