Pdhb (BC019512) Mouse Tagged ORF Clone

CAT#: MG205484

Pdhb (tGFP-tagged) - Mouse pyruvate dehydrogenase (lipoamide) beta (cDNA clone MGC:28576 IMAGE:4210204)

Product Images

Circular map for MG205484

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pdhb
• Synonyms: 2610103L06Rik; AL024199; C81408
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205484 representing BC019512
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGTGGTGGCTGGTCTGGTGCGGGGACCCTTGCGGCAGGCTTCCGGGCTGCTGAAGAGGCGTTTTC
  ACCGCTCGGCGCCCGCAGCGGTGCAGTTGACAGTTCGTGAAGCTATTAATCAAGGTATGGATGAAGAACT
  AGAAAGAGATGAAAAAGTTTTTCTGCTTGGGGAAGAAGTTGCCCAGTATGACGGTGCATACAAGGTTAGC
  AGAGGCCTGTGGAAGAAATACGGTGACAAGAGGATCATCGACACCCCCATCTCAGAGATGGGCTTTGCTG
  GAATTGCTGTTGGTGCAGCTATGGCTGGGTTGCGGCCTATCTGTGAATTTATGACCTTCAATTTCTCTAT
  GCAAGCTATTGACCAGGTTATAAACTCAGCTGCCAAGACTTACTATATGTCCGCTGGCCTTCAGCCTGTG
  CCCATAGTATTCAGGGGGCCCAATGGTGCCTCAGCAGGTGTAGCTGCTCAGCACTCGCAATGCTTTGCTG
  CATGGTATGGGCACTGCCCAGGTTTAAAAGTGGTCAGCCCCTGGAATTCCGAGGATGCAAAAGGACTTAT
  TAAATCGGCCATTCGTGATAATAACCCAGTGGTAATGCTAGAAAATGAACTGATGTATGGAGTTGCATTT
  GAACTTCCTGCAGAAGCTCAGTCAAAAGATTTCCTGATTCCTATTGGAAAAGCCAAAATTGAAAGGCAAG
  GGACCCACATCACTGTAGTTGCCCATTCAAGACCAGTGGGCCACTGCCTAGAAGCTGCAGCTGTATTGTC
  TAAGGAGGGAATTGAATGTGAGGTAATAAATCTGCGCACCATCAGACCAATGGACATCGAAGCCATAGAA
  GCCAGTGTGATGAAGACAAACCATCTCGTGACTGTGGAAGGAGGCTGGCCACAATTTGGAGTAGGAGCTG
  AGATTTGTGCCAGAATTATGGAAGGCCCTGCATTCAACTTCCTTGATGCCCCTGCCGTGCGTGTCACTGG
  TGCTGATGTCCCTATGCCTTACGCAAAGGTCCTAGAAGACAACTCCGTACCTCAGGTCAAAGACATCATA
  TTTGCAGTAAAGAAGACTCTGAACATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205484 representing BC019512
  Red=Cloning site Green=Tags(s)
  
  MAVVAGLVRGPLRQASGLLKRRFHRSAPAAVQLTVREAINQGMDEELERDEKVFLLGEEVAQYDGAYKVS
  RGLWKKYGDKRIIDTPISEMGFAGIAVGAAMAGLRPICEFMTFNFSMQAIDQVINSAAKTYYMSAGLQPV
  PIVFRGPNGASAGVAAQHSQCFAAWYGHCPGLKVVSPWNSEDAKGLIKSAIRDNNPVVMLENELMYGVAF
  ELPAEAQSKDFLIPIGKAKIERQGTHITVVAHSRPVGHCLEAAAVLSKEGIECEVINLRTIRPMDIEAIE
  ASVMKTNHLVTVEGGWPQFGVGAEICARIMEGPAFNFLDAPAVRVTGADVPMPYAKVLEDNSVPQVKDII
  FAVKKTLNI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC019512
• ORF Size: 1079 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC019512, AAH19512
• RefSeq Size: 1467 bp
• RefSeq ORF: 1079 bp
• Locus ID: 68263
• Cytogenetics: 14 A1
• Gene Summary: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.[UniProtKB/Swiss-Prot Function]