Furin (NM_011046) Mouse Untagged Clone

CAT#: MC228946

Furin (untagged) - Mouse furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Furin
• Synonyms: 9130404I01Rik; Fu; Fur; PA; PACE; Pcs; Pcsk3; SP; SPC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228946 representing NM_011046
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTGAGATCCTGGTTGCTATGGGTGGTCGCAGCAGCAGGAGCCGTGGTCCTGCTGGCAGCTGATG
  CTCAAGGCCAGAAGATCTTCACCAACACCTGGGCCGTGCACATTCCTGGAGGCCCAGCTGTGGCTGATAG
  GGTGGCGCAGAAGCATGGCTTCCACAACCTGGGCCAGATCTTCGGTGACTATTACCACTTCTGGCACAGA
  GCAGTGACAAAGCGGTCCCTGTCGCCTCACCGCCCGCGGCACAGCCGGCTACAGAGGGAGCCTCAAGTAA
  AGTGGCTGGAGCAGCAGGTAGCCAAGCGAAGAGCCAAGAGGGACGTGTATCAGGAGCCCACGGACCCCAA
  GTTCCCCCAGCAGTGGTACCTGTCTGGTGTCACTCAGCGAGACCTGAATGTGAAGGAGGCCTGGGCCCAG
  GGCTTCACAGGCCATGGCATTGTGGTCTCCATCCTGGATGACGGCATTGAGAAGAATCATCCCGACCTAG
  CAGGCAATTATGACCCTGGAGCCAGTTTTGACGTGAATGACCAGGACCCCGACCCACAGCCTCGGTACAC
  ACAGATGAATGACAACAGGCATGGCACTCGCTGTGCCGGGGAAGTGGCAGCAGTGGCCAACAATGGTGTC
  TGTGGCGTAGGTGTAGCTTACAATGCCCGAATTGGAGGGGTGCGGATGTTGGATGGCGAGGTGACTGATG
  CAGTAGAGGCACGTTCGCTGGGCCTGAATCCCAACCACATCCACATCTACAGCGCCAGCTGGGGCCCTGA
  GGACGACGGCAAGACCGTGGATGGACCAGCCCGGCTCGCTGAGGAGGCCTTCTTTCGGGGAGTTAGCCAG
  GGCCGAGGAGGGCTGGGCTCCATCTTTGTCTGGGCCTCAGGGAATGGGGGCCGGGAACATGACAGCTGCA
  ACTGTGACGGCTACACCAACAGCATCTATACACTGTCCATCAGCAGCGCCACACAGTTCGGCAATGTGCC
  CTGGTACAGTGAGGCCTGCTCCTCCACACTGGCCACCACCTACAGCAGCGGCAACCAGAATGAGAAGCAG
  ATCGTGACAACTGACCTGAGGCAGAAGTGCACAGAATCTCACACAGGCACCTCGGCTTCTGCCCCCTTGG
  CAGCTGGTATCATTGCTCTCACCCTGGAGGCCAACAAGAACCTCACCTGGCGGGACATGCAGCACCTGGT
  AGTGCAGACCTCAAAGCCAGCCCACCTCAACGCTGATGATTGGGCTACCAACGGCGTGGGCCGGAAAGTG
  AGCCATTCGTATGGCTACGGGCTGTTGGATGCAGGTGCCATGGTGGCTCTGGCCCAGAACTGGACAACAG
  TGGCCCCCCAGCGGAAGTGCATTGTTGAAATCCTGGTGGAACCCAAGGACATCGGCAAACGGCTAGAGGT
  GCGCAAGGCGGTGACAGCATGCCTGGGTGAGCCCAACCACATCACCAGGCTGGAACACGTTCAGGCCCGG
  CTCACCCTGTCTTACAATCGCCGCGGTGACCTGGCTATCCACCTCATCAGCCCCATGGGCACCCGCTCCA
  CCCTGTTAGCTGCCAGACCACATGACTACTCTGCTGATGGGTTTAATGATTGGGCTTTCATGACAACCCA
  TTCCTGGGATGAGGATCCGGCTGGCGAATGGGTCCTAGAGATTGAAAATACCAGTGAAGCCAACAACTAT
  GGGACGCTGACCAAGTTCACTCTCGTTCTGTATGGCACAGCCCCCGAGGGGCTCTCTACACCTCCAGAAA
  GCAGCGGCTGCAAGACCCTCACATCCAGCCAGGCCTGCGTGGTGTGTGAGGAAGGTTACTCTCTGCACCA
  GAAAAGCTGTGTCCAGCACTGCCCACCAGGCTTCATCCCCCAAGTCCTTGATACACACTACAGCACTGAG
  AATGATGTGGAGATCATCCGTGCCAGCGTCTGTACCCCCTGCCACGCCTCATGTGCCACTTGCCAGGGCC
  CGGCCCCCACAGACTGCCTCAGCTGCCCCAGCCATGCCTCCCTGGACCCAGTGGAGCAGACCTGCTCCCG
  GCAAAGCCAGAGCAGCCGGGAGTCTCGGCCTCAGCAGCAGCCTCCTGCACTGCGCCCTGAGGTGGAGATG
  GAGCCCCGGCTGCAGGCTGGGTTGGCCTCTCACCTGCCCGAGGTCCTGGCCGGCCTCAGCTGCCTTATCA
  TCGTGCTCATCTTTGGCATCGTCTTCCTGTTCCTGCATCGGTGTTCGGGCTTCAGCTTCCGGGGAGTGAA
  AGTGTACACCATGGACCGCGGCCTCATCTCCTACAAGGGGCTGCCCCCTGAAGCCTGGCAGGAGGAGTGC
  CCATCGGACTCAGAGGAGGACGAGGGCCGGGGCGAGAGGACCGCCTTCATCAAAGACCAGAGCGCCCTTT
  GA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011046
• Insert Size: 2382 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011046.3, NP_035176.1
• RefSeq Size: 4336 bp
• RefSeq ORF: 2382 bp
• Locus ID: 18550
• UniProt ID: P23188
• Cytogenetics: 7 45.65 cM
• Gene Summary: This gene encodes a calcium-dependent serine endoprotease that proteolytically activates different proprotein substrates traversing the secretory pathway. The encoded protein undergoes proteolytic autoactivation during which an N-terminal propeptide is cleaved to generate the mature protein. Mice lacking the encoded protein die at an embryonic stage and display hemodynamic insufficiency, cardiac ventral closure defect, axial rotation defect and abnormal yolk sac vasculature. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.