Abhd11 (NM_145215) Mouse Tagged ORF Clone

CAT#: MG204338

Abhd11 (tGFP-tagged) - Mouse abhydrolase domain containing 11 (Abhd11)

Product Images

Circular map for MG204338

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Abhd11
• Synonyms: 1110054D16Rik; A630008N09Rik; Wbscr21
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204338 representing NM_145215
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCCGCTGGGCGCGAGCGTGGAGGGTCCCCCGTGGGGTACTCGGTGCCTCCTCTCCCAGGCGTTTGG
  CGGTTCCTGTCACGTTCTGTAGCAGCCGCAGTAGTGGCCAAGAAAACGCCGATCTGAGGCCGCTGCCGCT
  GTCTTATAATCTTCTAGATGGAGACGCGACACTCCCAGCCATCGTCTTTTTGCATGGGCTCTTCGGCAGC
  AAAACCAACTTCAACTCCCTCGCCAAGGCAATGGTTCAGAGGACTGGCCGGAGGGTGCTGACGGTGGATG
  CCCGGAACCACGGTGACAGCCCCCACAGTCCAGACGCAAGCTATGAGGCCATGAGTCAGGACCTTCAGGG
  CCTCCTTCCACAGCTGGGTCTGGTGCCCTGCGTCCTCGTTGGCCACAGCATGGGAGGAAAGACAGCCATG
  CTCCTGGCACTGCAGAGGCCAGATGTTGTGGAACGATTGGTTGTTGTAGACATAAGCCCGGTAGGAACCA
  CACCTGGATCACACATTGGAGCCTTCATAGCAGCCATGAAGGCTGTTGAGATCCCAGAGAAGGTGCCGCA
  CTCCCAAGCCCGAAAACTGGCGGATAAGCAGCTTAGTTCGGTTGTCAAGGAAGCGGGCATTCGGCAGTTC
  CTGCTCACTAACCTGGTAGAGGTGGGTGGGCGATTCTCCTGGAGACTGAACTTGGATACGTTAGCTCAGC
  ACTTGGACAAGATAATGACCTTTCCTCAGCAACGTGAACCCTACTCGGGGCCAACTCTCTTCCTGCTTGG
  TGGAAATTCTACATATGTACAACCCAGCCACCACTCAGAGATTAGGCGACTCTTCCCTCAAGCCCAGATT
  CAGACCGTGCCTAACGCTGGCCACTGGGTCCACAGCGACAAACCCCAAGACTTCATGGATGCTGTCACCA
  GCTTCTTGGCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204338 representing NM_145215
  Red=Cloning site Green=Tags(s)
  
  MLRWARAWRVPRGVLGASSPRRLAVPVTFCSSRSSGQENADLRPLPLSYNLLDGDATLPAIVFLHGLFGS
  KTNFNSLAKAMVQRTGRRVLTVDARNHGDSPHSPDASYEAMSQDLQGLLPQLGLVPCVLVGHSMGGKTAM
  LLALQRPDVVERLVVVDISPVGTTPGSHIGAFIAAMKAVEIPEKVPHSQARKLADKQLSSVVKEAGIRQF
  LLTNLVEVGGRFSWRLNLDTLAQHLDKIMTFPQQREPYSGPTLFLLGGNSTYVQPSHHSEIRRLFPQAQI
  QTVPNAGHWVHSDKPQDFMDAVTSFLA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145215
• ORF Size: 1490 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145215.2, NP_660250.1
• RefSeq Size: 1505 bp
• RefSeq ORF: 924 bp
• Locus ID: 68758
• UniProt ID: Q8K4F5
• Cytogenetics: 5 G2