Arntl (NM_001243048) Mouse Untagged Clone

CAT#: MC228467

Arntl (untagged) - Mouse aryl hydrocarbon receptor nuclear translocator-like (Arntl), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Arntl
• Synonyms: Arnt3; bHLHe5; Bmal1; BMAL1b; bmal1b'; MOP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228467 representing NM_001243048
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGACCAGAGAATGGACATTTCCTCAACCATCAGCGACTTCATGTCTCCGGGCCCCACCGACCTAC
  TCTCCGGTTCCCTGGGCACCAGTGGTGTGGACTGCAATCGCAAGAGGAAAGGCAGTGCCACTGACTACCA
  GTTAGAATATGCAGAACACCAAGGAAGGATCAAGAATGCAAGGGAGGCCCACAGTCAGATTGAAAAGAGG
  CGTCGGGACAAAATGAACAGTTTCATTGATGAATTGGCTTCTTTGGTACCAACATGCAATGCAATGTCCA
  GGAAGTTAGATAAACTCACCGTGCTAAGGATGGCTGTTCAGCACATGAAAACTTTGAGAGGTGCCACCAA
  CCCATACACAGAAGCAAACTACAAGCCAACATTTCTATCAGATGACGAACTGAAACACCTAATTCTCAGG
  GCAGCAGATGGATTTTTGTTTGTCGTAGGATGTGACCGAGGGAAGATCCTCTTTGTCTCCGAGTCTGTCT
  TCAAGATCCTCAATTATAGCCAGAATGACCTTATTGGCCAGAGCTTGTTTGACTACCTGCATCCAAAAGA
  TATTGCCAAAGTTAAGGAACAGCTATCTTCCTCGGACACTGCGCCCCGGGAGCGACTCATTGATGCCAAG
  ACTGGACTTCCGGTTAAAACGGATATAACCCCTGGGCCCTCCCGGCTATGCTCTGGAGCCCGCCGCTCTT
  TCTTCTGTAGAATGAAGTGCAACAGGCCTTCAGTAAAGGTGGAAGATAAGGACTTCGCCTCTACCTGTTC
  AAAGAAAAAAGCAGATCGAAAAAGCTTCTGCACAATCCACAGCACAGGCTATTTGAAAAGCTGGCCACCC
  ACGAAGATGGGGCTGGACGAAGACAATGAGCCAGACAACGAGGGCTGCAACCTCAGCTGCCTCGTTGCAA
  TCGGGCGCCTGCACTCGCACATGGTTCCACAACCAGCGAACGGGGAAATACGGGTGAAATCTATGGAGTA
  CGTTTCTCGACACGCAATAGATGGGAAATTTGTTTTTGTAGATCAGAGGGCGACAGCTATTTTGGCGTAT
  CTACCACAGGAACTTCTAGGTACATCATGTTATGAGTATTTTCATCAAGACGACATAGGACACCTCGCAG
  AATGTCACAGGCAAGTTTTACAGACAAGAGAAAAGATCACAACTAATTGCTATAAGTTTAAGATCAAAGA
  TGGTTCTTTTATCACGCTACGAAGTCGATGGTTCAGTTTCATGAACCCGTGGACCAAGGAAGTTGAATAC
  ATTGTCTCAACCAACACTGTTGTTTTAGCCAATGTCCTGGAAGGCGGGGACCCAACCTTCCCGCAGCTAA
  CAGCACCCCCCCACAGCATGGACAGCATGCTGCCCTCTGGAGAAGGTGGCCCAAAGAGGACTCATCCCAC
  TGTCCCAGGCATTCCAGGGGGAACCAGAGCCGGAGCAGGAAAAATAGGTCGAATGATCGCGGAGGAAATC
  ATGGAAATCCACAGGATAAGAGGGTCATCGCCTTCCAGCTGTGGCTCCAGCCCGCTGAACATCACAAGTA
  CGCCTCCCCCTGATGCCTCTTCTCCAGGAGGCAAGAAGATTCTAAATGGAGGGACTCCAGACATTCCTTC
  CACTGGACTATTACCAGGGCAGGCTCAGGAGACCCCAGGGTATCCCTATTCTGATAGTTCTTCTATTCTT
  GGTGAGAACCCCCACATAGGCATCGATATGATAGATAACGACCAAGGATCAAGTAGTCCCAGTAACGATG
  AGGCAGCAATGGCTGTCATCATGAGCCTCTTGGAAGCAGATGCGGGGCTGGGTGGCCCCGTTGACTTTAG
  TGACTTGCCATGGCCGCTGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001243048
• Insert Size: 1842 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001243048.1, NP_001229977.1
• RefSeq Size: 2628 bp
• RefSeq ORF: 1842 bp
• Locus ID: 11865
• UniProt ID: Q9WTL8
• Cytogenetics: 7 59.17 cM
• Gene Summary: The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with Clock. This heterodimer binds E-box enhancer elements upstream of Period (Per1, Per2, Per3) and Cryptochrome (Cry1, Cry2) genes and activates transcription of these genes. Per and Cry proteins heterodimerize and repress their own transcription by interacting in a feedback loop with Clock/Arntl complexes. Defects in this gene have been linked to infertility, problems with gluconeogenesis and lipogenesis, and altered sleep patterns. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2014]
  Transcript Variant: This variant (2) lacks several in-frame exons in the coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.