Uqcrfs1 (NM_025710) Mouse Tagged ORF Clone

CAT#: MG203679

Uqcrfs1 (tGFP-tagged) - Mouse ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 (Uqcrfs1)

Product Images

Circular map for MG203679

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Uqcrfs1
• Synonyms: 4430402G14Rik; AI875505
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG203679 representing NM_025710
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGTCGGTCGCCGCCCGCTCGGGCCCGTTCGCGCCCGTCCTATCGGCCACTTCCCGCGGGGTGGCGG
  GCGCGCTGCGTCCTCTGCTGCAAGGCGCGGTGCCCGCCGCCTCGGAGCCACCTGTTCTGGATGTGAAGCG
  ACCCTTCCTGTGCCGCGAGTCCCTGAGTGGCCAGGCCGCAGCCCGGCCTTTGGTGGCCACGGTGGGCCTG
  AACGTTCCTGCTTCTGTCCGTTTTTCCCATACAGATGTCAAGGTGCCCGACTTCTCTGACTATCGTCGTG
  CTGAAGTTCTTGATAGCACAAAATCTTCTAAAGAAAGCAGTGAGGCTAGAAAAGGCTTCTCTTACCTGGT
  AACTGCAACTACTACTGTGGGTGTTGCTTATGCGGCCAAAAATGTGGTCTCCCAGTTTGTTTCCAGCATG
  AGTGCTTCTGCTGACGTACTGGCCATGTCGAAGATCGAGATCAAGTTGTCTGATATCCCTGAAGGGAAGA
  ACATGGCTTTTAAATGGAGAGGCAAACCTCTGTTTGTGCGCCATAGAACCAAGAAGGAGATTGACCAGGA
  AGCTGCAGTCGAAGTGTCCCAGTTAAGGGACCCACAGCATGATTTAGATCGAGTAAAGAAACCTGAATGG
  GTTATTCTGATAGGTGTCTGTACTCATCTTGGTTGTGTACCCATTGCAAACGCAGGAGATTTTGGTGGCT
  ATTATTGCCCCTGCCATGGGTCACACTATGACGCCTCTGGCAGGATCAGGAAGGGCCCTGCACCTCTCAA
  CCTGGAAGTGCCTGCCTACGAGTTCACCAGTGATGATGTAGTTGTTGTGGGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG203679 representing NM_025710
  Red=Cloning site Green=Tags(s)
  
  MLSVAARSGPFAPVLSATSRGVAGALRPLLQGAVPAASEPPVLDVKRPFLCRESLSGQAAARPLVATVGL
  NVPASVRFSHTDVKVPDFSDYRRAEVLDSTKSSKESSEARKGFSYLVTATTTVGVAYAAKNVVSQFVSSM
  SASADVLAMSKIEIKLSDIPEGKNMAFKWRGKPLFVRHRTKKEIDQEAAVEVSQLRDPQHDLDRVKKPEW
  VILIGVCTHLGCVPIANAGDFGGYYCPCHGSHYDASGRIRKGPAPLNLEVPAYEFTSDDVVVVG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025710
• ORF Size: 822 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025710.2
• RefSeq Size: 1316 bp
• RefSeq ORF: 825 bp
• Locus ID: 66694
• UniProt ID: Q9CR68
• Cytogenetics: 13 A3.2
• Gene Summary: Cytochrome b-c1 complex subunit Rieske, mitochondrial: Component of the mitochondrial ubiquinol-cytochrome c reductase complex dimer (complex III dimer), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis (PubMed:28673544). Incorporation of UQCRFS1 is the penultimate step in complex III assembly (By similarity).[UniProtKB/Swiss-Prot Function]