Cxcr5 (NM_007551) Mouse Tagged ORF Clone

CAT#: MG205790

Cxcr5 (tGFP-tagged) - Mouse Burkitt lymphoma receptor 1 (Blr1)

Product Images

Circular map for MG205790

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cxcr5
• Synonyms: Blr1; CXC-R5; CXCR-5; Gpcr6; MDR15
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205790 representing NM_007551
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTACCCACTAACCCTGGACATGGGCTCCATCACATACAATATGGATGACCTGTACAAGGAACTGG
  CCTTCTACAGTAACAGCACGGAGATTCCCCTACAGGACAGTAACTTCTGCTCTACAGTCGAGGGACCCTT
  ACTGACGTCCTTTAAGGCGGTATTCATGCCTGTGGCCTACAGCCTCATCTTCCTCCTGGGTATGATGGGA
  AACATCCTGGTGCTGGTAATCCTGGAGAGGCACCGGCACACTCGGAGCTCAACCGAGACCTTCCTGTTCC
  ACCTCGCAGTAGCCGACCTTCTCTTAGTCTTCATCCTGCCTTTTGCAGTGGCTGAGGGCTCTGTGGGTTG
  GGTCCTAGGGACCTTCCTCTGCAAAACTGTGATCGCTCTGCACAAGATCAATTTCTACTGCAGCAGCCTG
  CTGCTGGCCTGTATAGCTGTAGACCGGTACCTAGCCATCGTCCATGCTGTTCACGCCTACCGCCGCCGTC
  GACTCCTCTCCATCCACATCACCTGCACGGCCATTTGGCTGGCCGGCTTCCTGTTCGCCTTACCGGAACT
  CCTCTTTGCCAAGGTTGGCCAACCTCATAACAACGACTCCTTACCACAGTGCACCTTCTCCCAGGAAAAC
  GAAGCGGAAACTAGAGCCTGGTTCACCTCCCGTTTCCTCTACCACATCGGGGGCTTCCTACTACCGATGC
  TTGTGATGGGATGGTGTTACGTGGGCGTGGTCCACAGGCTACTGCAGGCCCAGCGGCGCCCTCAGCGGCA
  GAAGGCGGTCAGGGTGGCCATTTTAGTGACAAGCATTTTCTTCCTCTGCTGGTCGCCCTACCACATTGTC
  ATCTTCCTAGATACACTGGAGAGGCTGAAGGCTGTGAATAGCAGCTGCGAGCTGAGTGGCTATCTCTCTG
  TGGCCATCACCTTGTGTGAATTCCTGGGCCTGGCACACTGCTGTCTCAATCCCATGCTCTACACCTTCGC
  TGGCGTAAAGTTCCGCAGTGACCTCTCTCGGCTTCTGACCAAGCTGGGCTGTGCTGGCCCGGCCTCCCTT
  TGCCAACTTTTCCCCAACTGGCGCAAGAGTAGTCTCTCTGAGTCAGAGAATGCTACTTCCCTCACCACCT
  TC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205790 representing NM_007551
  Red=Cloning site Green=Tags(s)
  
  MNYPLTLDMGSITYNMDDLYKELAFYSNSTEIPLQDSNFCSTVEGPLLTSFKAVFMPVAYSLIFLLGMMG
  NILVLVILERHRHTRSSTETFLFHLAVADLLLVFILPFAVAEGSVGWVLGTFLCKTVIALHKINFYCSSL
  LLACIAVDRYLAIVHAVHAYRRRRLLSIHITCTAIWLAGFLFALPELLFAKVGQPHNNDSLPQCTFSQEN
  EAETRAWFTSRFLYHIGGFLLPMLVMGWCYVGVVHRLLQAQRRPQRQKAVRVAILVTSIFFLCWSPYHIV
  IFLDTLERLKAVNSSCELSGYLSVAITLCEFLGLAHCCLNPMLYTFAGVKFRSDLSRLLTKLGCAGPASL
  CQLFPNWRKSSLSESENATSLTTF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007551
• ORF Size: 1122 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007551.2, NP_031577.2
• RefSeq Size: 2636 bp
• RefSeq ORF: 1125 bp
• Locus ID: 12145
• UniProt ID: Q04683
• Cytogenetics: 9 24.84 cM
• Gene Summary: Cytokine receptor that binds to B-lymphocyte chemoattractant (BLC). Involved in B-cell migration into B-cell follicles of spleen and Peyer patches but not into those of mesenteric or peripheral lymph nodes.[UniProtKB/Swiss-Prot Function]