Mtap (NM_024433) Mouse Tagged ORF Clone

CAT#: MG203838

Mtap (tGFP-tagged) - Mouse methylthioadenosine phosphorylase (Mtap)

Product Images

Circular map for MG203838

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Mtap
• Synonyms: 1300019I21Rik; MSAP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG203838 representing NM_024433
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCGGGCTCCGCCTGCACGGCGGTGAAGATTGGAATAATTGGTGGAACAGGCTTGGATGATCCCG
  AAATTTTAGAAGGAAGAACTGAGAAATATGTGGATACTCCATTCGGCAAGCCATCCGATGCCTTAATTTT
  GGGGAAGATAAAGAACGTTGATTGTGTACTTCTTGCAAGACACGGCAGACAACACACCATCATGCCTTCA
  AAAGTCAACTACCAGGCAAACATCTGGGCCTTGAAGGAAGAGGGCTGTACACATGTCATCGTGACCACAG
  CTTGCGGGTCCTTGAGAGAGGAGATCCAGCCTGGTGACATGGTCATCATTGACCAATTCATTGACAGGAC
  ATCCCTAAGGCCTCAGACCTTCTATGATGGGAGTCATTGCAGTGCCAGAGGAGTGTGCCACATCCCCATG
  GCTGAACCGTTTTGCCCCAAAACAAGAGAGGTCCTCATAGAAACTGCTAAGAAGCTAGGACTTCGGTGCC
  ATTCAAAGGGGACAATAGTCACAATTGAGGGGCCCCGTTTCAGCTCCCGGGCAGAAAGCCTTATATTCCG
  TACTTGGGGCGCAGATGTTGTCAACATGACCACTGTTCCAGAGGTGGTACTTGCTAAGGAGGCTGGAATT
  TGCTATGCAAGCATTGCCATGGCAACCGACTATGATTGTTGGAAGGAGCATGAAGAAGCAGTGTCAGTGG
  ATGGGGTTTTAAAGACCATGAAAGAAAATGCCAATAAAGCCAAAAGTTTACTCCTCACTACTATACCTCA
  GATAGGGTCGATGGAATGGTCAGAAACGCTCCGTAACCTAAAGAACATGGCCCAGTTTTCCGTTTTACCA
  CCAAGACAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG203838 representing NM_024433
  Red=Cloning site Green=Tags(s)
  
  MASGSACTAVKIGIIGGTGLDDPEILEGRTEKYVDTPFGKPSDALILGKIKNVDCVLLARHGRQHTIMPS
  KVNYQANIWALKEEGCTHVIVTTACGSLREEIQPGDMVIIDQFIDRTSLRPQTFYDGSHCSARGVCHIPM
  AEPFCPKTREVLIETAKKLGLRCHSKGTIVTIEGPRFSSRAESLIFRTWGADVVNMTTVPEVVLAKEAGI
  CYASIAMATDYDCWKEHEEAVSVDGVLKTMKENANKAKSLLLTTIPQIGSMEWSETLRNLKNMAQFSVLP
  PRH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024433
• ORF Size: 849 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024433.2
• RefSeq Size: 2565 bp
• RefSeq ORF: 852 bp
• Locus ID: 66902
• UniProt ID: Q9CQ65
• Cytogenetics: 4 C4
• Gene Summary: Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.[UniProtKB/Swiss-Prot Function]