Tars2 (NM_027931) Mouse Untagged Clone

CAT#: MC221104

Tars2 (untagged) - Mouse threonyl-tRNA synthetase 2, mitochondrial (putative) (Tars2), nuclear gene encoding mitochondrial protein, transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tars2
• Synonyms: 2610024N01Rik; AI429208; Tarsl1; thrRS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221104 representing NM_027931
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTCTCTGTCTGAGGTGGCGCCGGCTTGGGTTCCCACTCCCAGAGTTCCGCCGCTGCGAGCTCCACA
  CCGTGCGTGAGGCCTCTGCACCAACTCCTCCACATTGGTTGGCAGAACGATTTGGCCTTTTTGAGGAGCT
  ATGGACCGCTCACGTGAAAAAGTTAGCAAGTATGACACAGAAGAAAGCCCGGGCTATTAAGATATCACTT
  CCTGAAGGCCAGAAGGTAGATGCTGTTGCATGGAACACAACCCCTTACCAACTGGCCCATCAGATCAGTG
  TAACACTGGCTGATACTGCAGTGGCTGCTGAAGTAAATGGAGAACTTTACGATCTGGACCGACCCTTGGA
  GACAGATTGTCACCTCAGATTTCTGACATTTGATTCCCCAGAGGGCAAAGCGGTGTTCTGGCACTCTAGT
  GCCCATGTTCTGGGGGCTGCGGCTGAGCAACAACTGGGTGCTGTTCTCTGCCGAGGTCCAAGCACAGAAT
  CGGGCTTTTACCATGACTTCTTCCTGGGAAAAGAACGGACAGTCCGCAGCGCAGAGTTGCCCATTTTAGA
  GCGGATTTGCCAGGAGCTCATAGCTGCTGCACAGCCTTTCCGGAGGCTGGAGGCTTCACGGGATCAGCTT
  CGCCAGCTCTTCAAGGACAACCACTTTAAGCTTCATCTGATCGAGGAGAAAGTGACAGGCCCAACGGCAA
  CAGTGTATGGGTGTGGCATGTCAGTTGACCTGTGCCGAGGCCCCCATCTTCGGCACACTGGACAGATTGG
  AGCACTGAAGCTGCTCACGAACTCCTCAGCCTTGTGGAGGTCCTTGGGAGCACCTGAGACACTGCAGAGG
  GTATCAGGAATTTCTTTCCCCAAAGTAGAGTTACTGAGGAACTGGGAAGCTCGAAGAGAAGCAGCAGAGT
  TAAGAGACCACAGACGCATTGGGAAGGAACAGGAGCTCTTCTTCTTCCATGAACTGAGCCCTGGGAGCTG
  TTTTTTCTTGCCACGAGGGACAAGAGTCTATAATGCCCTGGTGGCTTTCATCAGGGCTGAGTATGCCCGC
  CGTGGTTTCTCAGAGGTGAAAACTCCCACGCTGTTTTCTACAAAACTCTGGGAACAGTCAGGGCACTGGG
  AACACTATAGGGCAGACATGTTTTCCCTGAAGCCCCCTGGCACTGATGGTGTTGACAACTCCCAGAGTGG
  CCATCCTGCCAGGTGTCCCAAAGACACACTTGCTCTAAAGCCCATGAACTGCCCTGCACACTGCCTGATG
  TTTGCCCACCGGCCCAGATCCTGGAGGGAACTGCCTGTGCGACTGGCTGATTTCGGAGCCCTGCATCGGG
  CTGAGGCCTCTGGCAGTCTGGGAGGATTAACGCGGCTGTGGCGCTTCCAGCAGGATGATGCGCACATCTT
  TTGTGCACCCCATCAGCTGGAAGCAGAGATCCAGGGCTGCCTTGATTTTCTCCGGTGTGTTTACTCGGTT
  CTTGGTTTTTCCTTCCACCTGGCTTTATCTACCCGGCCACCTGGTTTCCTAGGGGAGCCTCGCCTATGGG
  ACCAGGCTGAGCAGGTTCTACAGCAAGCCTTGGAGAAGTTTGGAGAACCTTGGGACCTCAACCCTGGAGA
  TGGGGCTTTCTATGGGCCTAAGATTGATGTGCACCTCCACGACGCCCTTGGTCGGCCCCATCAGTGTGGA
  ACAATCCAGCTTGACTTCCAGCTGCCACTGAGATTTGACCTACAATACAAGGGGCCGGCAGGTACCCCAG
  AGTGTCCAGTCCTTATTCATCGAGCAGTGCTTGGTTCTGTGGAAAGGCTGTTGGGAGTGCTGGCAGAAAG
  CTGTGGGGGGAAATGGCCCCTGTGGCTGTCCCCGCTCCAGGTGGTGGTCATCCCTGTGAGGACAGAGCAA
  GAGGAATATGCCAGGCAGGTGCAACAGTGCCTGCAGGCTGCAGGACTTGTGAGTGATCTCGATGCAGACT
  CTGGACTGACCCTCAGCCGGAGAGTCCGCCGGGCCCAGCTTGCCCACTACAACTTTCAGTTTGTGGTTGG
  CCAGAGAGAGCAGAGTCAAAGGACAGTGAACGTACGCACGCGAGATAACCGGCAGCTCGGGGAGCGAGAC
  CTGGCCGAGTCCGTGCAGAGACTGCTGGAGTTACAGAACGCCAGGGTCCCCAATGCAGAAGAAGTGTTCT
  GA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_027931
• Insert Size: 2172 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027931.3, NP_082207.2
• RefSeq Size: 2524 bp
• RefSeq ORF: 2172 bp
• Locus ID: 71807
• UniProt ID: Q3UQ84
• Cytogenetics: 3 F2.1
• Gene Summary: Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction: threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr). Also edits incorrectly charged tRNA(Thr) via its editing domain.[UniProtKB/Swiss-Prot Function]