Tomm40l (NM_001037170) Mouse Tagged ORF Clone

CAT#: MG204359

Tomm40l (tGFP-tagged) - Mouse translocase of outer mitochondrial membrane 40 homolog-like (yeast) (Tomm40l)

Product Images

Circular map for MG204359

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tomm40l
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204359 representing NM_001037170
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAACACCCTGGGCCTGGCACCGATGGGTACTTTACCCCGCCGGAGCCACCGTCGAGAGGAACCGC
  TGCCCAACCCCGGGAGCTTCGACGAGCTGCACCGGTTGTGCAAAGATGTATTCCCAGCACAGATGGAAGG
  CGTGAAGCTGGTTGTCAACAAGGTTCTGAGCAGCCATTTCCAGGTGGCTCATACTGTGCACATGAGTGCC
  CTGGGCTTGCCAGGGTACCACCTCCATACCGCCTATGCAGGGGACTGGCAGCTTAGCCCCACAGAGGTGT
  TCCCCACTGTGGTGGGGGATATGGACAGCAGTGGCAGTCTCAACGCCCAGGTCTTGCTTCTGTTGGCAGA
  ACGGCTGCGAGCTAAGGCTGTCTTCCAGACACAGCAGGCCAAGTTCCTGACATGGCAGTTTGATGGCGAG
  TATCGGGGAGATGACTACACAGCCACTCTGACCCTGGGAAATCCGGATCTGATTGGGGAATCAGTGATCA
  TGGTTGCTCACTTCCTGCAGAGCATCACCCATCGGCTTGTGCTAGGAGGAGAGCTAGTTTATCACCGGCG
  GCCAGGAGAAGAGGGGGCCATCTTGACTCTGGCTGGGAAGTACTCAGCTGTCCACTGGGTAGCTACGTTG
  AATGTTGGGTCAGGTGGGGCCCATGCAAGTTATTACCACAAGGCCAATGAACAGGTTCAGGTTGGAGTAG
  AGTTTGAGGCAAATACAAGGCTACAAGATACGACCTTCTCCTTTGGTTACCACCTGACTCTGCCCCAGGC
  CGATATGGTGTTTAGAGGCTTGGTGGACAGTAACTGGTGTGTTGGTGCGGTGCTGGAGAAGAAGATGCGT
  CCCCTGCCTGTCACCCTAGCCCTTGGAGCCTTCCTCAATCACTGGCGAAACAGATTCCATTGCGGCTTCA
  GCATCACTGTGGGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204359 representing NM_001037170
  Red=Cloning site Green=Tags(s)
  
  MGNTLGLAPMGTLPRRSHRREEPLPNPGSFDELHRLCKDVFPAQMEGVKLVVNKVLSSHFQVAHTVHMSA
  LGLPGYHLHTAYAGDWQLSPTEVFPTVVGDMDSSGSLNAQVLLLLAERLRAKAVFQTQQAKFLTWQFDGE
  YRGDDYTATLTLGNPDLIGESVIMVAHFLQSITHRLVLGGELVYHRRPGEEGAILTLAGKYSAVHWVATL
  NVGSGGAHASYYHKANEQVQVGVEFEANTRLQDTTFSFGYHLTLPQADMVFRGLVDSNWCVGAVLEKKMR
  PLPVTLALGAFLNHWRNRFHCGFSITVG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001037170
• ORF Size: 924 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001037170.2
• RefSeq Size: 2921 bp
• RefSeq ORF: 927 bp
• Locus ID: 641376
• UniProt ID: Q9CZR3
• Cytogenetics: 1 H3
• Gene Summary: Potential channel-forming protein implicated in import of protein precursors into mitochondria.[UniProtKB/Swiss-Prot Function]